Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

Thiribhuvanamala, G.; Kalaiselvi, G.; Parthasarathy, S.; Madhavan, S.; Prakasam, V.

doi:10.21276/ap.2017.6.1.4

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…The enzyme cocktail that was obtained after 4 days of fungal growth on hay as a carbon source, in subsequent hay hydrolysis demonstrated 63% conversion rate into reducing sugars. These observations to some extent contradict with other studies where white-rot fungi produced the most active enzymes after 12 days of incubation [ 21 ]. The differences could be explained by previous assessment of only individual enzymes or their limited combinations [ 8 , 21 ] or the by more intense binding of the enzyme to carbohydrate source (hay) at later incubation stage and inefficient recovery of that enzyme fraction.…”

Section: Resultscontrasting

confidence: 99%

“…These observations to some extent contradict with other studies where white-rot fungi produced the most active enzymes after 12 days of incubation [ 21 ]. The differences could be explained by previous assessment of only individual enzymes or their limited combinations [ 8 , 21 ] or the by more intense binding of the enzyme to carbohydrate source (hay) at later incubation stage and inefficient recovery of that enzyme fraction. In this study, the performance of the whole crude enzyme cocktail without purification and removal of any specific protein fraction was assessed.…”

Section: Resultscontrasting

confidence: 99%

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Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application

Mežule

Civzele

2020

JoF

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Lignocellulosic biomass can be used as a source for energy, fuel and valuable chemical production. From all available technologies, biological approaches have been recognized as the most environmentally friendly and sustainable ones. At the same time, high conversion costs, low efficiency and environmental issues still hinder the introduction of biological processes into industrial scale manufacturing. The aim of this study was to determine the most suitable enzyme cocktail recovery conditions from a biomass–fungal culture of the white-rot basidiomycete Irpex lacteus. Subsequent evaluation of the overall enzyme cocktail efficiency to release fermentable carbohydrates from biomass showed that prolonged fungal cultivation decreases the quality of the produced enzyme cocktail. At the same time, introduction of ultrasound pre-treatment during enzyme extraction improved the recovered enzyme cocktail efficiency in converting biomass to fermentable sugars, yielding up to 0.25 g of fermentable sugar per g dry hay biomass and up to 0.11 g per g dried straw or microalgae substrates. The results demonstrated that the production of lignocellulose-degrading enzymes from fungi is more sensitive than previously described, especially in terms of fungal growth, culture sterility and incubation conditions.

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Section: Resultscontrasting

confidence: 99%

Section: Resultscontrasting

confidence: 99%

Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application

Mežule

Civzele

2020

JoF

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“…Our results coincide with reports of compounds derived from agro-industrial residues, such as wheat straw, rice straw, corn stubble, and cane bagasse, functioning as inducers of the main lignin-modifying enzymes in white rot fungi [64][65][66]. Furthermore, the addition of dyes had a clear induction effect on enzymatic activity [67,68].…”

Section: Discussionsupporting

confidence: 90%

Isolation of Fungi from a Textile Industry Effluent and the Screening of Their Potential to Degrade Industrial Dyes

Juárez-Hernández

Castillo-Hernández

Pérez-Parada

et al. 2021

JoF

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Six fungal strains were isolated from the textile industry effluent in which they naturally occur. Subsequently, the fungal strains were identified and characterized in order to establish their potential decolorizing effect on textile industry effluents. The strains of interest were selected based on their capacity to decolorize azo, indigo, and anthraquinone dyes. Three of the strains were identified as Emmia latemarginata (MAP03, MAP04, and MAP05) and the other three as Mucor circinelloides (MAP01, MAP02, and MAP06), while the efficiency of their decolorization of the dyes was determined on agar plate and in liquid fermentation. All the strains co-metabolized the dyes of interest, generating different levels of dye decolorization. Plate screening for lignin-degrading enzymes showed that the MAP03, MAP04, and MAP05 strains were positive for laccase and the MAP01, MAP02, and MAP06 strains for tyrosinase, while all strains were positive for peroxidase. Based on its decolorization capacity, the Emmia latemarginata (MAP03) strain was selected for the further characterization of its growth kinetics and ligninolytic enzyme production in submerged fermentation under both enzyme induction conditions, involving the addition of Acetyl yellow G (AYG) dye or wheat straw extract, and no-induction condition. The induction conditions promoted a clear inductive effect in all of the ligninolytic enzymes analyzed. The highest level of induced enzyme production was observed with the AYG dye fermentation, corresponding to versatile peroxidase (VP), manganese peroxidase (MnP), and lignin peroxidase (LiP). The present study can be considered the first analysis of the ligninolytic enzyme system of Emmia latemarginata in submerged fermentation under different conditions. Depending on the results of further research, the fungal strains analyzed in the present research may be candidates for further biotechnological research on the decontamination of industrial effluents.

