Extracellular Matrix Remodeling of the Umbilical Cord in Pre-eclampsia as a Risk Factor for Fetal Hypertension

Romanowicz, Lech; Galewska, Zofia

doi:10.1155/2011/542695

Cited by 18 publications

(18 citation statements)

References 60 publications

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“…ECM was a dynamic fibrous protein network with mutual interaction between the big molecules, which contained collagen, mucin, fibronectin, proteoglycan, etc. MMPs family was one of the most vital proteases, which could degrade ECM [5]. The degradation of ECM was the key stage of tumor metastasis, mainly under the regulation of MMPs.…”

Section: Discussionmentioning

confidence: 99%

Influence of lymphatic endothelial cells on proliferation and invasiveness of esophageal carcinoma cells in vitro and lymphangiogenesis in vivo

Nana

Sun

et al. 2015

Med Oncol

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The aim of the study was to investigate the interaction between esophageal carcinoma cells with different differentiation degree and esophageal carcinoma-related lymphatic endothelial cells. Different lymphatic endothelial cell conditioned mediums were used to cultivate well-differentiated esophageal carcinoma EC9706 cells and poorly differentiated esophageal carcinoma KYSE150 cells, and immunocytochemistry and Western blot analyses were applied to detect the expression of MMP-9 protein and TIMP-2 protein in each group; in situ hybridization and RT-PCR methods were used to detect the expression of MMP-9 and TIMP-2 mRNA in each group; CCK-8 method was used to detect cell proliferation in each group; and transwell method was utilized to detect cell invasiveness in each group. Through constructing the transplanted tumor model of esophageal carcinoma of nude mice, the D2-40 and LYVE-1 immunohistochemical staining was performed on transplanted tumors and surrounding tissues, lymphatic microvessels were marked, and lymphatic microvessel density (LMVD) was measured. The expression of MMP-9 protein and mRNA in experimental group was significantly higher than that in control groups (P < 0.05); TIMP-2 protein and mRNA expression in experimental group was significantly lower than that in control groups (P < 0.05); cell proliferation ability and invasiveness ability in experimental group were significantly higher than those in control groups (P < 0.05); LMVD-marked D2-40 and LMVD-marked LYVE-1 of transplanted tumor tissue in the experimental group were significantly higher than those in control groups (P < 0.05). The esophageal squamous carcinoma-related lymphatic microvessel could promote the proliferation and invasive ability of esophageal squamous carcinoma cells in vitro. It had different effects on esophageal carcinoma cells with different differentiation degree and had more obvious influence on poorly differentiated esophageal carcinoma cells, which may be related to the up-regulated MMP-2 expression and down-regulated TIMP-2 expression of esophageal carcinoma cells. The esophageal squamous carcinoma-related lymphatic microvessel endothelial cells could promote the growth of esophageal carcinoma-transplanted tumor of nude mice and lymphangiogenesis.

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Section: Discussionmentioning

confidence: 99%

Influence of lymphatic endothelial cells on proliferation and invasiveness of esophageal carcinoma cells in vitro and lymphangiogenesis in vivo

Nana

Sun

et al. 2015

Med Oncol

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“…These findings are not in agreement those reported by Kolben et al [111] and Shokry et al [112], who observed reduced immunologically defined MMP-9 levels in preeclamptic placental tissues collected at delivery. In addition, Galewska et al used distinct techniques to show that preeclamptic umbilical cord tissues (artery and vein) had lower MMP-2 and MMP-9 levels than healthy tissues [113]. However, they found increased MMP-9 levels in plasma obtained from umbilical cord blood samples from preeclamptic newborns, and no significant differences in MMP-2, TIMP-1 and TIMP-2 compared with those measured in healthy pregnancies [114].…”

Section: Clinical Findings Showing Mmp Alterations In Preeclampsiamentioning

confidence: 99%

Matrix Metalloproteinases as Drug Targets in Preeclampsia

Palei

Granger²,

Tanus‐Santos

2013

CDT

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Preeclampsia is an important syndrome complicating pregnancy. While the pathogenesis of preeclampsia is not entirely known, poor placental perfusion leading to widespread maternal endothelial dysfunction is accepted as a major mechanism. It has been suggested that altered placental expression of matrix metalloproteinases (MMPs) may cause shallow cytotrophoblastic invasion and incomplete remodeling of the spiral arteries. MMPs are also thought to link placental ischemia to the cardiovascular alterations of preeclampsia. In fact, MMPs may promote vasoconstriction and surface receptors cleavage affecting the vasculature. Therefore, the overall goal of this review article is to provide an overview of the pathophisiology of preeclampsia, more specifically regarding the role of MMPs in the pathogenesis of preeclampsia and the potential of MMP inhibitors as therapeutic options.

