2021
DOI: 10.1002/jev2.12110
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Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV‐CATCHER): A customizable purification assay designed for small‐RNA biomarker identification and evaluation of circulating small‐EVs

Abstract: Circulating nucleic acids, encapsulated within small extracellular vesicles (EVs), provide a remote cellular snapshot of biomarkers derived from diseased tissues, however selective isolation is critical. Current laboratory‐based purification techniques rely on the physical properties of small‐EVs rather than their inherited cellular fingerprints. We established a highly‐selective purification assay, termed EV‐CATCHER, initially designed for high‐throughput analysis of low‐abundance small‐RNA cargos by next‐gen… Show more

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Cited by 31 publications
(56 citation statements)
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“…Several RNA species were detected in large quantity in exosomes (exRNAs), such as snRNAs SNORD33, RNU2-29P; transcripts AL732437.2 and AL365184.1; non-coding RNAs CDKN2B-AS1, and miRNAs miR-122-5p [ 79 ], hsa-miR-146a and hsa-miR126-3p [ 80 ].…”
Section: Extracellular Vesicles As Biomarkersmentioning
confidence: 99%
“…Several RNA species were detected in large quantity in exosomes (exRNAs), such as snRNAs SNORD33, RNU2-29P; transcripts AL732437.2 and AL365184.1; non-coding RNAs CDKN2B-AS1, and miRNAs miR-122-5p [ 79 ], hsa-miR-146a and hsa-miR126-3p [ 80 ].…”
Section: Extracellular Vesicles As Biomarkersmentioning
confidence: 99%
“…Another purification assay was designed for the high-throughput analysis of low abundance miRNAs of EVs and the evaluation of EVs' functional characteristics. The assay, called EV-CATCHER (EV Capture by AnTibody of CHoice and Enzymatic Release), was recently published [29] (Figure 2a), and demonstrated the possibility for use in the isolation of EVs from the serum of hospitalized patients with COVID-19 disease, with two miRNAs, associated with in-flammation and endothelial cell repair inhibition, identified as being downregulated with disease severity (CC8.2).…”
Section: Advances In Ev Isolation and Detectionmentioning
confidence: 99%
“…Capture by AnTibody of CHoice and Enzymatic Release), was recently published [2 (Figure 2a), and demonstrated the possibility for use in the isolation of EVs from the seru of hospitalized patients with COVID-19 disease, with two miRNAs, associated w inflammation and endothelial cell repair inhibition, identified as being downregulat with disease severity (CC8.2). [29] developed for selectiv purifying small-EVs from human biofluids. The assay relies on the binding of a degradable dsDN linker to a DBCO-activated antibody and to a streptavidin-coated platform.…”
Section: Advances In Ev Isolation and Detectionmentioning
confidence: 99%
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