Extracellular single-unit recordings from peripheral nerve axons in vitro by a novel multichannel microelectrode array

Guo, Tiantian; Chen, Longtu; Tran, Khanh T. M.; Ghelich, Pejman; Guo, Yi-Syuan; Nolta, Nicholas F.; Emadi, Sharareh; Han, Martin; Feng, Bin

doi:10.1016/j.snb.2020.128111

Cited by 15 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recording neural activity from autonomic nerves is very challenging due to the often sub-millimeter nature of these nerves 23 , the protective layers surrounding the nerve (epineurium), bundle of axons (perineurium), and individual axons (endoneurium) 24 , 25 , and the low-amplitude waveforms generated from small unmyelinated C-fibers 26 that dominate autonomic nerves 27 , 28 . Studies have applied electrical stimulation on autonomic nerves to record evoked neural activity using extraneural electrodes, which record from outside the nerve 9 , 29 , and intraneural electrodes, which penetrate the nerve 30 .…”

Section: Introductionmentioning

confidence: 99%

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Jiman

Ratze

Welle

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Autonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8–9 µm in diameter, 150–250 µm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1–91.7 µV and signal-to-noise ratios of 2.0–8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7–4.4 m/s) and efferent (0.7–8.8 m/s) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.

show abstract

Section: Introductionmentioning

confidence: 99%

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Jiman

Ratze

Welle

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Recording neural activity from autonomic nerves is very challenging due to the often submillimeter nature of these nerves 23 , the protective layers surrounding the nerve (epineurium), bundle of axons (perineurium) and individual axons (endoneurium) 24,25 , and the low-amplitude waveforms generated from small unmyelinated C-fibers 26 that dominate autonomic nerves 27,28 .…”

Section: Introductionmentioning

confidence: 99%

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Jiman

Ratze

Welle

et al. 2020

Preprint

View full text Add to dashboard Cite

25Autonomic nerves convey essential neural signals that regulate vital body functions. Recording 26 clearly distinctive physiological neural signals from autonomic nerves will help develop new 27 treatments for restoring regulatory functions. However, this is very challenging due to the small 28 nature of autonomic nerves and the low-amplitude signals from their small axons. We developed 29 a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-30 small electrodes (8-9 µm in diameter, 150-250 µm in length) for recording physiological action 31 potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 32 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We 33 recorded action potentials with peak-to-peak amplitudes of 15.1-91.7 µV and signal-to-noise 34 ratios of 2.0-8.3 on multiple carbon fibers per experiment, determined conduction velocities of 35 some vagal signals in the afferent (0.7-1.0 m/sec) and efferent (0.7-8.8 m/sec) directions, and 36 monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, 37 these experiments demonstrated that CFMAs are a novel interface for in-vivo intraneural action 38 potential recordings from autonomic nerves. This work is a milestone towards the 39 comprehensive understanding of physiological neural signaling and the development of 40 innovative treatment modalities for restoring vital functions controlled by autonomic nerves. 41 42

show abstract

“…Although a powerful method, single-fiber recordings have been used only by a few studies to assess neuromodulatory effects 6,28 because of the challenges of recording single units from peripheral axons. 10 Recent advances in microelectrode arrays interfacing with peripheral nerves 26 will likely allow a broader application of single-fiber recordings in neuromodulation research.…”

Section: Discussionmentioning

confidence: 99%

Blocking peripheral drive from colorectal afferents by subkilohertz dorsal root ganglion stimulation

Chen

Guo

Zhang

et al. 2021

Pain

Self Cite

View full text Add to dashboard Cite

Clinical evidence indicates dorsal root ganglion (DRG) stimulation effectively reduces pain without the need to evoke paresthesia. This paresthesia-free anesthesia by DRG stimulation can be promising to treat pain from the viscera, where paresthesia usually cannot be produced. Here, we explored the mechanisms and parameters for DRG stimulation using an ex vivo preparation with mouse distal colon and rectum (colorectum), pelvic nerve, L6 DRG, and dorsal root in continuity. We conducted single-fiber recordings from split dorsal root filaments and assessed the effect of DRG stimulation on afferent neural transmission. We determined the optimal stimulus pulse width by measuring the chronaxies of DRG stimulation to be below 216 ms, indicating spike initiation likely at attached axons rather than somata. Subkilohertz DRG stimulation significantly attenuates colorectal afferent transmission (10, 50, 100, 500, and 1000 Hz), of which 50 and 100 Hz show superior blocking effects. Synchronized spinal nerve and DRG stimulation reveals a progressive increase in conduction delay by DRG stimulation, suggesting activity-dependent slowing in blocked fibers. Afferents blocked by DRG stimulation show a greater increase in conduction delay than the unblocked counterparts. Midrange frequencies (50-500 Hz) are more efficient at blocking transmission than lower or higher frequencies. In addition, DRG stimulation at 50 and 100 Hz significantly attenuates in vivo visceromotor responses to noxious colorectal balloon distension. This reversible conduction block in C-type and Ad-type afferents by subkilohertz DRG stimulation likely underlies the paresthesia-free anesthesia by DRG stimulation, thereby offering a promising new approach for managing chronic visceral pain.

show abstract

Extracellular single-unit recordings from peripheral nerve axons in vitro by a novel multichannel microelectrode array

Cited by 15 publications

References 42 publications

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

Blocking peripheral drive from colorectal afferents by subkilohertz dorsal root ganglion stimulation

Contact Info

Product

Resources

About