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“…Women with GH more often present a decrease in the umbilical cord length than women without it (13) . However, our data indicate that women with GH (82.8%) and GDM (82.1%) had a higher frequency of umbilical cord measuring between 36 and 70 cm.…”

Section: Discussionmentioning

confidence: 95%

Associação entre as características macroscópicas do cordão umbilical, gestação de alto risco e repercussões neonatais

Salge

Reis

Siqueira

et al. 2018

Rev. esc. enferm. USP

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Objective:To verify the association between the macroscopic characteristics of the umbilical cord, high-risk pregnancy and neonatal repercussions. Method: A crosssectional study carried out from January 2012 to January 2015 in a public maternity hospital in Goiânia/GO. The study population consisted of 126 recent puerperal women with diagnosis of high-risk pregnancy, and 139 clinically normal women (control group). Macroscopic features of the umbilical cord, maternal, fetal and neonatal diseases, gestational age, Apgar score, birth weight, head circumference and parity were evaluated. Data were descriptively analyzed. Results: 265 puerperal women and their respective newborns participated in the study. The most frequent characteristics of the umbilical cord of those with high-risk pregnancy and those from the control group were the absence of true knots (97.6% and 2.4%, respectively), length between 35 and 70 centimeters and paracentral insertion (81.7% and 18.3%). A statistical difference was observed between the high-risk pregnancy group and extremes of maternal age (p=0.004). Conclusion:The analysis and description of the characteristics of the umbilical cord carried out by the nurse lend important information about the neonatal prognosis. This evaluation subsidizes clinical practice and seeks to ensure the safety of the (mother-baby) binomial throughout the perinatal period. DESCRIPTORS INTRODUCTIONIn the gestational process, the line between normality and disease is extremely thin and the imbalance represents a high risk of maternal-fetal morbidity and mortality (1)(2) . About 20% of pregnancies have some type of complication (3) . Thus, women with alterations during pregnancy constitute a group known as "high-risk pregnant women"; a small number of pregnant women who have specific characteristics or because they suffer some injury are more likely to develop an unfavorable pregnancy (4) . In Brazil, high-risk pregnancies occur in 10% to 20% of the cases and are related to the occurrence of some disorders, the most frequent of which are gestational hypertension (GH) and gestational diabetes mellitus (GDM) (5) . Nearly 3/4 of maternal deaths in the world occur due to direct obstetric causes, and among them deaths due to hypertensive disorders represent about 14% (6) . There is a close relationship between placenta/umbilical cord, fetus and the change in its functions, as in the cases of GH and GDM. This relationship establishes different patterns of pregnancy outcomes and also the evolution of fetal and/or maternal injury (3) . The umbilical cord is an essential structure for maintenance of intrauterine life, and it can be used for assessing adverse perinatal conditions, thus contributing to a good neonatal prognosis (7) . This evaluation is performed through the macroscopic identification of umbilical cord alterations, and could, for example, elucidate causes of perinatal hypoxia which are not evidenced in the clinic, thus providing a better understanding of the physiopathogenesis of fetal events.I...

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Extracellular Matrix Remodeling of the Umbilical Cord in Pre-eclampsia as a Risk Factor for Fetal Hypertension

Cited by 18 publications

References 60 publications

Influence of lymphatic endothelial cells on proliferation and invasiveness of esophageal carcinoma cells in vitro and lymphangiogenesis in vivo

Influence of lymphatic endothelial cells on proliferation and invasiveness of esophageal carcinoma cells in vitro and lymphangiogenesis in vivo

Matrix Metalloproteinases as Drug Targets in Preeclampsia

Associação entre as características macroscópicas do cordão umbilical, gestação de alto risco e repercussões neonatais

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