Extracellular vesicle-associated proteins as potential biomarkers

Schou, Anne Sophie; Nielsen, Jonas Ellegaard; Askeland, Anders; Jørgensen, Maléne Møller

doi:10.1016/bs.acc.2020.02.011

Cited by 10 publications

(7 citation statements)

References 172 publications

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“…EVs play important roles in both immune activation and tolerance, and are involved in many diseases such as cancer, making them both attractive targets and vehicles in therapy (Karlsson et al., 2001 ; Markov et al., 2019 ; Pascucci et al., 2014 ; Peinado et al., 2012 ; Raposo et al., 1996 ; Zitvogel et al., 1998 ). In addition, EVs released during disease can carry disease specific markers making them of great interest and consequently they have an emerging role as biomarkers (Boukouris & Mathivanan, 2015 ; Danzer et al., 2012 ; Eldh et al., 2014 ; Hurwitz et al., 2016 ; Logozzi et al., 2009 ; Schou et al., 2020 ; Skog et al., 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

Veerman

Teeuwen

Czarnewski

et al. 2021

J of Extracellular Vesicle

198

150

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Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers, but still EV subtypes are not fully characterised. To isolate EVs with few co-isolated entities, a combination of methods is needed. However, this is timeconsuming and requires large sample volumes, often not feasible in most clinical studies or in studies where small sample volumes are available. Therefore, we compared EVs rendered by five commonly used methods based on different principles from conditioned cell medium and 250 µl or 3 ml plasma, that is, precipitation (Exo-Quick ULTRA), membrane affinity (exoEasy Maxi Kit), size-exclusion chromatography (qEVoriginal), iodixanol gradient (OptiPrep), and phosphatidylserine affinity (MagCapture). EVs were characterised by electron microscopy, Nanoparticle Tracking Analysis, Bioanalyzer, flow cytometry, and LC-MS/MS. The different methods yielded samples of different morphology, particle size, and proteomic profile. For the conditioned medium, Izon 35 isolated the highest number of EV proteins followed by exoEasy, which also isolated fewer non-EV proteins. For the plasma samples, exoEasy isolated a high number of EV proteins and few non-EV proteins, while Izon 70 isolated the most EV proteins. We conclude that no method is perfect for all studies, rather, different methods are suited depending on sample type and interest in EV subtype, in addition to sample volume and budget.

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Section: Introductionmentioning

confidence: 99%

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

Veerman

Teeuwen

Czarnewski

et al. 2021

J of Extracellular Vesicle

198

150

View full text Add to dashboard Cite

show abstract

“…To date, the application potential of EVs is mainly in the diagnosis and treatment of diseases. Based on the analysis of EVs, cell-specific or diseasespecific proteins can be identified, thereby contributing to disease diagnosis (Schou et al, 2020). The therapeutic potential of EVs, on the one hand, is as a vehicle for drug delivery (Kalluri and LeBleu, 2020), and on the other hand, the specific cell-derived EVs themselves have therapeutic effects.…”

Section: Introductionmentioning

confidence: 99%

An Overview of Current Research on Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Bibliometric Analysis From 2009 to 2021

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Background: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are important mediators of intercellular communication and participate in numerous physiological and pathological processes in the body. This study aims to introduce the research status, analyze the research hotspots, and predict the development trend through bibliometric analysis of MSC-EVs.Methods: We searched all relevant literature on MSC-EVs from 2009 to 2021 in the Web of Science. R-bibliometrix, VOSviewer, and CiteSpace software were used to visualize the quantitative analysis of the published literature, including co-authorship, co-occurrence, citation, and co-citation, to provide objective presentation and predictions in the field.Results: A total of 1595 articles and reviews on MSC-EVs published between 2009 and 2021 were identified. The annual publication outputs increased at an exponential rate, reaching as high as 555 publications in 2021. China contributed the most publications (n = 899, 56.36%) and had the most citations (n = 24,210). The United States had the strongest intensity of cooperation in this field. Shanghai Jiao Tong University had the maximum number of publications (n = 79). In terms of the number of publications and co-citations, the journal of Stem cell research & therapy ranked first. Camussi G was the most productive and most cited author. The top three themes in the research area were cell biology, research experimental medicine, and biochemistry molecular biology. Keyword co-occurrence and co-citation clustering analysis revealed that studies of MSC-EVs covered cellular origin (bone marrow mesenchymal stem cell, adipose-derived mesenchymal stem cell), injurious diseases (spinal cord injury, acute lung injury, ischemia/reperfusion injury, acute kidney injury, traumatic brain injury), tumor (breast cancer, tumor microenvironment), biological processes (drug delivery system, angiogenesis, inflammation, proliferation, differentiation, senescence), and molecular mechanisms (signaling pathway, signal transduction, oxidative stress, VEGF, TGF β).Conclusions: Studies on MSC-EVs have shown a steep growth trend in recent years. Available studies mostly focused on the therapeutic effects and underlying mechanisms of MSC-EVs in aplastic diseases. Multidisciplinary integration is a development trend in this field, and senescence-related topics might be the focus of future research on MSC-EVs.

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“…2 The presence of EVs in biofluids such as urine, 3 blood plasma, 4 serum 5 and saliva, 6 just to name a few, make EVs prospective biomarker candidates that can be readily obtained utilising non-invasive processes for applications in diagnostics. 7 Their broad functional roles in providing a transport network for the exchange of bioactive cargos between cells and tissues of the body also provides a framework for the development of EV therapeutics. EVs are appealing therapeutic candidates due to their inherent biocompatibility, small size and ability to cross complex tissue and cell barriers.…”

Section: Introductionmentioning

confidence: 99%

A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods

et al. 2023

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Extracellular vesicles (EVs) continue to gain interest across the scientific community for diagnostic and therapeutic applications. As EV applications diversify, it is essential that researchers are aware of challenges, in particular the compatibility of EV isolation methods with downstream applications and their clinical translation. We report outcomes of the first cross-comparison study looking to determine parameters (EV source, starting volume, operator experience, application and implementation parameters such as cost and scalability) governing the selection of popular EV isolation methods across disciplines. Our findings highlighted an increased clinical focus, with 36% of respondents applying EVs in therapeutics and diagnostics. Data indicated preferential selection of ultracentrifugation for therapeutic applications, precipitation reagents in clinical settings and size exclusion chromatography for diagnostic applications utilising biofluids. Method selection was influenced by operator experience, with increased method diversity when EV research was not the respondents primary focus. Application and implementation criteria were indicated to be major influencers in method selection, with UC and SEC chosen for their abilities to process large and small volumes, respectively. Overall, we identified parameters influencing method selection across the breadth of EV science, providing a valuable overview of practical considerations for the effective translation of research outcomes.

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Extracellular vesicle-associated proteins as potential biomarkers

Cited by 10 publications

References 172 publications

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

An Overview of Current Research on Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Bibliometric Analysis From 2009 to 2021

A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods

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