Extracellular potassium concentrations in red blood cell suspensions after irradiation and washing

Weiskopf, Richard B.; Schnapp, Stephanie; Rouine‐Rapp, Kathryn; Bostrom, Alan; Toy, Pearl

doi:10.1111/j.1537-2995.2005.00220.x

Cited by 63 publications

(67 citation statements)

References 27 publications

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“…On the other hand, the changes between pre and post-irradiation in each group of up to 14 days storage and >14 days storage showed significant changes in the plasma haemoglobin indicates irradiation itself also causes some changes (table-3). This study showed similarity with previous study where free haemoglobin levels were increased in stored irradiated red cell components (Weiskopf et al, 2005) 19 but remain within acceptable limits. Regarding the biochemical values of Na + , K + and LDH, comparison at different storage period of 14 days and >14 days showed significant changes in Na + , K + and LDH which reflects gradual storage changes in both pre and post irradiated samples.…”

Section: Methodssupporting

confidence: 91%

Effects of gamma irradiation on red blood cells at different storage period: A comparative study

Yousuf

Mobin

Subramaniam

et al. 2018

Bangladesh J Med Sci

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Objectives: The objective of this study was to explore the effect of gamma irradiation on the haematological and biochemical parameters of the allogeneic red blood cells units at different storage duration. Methods: A prospective comparative study was conducted at the Blood Bank unit, Department of Diagnostic Laboratory Services of Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in year 2013. Total 36 allogeneic red blood cell units were selected by simple random sampling method and divided into two groups, 18 units in each group based on the storage period into i) up to 14 days and ii) >14 days to expiry. Pre and post irradiation samples were taken immediately before and after irradiation of the units to analyse for the haematological parameters such as plasma Hb level, percent red cell haemolysis, haemoglobin per unit blood (Hb/unit), haematocrit (HCT), red cell indices such as mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and biochemical parameters such as plasma sodium (Na + ), potassium (K + ) and LDH levels. Data was collected and statistical analysis was done. Results: Statistically significant changes were observed between red cell units of up to 14 days and > 14 days old and pre irradiated samples in terms of plasma haemoglobin level, percent red cell haemolysis and plasma Na + , K + and LDH levels. However, pre and post irradiation samples in each group of 14 days and >14 days showed significant changes in the plasma Hb and % haemolysis, Hb/unit, HCT, MCV and MCH in 14 days group while only in plasma Hb, MCV and MCH in >14 days group without any effect on plasma Na + , K + and LDH levels in both group. Conclusion: Comparison at two different red cells storage groups i.e. period of up to 14 days and >14 days, showed significant changes in haematological and biochemical parameters. In the pre irradiation group this reflects the gradual storage changes over time even without going through the irradiation process. On the other hand, the changes between pre and post-irradiation for both the groups of storage showed significant haematological changes indicating irradiation itself also causes significant changes. In view of all these findings, we recommend to irradiate the allogeneic red cell units within 14 days of collection to reduce the adverse effects resulted from irradiation on the cellular products.

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Section: Methodssupporting

confidence: 91%

Effects of gamma irradiation on red blood cells at different storage period: A comparative study

Yousuf

Mobin

Subramaniam

et al. 2018

Bangladesh J Med Sci

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“…This latter rationale has been challenged, particularly when applied to less-than-massive volumes of blood transfused, because when small volumes are transfused, the major consideration for the patient is the total amount of K þ transfused and the transfusion rate. 12,13 During ECMO, the transfusion volume is sufficiently small and the blood is infused sufficiently slow that it probably completely mixes with the patient's blood volume, resulting in little, if any, increase of in vivo plasma K þ levels.…”

Section: Discussionmentioning

confidence: 99%

Increased hemolysis from saline pre-washing RBCs or centrifugal pumps in neonatal ECMO

et al. 2007

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Objectives: Hemolysis is a significant complication of extracorporeal membrane oxygenation (ECMO), with a reported incidence of 12.2%. The aims of this study were (1) to investigate hemolysis caused by saline-washed versus unwashed RBCs, (2) to determine in vitro the effects of saline washing on erythrocyte hemolytic markers and (3) to investigate hemolysis by centrifugal versus roller pumps.Study design: (1) To evaluate the effect of pre-transfusion saline-washing versus non-washing, the peak plasma-free hemoglobin (FHb) and total bilirubin in the first 3 days versus the next 4 days of ECMO were compared (2) Pre-and postsaline-washed RBCs were analyzed for K þ , hemoglobin, mean corpuscular volume, FHb and hemolysis at baseline and after 4 h of storage at 41C. (3) Over 10 000 neonatal ECMO cases were retrospectively reviewed to study the effect of pump type on hemolysis.Results: (1) The washed blood group had significantly more hemolysis within the first 3 days of ECMO. (2) Immediately after saline washing, the K þ and Hb concentrations were significantly decreased compared with unwashed blood, and these differences were maintained after 4 h. The osmotic fragility of washed RBCs after 4 h of storage at 41C was significantly higher than at baseline. (3) Hemolysis was reported more often in the centrifugal than in the roller pump group.Conclusions: (1) Using unwashed RBCs decreased hemolysis within the first 3 days of ECMO. (2) Saline washing, while decreasing the concentration of K þ in the plasma, significantly increases RBC membrane osmotic fragility. (3) Hemolysis is linked to the use of centrifugal pumps.

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“…[2][3][4][5] Evidence for such damage includes impaired aggregability, adherence, and deformability, 6 enhanced haemoglobin and glutathione (GSH) oxidation, 7 increased haemolysis and leakage of intracellular potassium, and lactate dehydrogenase. [8][9][10][11] Plant compounds can protect cells against radiationinduced ROS-mediated damage. [12][13][14] Quercetin, 3, 3 ′ , 4 ′ , 5, 7-pentahydroxyflavone, is one of the most abundant dietary polyphenolic substances occurring in fruits and vegetables.…”

Section: Introductionmentioning

confidence: 99%

Does quercetin protect human red blood cell membranes against γ-irradiation?

et al. 2013

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Extracellular potassium concentrations in red blood cell suspensions after irradiation and washing

Cited by 63 publications

References 27 publications

Effects of gamma irradiation on red blood cells at different storage period: A comparative study

Effects of gamma irradiation on red blood cells at different storage period: A comparative study

Increased hemolysis from saline pre-washing RBCs or centrifugal pumps in neonatal ECMO

Does quercetin protect human red blood cell membranes against γ-irradiation?

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