Extracellular matrix of the bovine ovarian membrana granulosa

Rodgers, Raymond J.; Rodgers, Helen F.

doi:10.1016/s0303-7207(02)00057-6

Cited by 26 publications

(29 citation statements)

References 54 publications

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“…The kit ligand (KL), secreted by granulosa cells, has been shown to stimulate cell growth and androgen synthesis in the bovine theca interna in vitro (Parrot & Skinner 1997). Moreover, it has been recently implied that the interaction between granulosa and thecal cells depends not only on the paracrine communication between them, but also on their contact with the basal lamina (Rodgers & Irving-Rodgers 2002, Allegrucci et al 2003.…”

Section: Discussionmentioning

confidence: 99%

Gene expression for LH receptor, 17α-hydroxylase and StAR in the theca interna of preantral and early antral follicles in the bovine ovary

Braw–Tal

Roth²

2005

Reproduction

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The onset of gene expression for three proteins that play pivotal roles in theca interna function, namely the LH receptor (LH-R), cytochrome P450 17a-hydroxylase (17aOH) and the steroidogenic acute regulatory protein (StAR), was determined. Ovaries were obtained on day 9 of the oestrus cycle from mature synchronized dairy cows (n 5 5) and gene expression in preantral and antral follicles up to 4 mm in diameter was evaluated by in situ hybridization. LH-R and 17aOH mRNAs were observed first, in the theca interna of large preantral follicles (type 4), concurrent with its morphological differentiation. StAR mRNA appeared later during follicular growth, in follicles >1 mm in diameter (type 6). LH-R and 17aOH mRNAs were found exclusively in the thecal cells, whereas StAR mRNA appeared in thecal cells, granulosa cells of late atretic follicles and oocytes. In early atresia, thecal cells expressed all three mRNAs, and their expression decreased gradually as atresia progressed. Atresia in granulosa cells was characterized by massive apoptosis of periantral, but not peribasal cells, that differentiated into luteallike cells expressing StAR. In summary, our study suggests that in spite of the presence of 17aOH, a key enzyme in steroidogenesis, the ability to produce steroids by bovine follicles smaller than 1 mm in diameter must be very limited due to the absence of StAR protein.During the early stages of atresia, thecal cells remain morphologically and functionally healthy, and continue to express all three studied mRNAs.

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Section: Discussionmentioning

confidence: 99%

Gene expression for LH receptor, 17α-hydroxylase and StAR in the theca interna of preantral and early antral follicles in the bovine ovary

Braw–Tal

Roth²

2005

Reproduction

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“…It has been demonstrated that the extracellular matrix (ECM) and its components affect the proliferation and the secretory function of many cultured cell types (14,15) and, in particular, they support the in vitro long-term culture of pancreatic islets (16)(17)(18)(19). Hammar et al (20) have shown that ECM protects pancreatic ß-cells from apoptosis leading to improved cell survival.…”

Section: Introductionmentioning

confidence: 99%

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

Carlo

Baiguera²,

Conconi³

et al. 2009

Int J Mol Med

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Abstract. Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matrix or on the liver matrix showed a progressive reduction. In order to obtain implantable devices, acellular matrix/islet cultures were entrapped into poly(vinyl alcohol) (PVA)/ poly(ethylene glycol) (PEG) tubes obtained by the freezing/thawing procedure. Under this condition, an in vitro constant insulin release was detected. The devices were then implanted into diabetic rats where reduced insulin requirement was noted suggesting insulin secretory activity of islets contained in the device. Indeed, immunofluorescence confirmed the presence of insulin-and glucagon-producing cells into the explanted devices. These data show that PVA/PEG semi-permeable membrane can obtain devices that restore, at least in part, insulin secretion.

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“…It is believed to influence granulosa cell proliferation and differentiation, to exclude capillaries and white blood cells until ovulation, and to filter the molecules entering and exiting the follicle. Evidence suggests that granulosa cells produce many of the components of the follicular basal lamina (reviewed by Rodgers and Irving Rodgers, 2002).…”

Section: The Basal Laminamentioning

confidence: 99%

“…Changes in the structure of basal laminae from preantral follicles and antral follicles (Rodgers and Irving Rodgers, 2002), and the increased concentration of serum-derived proteins in the follicular fluid (Russell and Salustri, 2006)-necessary for COC expansion (see below)-suggest that this is the case.…”

Section: The Basal Laminamentioning

confidence: 99%

The antral follicle: a microenvironment for oocyte differentiation

Hennet

Combelles²

2012

Int. J. Dev. Biol.

152

119

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Mammalian reproduction hinges upon the timely ovulation of a fully differentiated oocyte. This event is the culmination of a complex and dynamic developmental relationship between the oocyte and the antral follicle housing it; the antral follicle constitutes a specialized microenvironment or niche, uniquely suited to the needs of the oocyte as it approaches ovulation. During this time, the oocyte must complete its final growth, capacitation, and nuclear and cytoplasmic maturation. Its microenvironment-the antral follicle-is in turn responsible for the integrity of these processes and the production of a high quality oocyte. Components of the antral follicle, including three distinct somatic cell types (theca, granulosa and cumulus), the basal lamina, and follicular fluid, each have active and regulatory roles in oocyte differentiation. Several milestones in antral folliculogenesis also have an influence on oocyte development. This review will discuss the antral follicle microenvironment with specific attention to its importance in oocyte differentiation. As assisted reproductive technologies (ART) often require stages of oocyte differentiation to occur in vitro rather than in vivo, current knowledge of the antral follicle microenvironment will also be discussed with respect to its clinical applications.

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Extracellular matrix of the bovine ovarian membrana granulosa

Cited by 26 publications

References 54 publications

Gene expression for LH receptor, 17α-hydroxylase and StAR in the theca interna of preantral and early antral follicles in the bovine ovary

Gene expression for LH receptor, 17α-hydroxylase and StAR in the theca interna of preantral and early antral follicles in the bovine ovary

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

The antral follicle: a microenvironment for oocyte differentiation

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