Extracellular Synthesis of Bactericidal Silver Nanoparticles Using Actinobacterium Dagang 5

Abstract

Abstract:Biologically synthesised silver nanoparticle using microbes are potential, cost effective and sustainable. Basically actinomycetes are popular for antibiotic and other therapeutic needs. In the present study the silver nanoparticle was synthesised using Actinobacteriumdagang 5 from marine sediments. The silver nitrate was treated with cell free supernatant of Actinobacteriumdagang 5 to synthesis the bactericidal silver nanoparticle and the initial characterisation was performed by observation of color change to intense brown color, UV-Vis spectrum of nanoparticle exhibited an absorption peak at 420nm that indicate surface Plasmon resonance of nanoparticles, X-Ray Diffraction (XRD) patterns showed the distinct peaks, and confirmsthat the nanoparticles are crystalline in nature at average size of 39.28nm. Fourier Transform Infrared Spectroscopy (FT-IR) analysis reveals the evidence, the protein acts as reducing and capping agents. Field Emission -Scanning Electron Microscope (FE-SEM) images showed the formation of nanoparticle. Energy Dispersive X-ray (EDAX) spectra register the presence of silver by showing as major signal. The stability of synthesised nanoparticle was analysed, the zeta potential of our nanoparticle is -21.3mV, which showed nanoparticle are highly stable. The biologically synthesised silver nanoparticle exemplified potential bactericidal efficacy towards the bacterial pathogens. From this study connote that Actinobacteriumdagang 5 is more desirable for the synthesis of Silver nanoparticle by extracellular method in the mode of efficient and eco friendly.