Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

Rehbein, Grit; Schmidt, Bernd; Fleischhacker, Michael

doi:10.1016/j.cca.2015.07.027

Cited by 20 publications

(17 citation statements)

References 49 publications

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“…Conversely, no data have been thus far reported in thyroid tumors for miR-148b, miR-1285-5p and miR-4516, although deregulation of these miRNAs is associated with other cancer types. [50][51][52][53][54] Taken together, our data suggest that evaluating miR-146-5p, miR-199a, miR199b, miR-1285-5p, miR-1915-3p, miR-4516, and miR-148b could be a useful diagnostic tool for differentiating between encapsulated tumors and other more aggressive entities.…”

Section: Discussionmentioning

confidence: 81%

miRNA expression profiling of ‘noninvasive follicular thyroid neoplasms with papillary-like nuclear features' compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas

et al. 2017

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Follicular variants of papillary thyroid carcinoma include encapsulated (with or without capsular/vascular invasion) and infiltrative forms, which have different clinical behaviors. The encapsulated forms that lack capsular invasion have an indolent clinical behavior that is similar to benign lesions; therefore, they were recently reclassified as 'noninvasive follicular thyroid neoplasms with papillary-like nuclear features' (NIFTPs). Because NIFTPs have nuclear features of papillary carcinomas, distinguishing between NIFTPs and infiltrative follicular variant of papillary thyroid carcinoma is almost impossible with cytological examination. The aim of this study is to determine whether miRNA expression profiles may help distinguish between NIFTPs versus follicular adenomas and infiltrative follicular variant of papillary thyroid carcinomas. The expression profiling of 798 miRNAs was tested in 54 thyroid tumors, including 18 follicular adenomas, 19 NIFTPs and 17 infiltrative follicular variant of papillary thyroid carcinomas, using nCounter Nanostring. We found that miR-146-5p, miR-221-5p, miR-222-3p, miR-30e-3p, and miR-152-3p could discriminate between benign and malignant lesions with a very high level of significance (P-value<0.001). High expression levels of miR-146-5p, miR-199a-5p, miR-199b-5p, miR-1285-5p, miR-1915-3p, and miR-4516, and low miR-148b-3p expression were associated with infiltrative growth of follicular variant of papillary thyroid carcinomas. Interestingly, miR-152-3p, miR-185-5p, and miR-574-3p were significantly downregulated in NIFTPs compared with follicular adenomas, whereas miR-10a-5p and miR-320e can discriminate between NIFTPs and infiltrative forms of follicular variant of papillary thyroid carcinomas. In conclusion, a panel of these markers could have high diagnostic potential as well as could be applied to presurgical fine-needle aspiration, especially for lesions classified as indeterminate thyroid nodules.

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Section: Discussionmentioning

confidence: 81%

miRNA expression profiling of ‘noninvasive follicular thyroid neoplasms with papillary-like nuclear features' compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas

et al. 2017

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“…Es gibt Hinweise darauf, dass Lungenkrebspatienten auffallende microRNA-Muster in Sputum und BAL aufweisen, sodass deren Bestimmung möglicherweise in der Tumornachsorge eine Bedeutung bekommen könnte [12]. Dafür sind jedoch weitere prospektive Studien, insbesondere in der Tumornachsorge, sinnvoll.…”

Section: Resultsunclassified

Evaluation zum möglichen Stellenwert der Bronchiallavagezytologie bei Erstdiagnose und Nachsorge des Lungenkarzinoms

Grünewaldt

Hügel

Hermann³

et al. 2017

Pneumologie

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Bronchoalveolar lavage [BAL] is an important procedure in the diagnosis of a variety of lung diseases. While it has enormous value in the diagnostics of inflammatory parenchymal diseases, its significance in lung cancer is unclear. Keeping in mind that immune therapy (e. g. application of checkpoint inhibitors) is gaining importance in the management of lung carcinoma, it is important to know if there are typical cellular patterns in BAL of lung cancer patients. In a retrospective proof of principle-study, we analyzed 38 patients who underwent BAL at the initial diagnosis of lung cancer. We observed an elevated level of CD25 lymphocytes as well as an increased expression of DR antigen, both signaling lymphocyte activation. We could not find a typical cytologic pattern of inflammatory cells in lung carcinoma patients. Sensitivity of BAL to malignant cells was rare, thus confirming earlier analysis. We could not demonstrate typical cellular patterns in BAL of lung cancer patients. Evaluation of specific microRNA patterns might play a supporting role in the initial diagnosis as well as follow-up of lung cancer patients.

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“…MiRNAs are a class of small noncoding RNAs. There are several researches nd that miRNAs are associated with a wide range of cellular processes, such as cellular proliferation, differentiation, apoptosis, and play a critical role in cancer [24][25][26]. Recently, miR-449a as a member of miRNAs has been found down-regulated in several types of cancers including prostate cancer, gastric cancer, bladder cancer as well as lung cancer [17,19,[27][28][29].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-449a Plays an Indicative Role in Diagnosing Lung Cancer

Yang

Zhong

Yang

et al. 2020

Preprint

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Background: Lung cancer is one of the most common causes of cancer death among all the malignancies worldwide. Evidences suggest that the incidence and mortality of lung cancer has been on the rise. MicroRNA-449a (miR-449a) as one important member of microRNAs, has been demonstrated acting as a tumor suppressor in lung cancer. In this study, we sought to assess the relationship between miR-449a expression level and diagnostic value of lung cancer.Methods: In this present research, quantitative Real-Time PCR was applied to detect the miR-449a expression in 116 lung cancer patients and 41 healthy volunteers. The diagnostic value of miR-449a in lung cancer patients was determined by receiver operating characteristic (ROC) curve.Results: MiR-449a was significantly down-regulated in lung cancer patients compared with healthy control (P<0.05). In addition, miR-449a expression was associated with sex (P=0.004), tumor size (P=0.000), TNM stage (P=0.006) and metastasis (P=0.036). However, there was no correlation with age, smoking history and histological type of lung cancer patients (all P＞0.05). In the ROC analysis, the results showed that the area under the ROC curve (AUC) was 0.902 with the sensitivity of 94.8% and specificity of 78.0%, and the optimum cutoff value was 2.255.Conclusion: MiR-449a expression was down-regulated in lung cancer patients, and it could be an efficient diagnostic biomarker in lung cancer patients.

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Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

Cited by 20 publications

References 49 publications

miRNA expression profiling of ‘noninvasive follicular thyroid neoplasms with papillary-like nuclear features' compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas

miRNA expression profiling of ‘noninvasive follicular thyroid neoplasms with papillary-like nuclear features' compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas

Evaluation zum möglichen Stellenwert der Bronchiallavagezytologie bei Erstdiagnose und Nachsorge des Lungenkarzinoms

MicroRNA-449a Plays an Indicative Role in Diagnosing Lung Cancer

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