Extracellular matrix surface network of embryogenic units of friable maize callus contains arabinogalactan-proteins recognized by monoclonal antibody JIM4

Šamaj, Jozef; Baluška, Frantǐsek; Bobák, M.; Volkmann, Dieter

doi:10.1007/s002990050588

Cited by 80 publications

(47 citation statements)

References 32 publications

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“…The formation of extracellular strands, fibrils and/or a continuous layer over callus cells accompanies the induction of morphogenesis in many plants cultured in vitro. This structure, which is referred to as extracellular matrix (ECM) or extracellular matrix surface network (ECMSN), has been found during the early stages of somatic embryogenesis and organogenesis e.g., in coconut [8] and maize [9]. The exact role of this structure is still not known, but its regulatory and coordinating functions during the early stages of morphogenesis have been suggested [8,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

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Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

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Section: Introductionmentioning

confidence: 99%

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Pilarska

Popielarska-Konieczna

Ślesak

et al. 2014

Acta Soc Bot Pol

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“…1 Šamaj et al, 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al, 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations.…”

mentioning

confidence: 99%

Are extracellular matrix surface network components involved in signalling and protective function?

Popielarska-Konieczna

Kozieradzka-Kiszkurno²,

Swierczynska³

et al. 2008

Plant Signaling & Behavior

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Endosperm is an interesting model for in vitro experiments, because of its unique origin, development and ploidy level. Here we used Actinidia deliciosa endosperm-derived callus to investigate morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM and TEM analysis showed that ECM is a heterogenous layer which consists of amorphous, dark-staining material, osmiophilic granules and reticulated fibres outside the outer callus cell wall. This structure may serve as a structural marker of morphogenic competence in endosperm-derived callus, because of its presence on the surface of callus forming morphogenic domains and its disappearance during organ growth. Based on immunolabelling, histochemistry, solvent and enzyme treatments, we suggest that pectins and lipids are components of the ECM layer. These results might indicate protective, water retention and/or cell communication functions for this ECM layer.In several plants cultured in vitro, SEM analysis revealed that induction of morphogenesis is linked to the appearance of a fibrillar network referred to as the extracellular matrix surface network (ECMSN). 1 Šamaj et al., 2,3 reported, that the ECMSN plays an important morphoregulatory role during somatic embryogenesis and organogenesis, implying an active role in plant morphogenesis. According to Bobák et al., 4 the chemical composition and structural arrangement of the ECMSN on the cell surface indicate that it may play a fundamental role in cell-to-cell recognition and interaction, cell division and differentiation, and also in generation and maintenance of some traits in plant cell populations. There is not a large body of data confirming the occurrence of ECM during in vitro organogenesis. 5,6 Most data concern the formation, structure and chemical composition and function of ECM in somatic and androgenic embryogenesis (reviewed in refs. 6-10). In previous histological and SEM analysis of morphogenic endosperm-derived callus of A. deliciosa, we described the presence of a membranous layer covering the callus surface, which we termed extracellular matrix (ECM). 11 The present experiments employed SEM, TEM and histochemical analysis to study the structure and composition of material coating the callus surface.SEM revealed the presence of heterogeneous material covering the callus surface. A smooth membranous layer coated some parts of the callus surface (Fig. 1A), while other regions were covered by fibrillar structures and granules of mucilage-like secretion forming a network at site of cell-cell adhesion. Similar structures were observed during in vitro induction of somatic embryogenesis, androgenesis and organogenesis in different species (reviewed in refs. 7-13).TEM showed a layer composed of amorphous material with fibrillar and spherical components covering morphogenic cell clusters. The cell wall of non-embryogenic and senescent cells was also covered with ECM, but it differed in appearance: the layer was granular, with amo...

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“…[8][9][10][11][12] Also, the report of an extracellular matrix connecting cell wall-plasmalemma-cytoskeleton led to the hypothesis that the cell wall could be sensitive to osmotic stresses. [13][14][15][16] Supporting this possibility, several groups reported that treatments with plasmolysing agents led to increase the ISE of gymnosperms and Eleutherococcus. 17,18 Numerous studies have reported that soluble molecules are released in the medium during the acquisition of totipotency.…”

Section: Introductionmentioning

confidence: 89%

“…34,43,44 Exogenous addition of ABA led to improve the ISE in monocots (Cocos nucifera, Cynodon hybrid Musa sp.) 9,11,13 and in dicots (Daucus carota, Hevea). 8,10,12 Our experiment in chicory established that 50 mM ABA promoted the ISE whereas 100 mM had a slightly negative effect.…”

Section: Discussionmentioning

confidence: 99%

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Couillerot

Windels

Vazquez

et al. 2012

Plant Signaling & Behavior

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Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the C. intybus cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.

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Extracellular matrix surface network of embryogenic units of friable maize callus contains arabinogalactan-proteins recognized by monoclonal antibody JIM4

Cited by 80 publications

References 32 publications

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Are extracellular matrix surface network components involved in signalling and protective function?

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

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