1997
DOI: 10.1006/clin.1997.4418
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Extracellular Matrix Remodeling in Coxsackievirus B3 Myocarditis

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Cited by 9 publications
(2 citation statements)
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“…The coxsackievirus B3 (CVB3) used in this study was derived from the infectious cDNA copy of the cardiotropic H3 strain (Woodruff variant) of CVB3 (24). Virus titers were determined on HeLa cell monolayers using a standard plaque-forming assay, and virus isolation from heart and liver was performed as described previously (25). Recombinant adenovirus vectors containing the genes for LacZ, Myc-tagged SOCS1, Myc-tagged SOCS3, and Cre recombinase were prepared on 293 cells as described previously (26).…”
Section: Methodsmentioning
confidence: 99%
“…The coxsackievirus B3 (CVB3) used in this study was derived from the infectious cDNA copy of the cardiotropic H3 strain (Woodruff variant) of CVB3 (24). Virus titers were determined on HeLa cell monolayers using a standard plaque-forming assay, and virus isolation from heart and liver was performed as described previously (25). Recombinant adenovirus vectors containing the genes for LacZ, Myc-tagged SOCS1, Myc-tagged SOCS3, and Cre recombinase were prepared on 293 cells as described previously (26).…”
Section: Methodsmentioning
confidence: 99%
“…On the day of assay, the frozen tissue was homogenized in 200 l of PBS and centrifuged at 12,000 rpm for 5 min. Viral titer (expressed in PFU) was determined in the supernatants by a plaque assay method on HeLa cell monolayers as previously described (12).…”
Section: Plaque-forming Assay Determination Of Cardiac Viral Loadmentioning
confidence: 99%