Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments

Naqvi, Syeda M.; Buckley, Conor T.

doi:10.1111/joa.12305

Cited by 42 publications

(56 citation statements)

References 49 publications

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“…Despite the IVD-like glucose condition was found a positive factor for ADSCsbased IVD regeneration, the cell viability and proliferation and the matrix biosynthesis capability were inhibited in combined IVD condition. 4,19,20 From this respect, the IVD condition is still an obstacle for the application of cell-based therapy to treat degenerative diseases.…”

Section: Discussionmentioning

confidence: 99%

The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment

Wang

Tao

Zhou

et al. 2016

Exp Biol Med (Maywood)

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Recent studies indicated that cell-based therapy could be a promising approach to treat intervertebral disc degeneration. Though the harsh microenvironment in disc is still challenging to implanted cells, it could be overcome by pre-conditioning graft cells before transplantation, suggested by previous literatures. Therefore, we designed this study to identify the potential effect of chondrogenic pre-differentiation on adipose-derived mesenchymal stem cells in intervertebral disc-like microenvironment, characterized by limited nutrition, acidic, and high osmosis in vitro. Adipose-derived mesenchymal stem cells of rat were divided into five groups, embedded in type II collagen scaffold, and cultured in chondrogenic differentiation medium for 0, 3, 7, 10, and 14 days. Then, the adipose-derived mesenchymal stem cells were implanted and cultured in intervertebral disc-like condition. The proliferation and differentiation of adipose-derived mesenchymal stem cells were evaluated by cell counting kit-8 test, realtime quantitative polymerase chain reaction, and Western blotting and immunofluorescence analysis. Analyzed by the first week in intervertebral disc-like condition, the results showed relatively greater proliferative capability and extracellular matrix synthesis ability of the adipose-derived mesenchymal stem cells pre-differentiated for 7 and 10 days than the control. We concluded that pre-differentiation of rat adipose-derived mesenchymal stem cells in chondrogenic culture medium for 7 to 10 days could promote the regeneration effect of adipose-derived mesenchymal stem cells in intervertebral disc-like condition, and the pre-differentiated cells could be a promising cell source for disc regeneration medicine.

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Section: Discussionmentioning

confidence: 99%

The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment

Wang

Tao

Zhou

et al. 2016

Exp Biol Med (Maywood)

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“…[114][115][116] For in vitro cell culture models, mimicking this in vivo disc microenvironment can be accomplished by culturing cells in low oxygen, and reduced glucose and serum, to simulate nutrient deprivation. [115][116][117] The degenerate environment can be further simulated by including catabolic mediators such as inflammatory cytokines and by increasing acidity. [118][119][120] The physical environment can be mimicked through culturing in soft 3D scaffolds such as hydrogels.…”

Section: Minimizing Potential Risks To Patientsmentioning

confidence: 99%

Advancing cell therapies for intervertebral disc regeneration from the lab to the clinic: Recommendations of the ORS spine section

et al. 2018

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Intervertebral disc degeneration is strongly associated with chronic low back pain, a leading cause of disability worldwide. Current back pain treatment approaches (both surgical and conservative) are limited to addressing symptoms, not necessarily the root cause. Not surprisingly therefore, long‐term efficacy of most approaches is poor. Cell‐based disc regeneration strategies have shown promise in preclinical studies, and represent a relatively low‐risk, low‐cost, and durable therapeutic approach suitable for a potentially large patient population, thus making them attractive from both clinical and commercial standpoints. Despite such promise, no such therapies have been broadly adopted clinically. In this perspective we highlight primary obstacles and provide recommendations to help accelerate successful clinical translation of cell‐based disc regeneration therapies. The key areas addressed include: (a) Optimizing cell sources and delivery techniques; (b) Minimizing potential risks to patients; (c) Selecting physiologically and clinically relevant efficacy metrics; (d) Maximizing commercial potential; and (e) Recognizing the importance of multidisciplinary collaborations and engaging with clinicians from inception through to clinical trials.

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“…23 Importantly, energy metabolism rates are nonlinear coupled reactions and dependent on the local nutrient and acidic microenvironment. 24 Equally relevant from a regenerative medicine or tissue engineering perspective, oxygen concentrations appear to play a key role in regulating the phenotype and biosynthetic activity of cells intended for therapeutic applications, 25 while low glucose concentrations and low pH levels have been found to impair the survival and biological behavior of stem cells. [26][27][28] In addition, the IVD is subjected to biophysical forces in vivo, such as deformational strain and hydrostatic pressure.…”

Section: The Role Of the Disc Microenvironmentmentioning

confidence: 99%

Critical aspects and challenges for intervertebral disc repair and regeneration—Harnessing advances in tissue engineering

et al. 2018

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Low back pain represents the highest burden of musculoskeletal diseases worldwide and intervertebral disc degeneration is frequently associated with this painful condition. Even though it remains challenging to clearly recognize generators of discogenic pain, tissue regeneration has been accepted as an effective treatment option with significant potential. Tissue engineering and regenerative medicine offer a plethora of exploratory pathways for functional repair or prevention of tissue breakdown. However, the intervertebral disc has extraordinary biological and mechanical demands that must be met to assure sustained success. This concise perspective review highlights the role of the disc microenvironment, mechanical and clinical design considerations, function vs mimicry in biomaterial‐based and cell engineering strategies, and potential constraints for clinical translation of regenerative therapies for the intervertebral disc.

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Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments

Abstract: Matrix Production by Nucleus Pulposus and Bone Marrow Stem Cells

Cited by 42 publications

References 49 publications

The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment

The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment

Advancing cell therapies for intervertebral disc regeneration from the lab to the clinic: Recommendations of the ORS spine section

Critical aspects and challenges for intervertebral disc repair and regeneration—Harnessing advances in tissue engineering

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