1987
DOI: 10.1016/0168-1656(87)90014-9
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Extracellular production of human growth hormone by a head portion of the prepropeptide derived from Bacillus amyloliquefaciens neutral protease in Bacillus subtilis

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Cited by 14 publications
(2 citation statements)
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“…Salting-out processes, with ammonium sulfate in particular, have been in wide use since that time; Englard and Seifter (1990) have estimated that up to 80% of published protein purification protocols involve at least one salting-out step. The use of precipitation ranges from primary isolation procedures (Nakayama et al, 1987;Michaels, 1990) for the separation of product protein from cell debris in recombinant cell cultures, to finishing operations (Bell et al, 1983;Paul and Rosas. 1990) for the production of solids prior to drying and formulation.…”
mentioning
confidence: 99%
“…Salting-out processes, with ammonium sulfate in particular, have been in wide use since that time; Englard and Seifter (1990) have estimated that up to 80% of published protein purification protocols involve at least one salting-out step. The use of precipitation ranges from primary isolation procedures (Nakayama et al, 1987;Michaels, 1990) for the separation of product protein from cell debris in recombinant cell cultures, to finishing operations (Bell et al, 1983;Paul and Rosas. 1990) for the production of solids prior to drying and formulation.…”
mentioning
confidence: 99%
“…The efficiency of chromatographic separations is largely dependent on upstream precipitation procedures (Englard & Seifer, 1990). Up to 80% of published protein purification protocols include at least one precipitation step, ranging from primary isolation [separating cellular debris from protein product in recombinant cell cultures (McGregor, 1983;Nakayama et al, 1987)] to finishing operations [producing solids prior to drying and formulation (Hoare et al, 1983;Paul & Rosas, 1990)], to yield a partially purified product of reduced volume (Chen et al, 1992;Englard & Seifer, 1990). Precipitation processes also rapidly isolate protein products, reducing their exposure to denaturing conditions and proteolytic enzymes (Chen et al, 1992).…”
Section: Introductionmentioning
confidence: 99%