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“…In contrast to the findings presented here, they did not observe the significant influence of glucose or glycerol on laccase production. Various reports have indicated that the production of LMEs in basidiomycetes is dependent on the carbon and nitrogen sources used, as well as the presence of aromatic compounds in the culture medium ( Elisashvili, Kachlishvili & Asatiani, 2018 ; Stajić et al, 2006 ; Thiribhuvanamala et al, 2017 ). For instance, Elisashvili et al (2002) demonstrated the effect of different carbon sources and aromatic compounds on the lignocellulolytic enzyme activity of different edible and medicinal basidiomycetes, concluding that it is possible not only to increase lignocellulolytic activity substantially, but also to trigger their preferential synthesis by supplementing the culture medium with nutritional compounds.…”

Section: Discussionmentioning

confidence: 99%

Regulation of dye-decolorizing peroxidase gene expression in Pleurotus ostreatus grown on glycerol as the carbon source

Cuamatzi-Flores,

Nava-Galicia,

Esquivel-Naranjo

et al. 2024

PeerJ

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Dye-decolorizing peroxidases (DyPs) (E.C. 1.11.1.19) are heme peroxidases that catalyze oxygen transfer reactions similarly to oxygenases. DyPs utilize hydrogen peroxide (H2O2) both as an electron acceptor co-substrate and as an electron donor when oxidized to their respective radicals. The production of both DyPs and lignin-modifying enzymes (LMEs) is regulated by the carbon source, although less readily metabolizable carbon sources do improve LME production. The present study analyzed the effect of glycerol on Pleurotus ostreatus growth, total DyP activity, and the expression of three Pleos-dyp genes (Pleos-dyp1, Pleos-dyp2 and Pleos-dyp4), via real-time RT-qPCR, monitoring the time course of P. ostreatus cultures supplemented with either glycerol or glucose and Acetyl Yellow G (AYG) dye. The results obtained indicate that glycerol negatively affects P. ostreatus growth, giving a biomass production of 5.31 and 5.62 g/L with respective growth rates (micra; m) of 0.027 and 0.023 h−1 for fermentations in the absence and presence of AYG dye. In contrast, respective biomass production levels of 7.09 and 7.20 g/L and growth rates (μ) of 0.033 and 0.047 h−1 were observed in equivalent control fermentations conducted with glucose in the absence and presence of AYG dye. Higher DyP activity levels, 4,043 and 4,902 IU/L, were obtained for fermentations conducted on glycerol, equivalent to 2.6-fold and 3.16-fold higher than the activity observed when glucose is used as the carbon source. The differential regulation of the DyP-encoding genes in P. ostreatus were explored, evaluating the carbon source, the growth phase, and the influence of the dye. The global analysis of the expression patterns throughout the fermentation showed the up- and down- regulation of the three Pleos-dyp genes evaluated. The highest induction observed for the control media was that found for the Pleos-dyp1 gene, which is equivalent to an 11.1-fold increase in relative expression (log2) during the stationary phase of the culture (360 h), and for the glucose/AYG media was Pleos-dyp-4 with 8.28-fold increase after 168 h. In addition, glycerol preferentially induced the Pleos-dyp1 and Pleos-dyp2 genes, leading to respective 11.61 and 4.28-fold increases after 144 h. After 360 and 504 h of culture, 12.86 and 4.02-fold increases were observed in the induction levels presented by Pleos-dyp1 and Pleos-dyp2, respectively, in the presence of AYG. When transcription levels were referred to those found in the control media, adding AYG led to up-regulation of the three dyp genes throughout the fermentation. Contrary to the fermentation with glycerol, where up- and down-regulation was observed. The present study is the first report describing the effect of a less-metabolizable carbon source, such as glycerol, on the differential expression of DyP-encoding genes and their corresponding activity.

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Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

Cited by 8 publications

References 24 publications

Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application

Bioprospecting White-Rot Basidiomycete Irpex lacteus for Improved Extraction of Lignocellulose-Degrading Enzymes and Their Further Application

Isolation of Fungi from a Textile Industry Effluent and the Screening of Their Potential to Degrade Industrial Dyes

Regulation of dye-decolorizing peroxidase gene expression in Pleurotus ostreatus grown on glycerol as the carbon source

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