Extracellular vesicle-based EGFR genotyping in bronchoalveolar lavage fluid

Epidermal Growth Factor Receptor Mutations Carried in Extracellular Vesicle-Derived Cargo Mirror Disease Status in Metastatic Non-small Cell Lung Cancer

“…Several recent works have demonstrated the feasibility of using EV-RNA to detect EGFR mutations from blood ( Dong et al, 2019 ; Pasini et al, 2021 ) and bronchoalveolar lavage fluid ( Hur et al, 2019 ; Liam et al, 2020 ). Notably, a robust method was developed for detecting mutations in EV-RNA using droplet digital PCR (ddPCR) and was validated with Sanger sequencing with mutations found with over 90% sensitivity.…”