Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse <i>plantaris</i> muscle

Stantzou, Amalia; Relizani, Karima; Morales‐Gonzalez, Susanne; Gallen, Charles; Grassin, Antoine; Ferry, Arnaud; Schuelke, Markus; Amthor, Helge

doi:10.1111/nan.12655

Cited by 15 publications

(12 citation statements)

References 67 publications

(96 reference statements)

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“…It could be speculated that enhanced postexercise extracellular matrix remodeling may be an important adaptation to facilitate the gains in muscle mass and/or maximal strength that occur following more prolonged resistance-type exercise training. In support, recent work demonstrates that enhanced extracellular matrix remodeling is associated with increases in muscle mass and strength in overloaded rodent plantaris muscle (Stantzou et al, 2020). Further exploration is required to determine whether the association between enhanced extracellular matrix remodeling and increases in muscle mass and strength following resistance-type exercise training in humans is causal.…”

Section: Discussionmentioning

confidence: 91%

Exercise Plus Presleep Protein Ingestion Increases Overnight Muscle Connective Tissue Protein Synthesis Rates in Healthy Older Men

Holwerda

Trommelen

Kouw

et al. 2021

International Journal of Sport Nutrition and Exercise Metabolism

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Protein ingestion and exercise stimulate myofibrillar protein synthesis rates. When combined, exercise further increases the postprandial rise in myofibrillar protein synthesis rates. It remains unclear whether protein ingestion with or without exercise also stimulates muscle connective tissue protein synthesis rates. The authors assessed the impact of presleep protein ingestion on overnight muscle connective tissue protein synthesis rates at rest and during recovery from resistance-type exercise in older men. Thirty-six healthy, older men were randomly assigned to ingest 40 g intrinsically L-[1-13C]-phenylalanine and L-[1-13C]-leucine-labeled casein protein (PRO, n = 12) or a nonprotein placebo (PLA, n = 12) before going to sleep. A third group performed a single bout of resistance-type exercise in the evening before ingesting 40 g intrinsically-labeled casein protein prior to sleep (EX+PRO, n = 12). Continuous intravenous infusions of L-[ring-2H5]-phenylalanine and L-[1-13C]-leucine were applied with blood and muscle tissue samples collected throughout overnight sleep. Presleep protein ingestion did not increase muscle connective tissue protein synthesis rates (0.049 ± 0.013 vs. 0.060 ± 0.024%/hr in PLA and PRO, respectively; p = .73). Exercise plus protein ingestion resulted in greater overnight muscle connective tissue protein synthesis rates (0.095 ± 0.022%/hr) when compared with PLA and PRO (p < .01). Exercise increased the incorporation of dietary protein-derived amino acids into muscle connective tissue protein (0.036 ± 0.013 vs. 0.054 ± 0.009 mole percent excess in PRO vs. EX+PRO, respectively; p < .01). In conclusion, resistance-type exercise plus presleep protein ingestion increases overnight muscle connective tissue protein synthesis rates in older men. Exercise enhances the utilization of dietary protein-derived amino acids as precursors for de novo muscle connective tissue protein synthesis during overnight sleep.

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Section: Discussionmentioning

confidence: 91%

Exercise Plus Presleep Protein Ingestion Increases Overnight Muscle Connective Tissue Protein Synthesis Rates in Healthy Older Men

Holwerda

Trommelen

Kouw

et al. 2021

International Journal of Sport Nutrition and Exercise Metabolism

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“…In support, findings from recent work demonstrated that enhanced ECM remodeling is associated with increases in muscle mass and strength in overloaded rodent plantaris muscle. 16 More exploration is required to determine whether the association between enhanced ECM remodeling and increases in muscle mass and strength following resistance-type exercise training in humans is both real and causal.…”

Section: The Impact Of Physical Activity On Collagen Remodelingmentioning

confidence: 99%

The impact of collagen protein ingestion on musculoskeletal connective tissue remodeling: a narrative review

Holwerda

Loon

2022

Nutrition Reviews

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Collagen is the central structural component of extracellular connective tissue, which provides elastic qualities to tissues. For skeletal muscle, extracellular connective tissue transmits contractile force to the tendons and bones. Connective tissue proteins are in a constant state of remodeling and have been shown to express a high level of plasticity. Dietary-protein ingestion increases muscle protein synthesis rates. High-quality, rapidly digestible proteins are generally considered the preferred protein source to maximally stimulate myofibrillar (contractile) protein synthesis rates. In contrast, recent evidence demonstrates that protein ingestion does not increase muscle connective tissue protein synthesis. The absence of an increase in muscle connective tissue protein synthesis after protein ingestion may be explained by insufficient provision of glycine and/or proline. Dietary collagen contains large amounts of glycine and proline and, therefore, has been proposed to provide the precursors required to facilitate connective tissue protein synthesis. This literature review provides a comprehensive evaluation of the current knowledge on the proposed benefits of dietary collagen consumption to stimulate connective tissue remodeling to improve health and functional performance.

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“…The ECM is an important source of growth factors, providing the attachment and controlled release of many growth factors and signal molecules, thereby aiding cell-to-cell signaling ( Birch, 2018 ). Transcriptome studies showed that in the rat model of overload, the expression abundance of the MSTN gene decreased, a large number of extracellular matrix-related proteins increased, and skeletal muscle increased ( Mendias et al, 2017 ; Stantzou et al, 2021 ). Biological scaffolds composed of ECM have been shown to facilitate the functional reconstruction of several tissue types including the esophagus ( Badylak et al, 2005 ; Desai et al, 2006 ), heart and vascular structures ( Badylak et al, 2006 ; Quarti et al, 2011 ), lower urinary tract ( Reddy et al, 2000 ; Boruch et al, 2010 ), and musculoskeletal tissues ( Smith et al, 2004 ; Franklin et al, 2008 ; Turner et al, 2010 ; Valentin et al, 2010 ; Zhao and Bass, 2018 ), among others.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development

et al. 2021

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Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to investigate the regulation mechanism of MSTN on the growth and development of bovine skeletal muscle. A total of 1,950 proteins were identified in MG.MSTN+/− and MG.WT. Compared with MG.WT cattle, a total of 320 differentially expressed proteins were identified in MG.MSTN cattle, including 245 up-regulated differentially expressed proteins and 75 down-regulated differentially expressed proteins. Bioinformatics analysis showed that knockdown of the MSTN gene increased the expression of extracellular matrix and ribosome-related proteins, induced activation of focal adhesion, PI3K-AKT, and Ribosomal pathways. The results of proteomic analysis were verified by muscle tissue Western blot test and in vitro MSTN gene knockdown test, and it was found that knockdown MSTN gene expression could promote the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells (BSMSCs). At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. These results suggest that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene. In general, this study provides new insights into the regulatory mechanism of MSTN involved in muscle growth and development.

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Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse plantaris muscle

Cited by 15 publications

References 67 publications

Exercise Plus Presleep Protein Ingestion Increases Overnight Muscle Connective Tissue Protein Synthesis Rates in Healthy Older Men

Exercise Plus Presleep Protein Ingestion Increases Overnight Muscle Connective Tissue Protein Synthesis Rates in Healthy Older Men

The impact of collagen protein ingestion on musculoskeletal connective tissue remodeling: a narrative review

Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development

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