Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy

Kurian, Noble K; Modi, Deepak

doi:10.1007/s10815-018-1343-x

Cited by 113 publications

(109 citation statements)

References 62 publications

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“…Many studies have shown that PA is a specific activator of type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K), which stimulates phosphatidylinositol (4,5) bisphosphate PI(4,5)P 2 production. PA is a positive regulator of PI(4,5)P2, resulting in the accumulation of PA and PI(4,5)P2.…”

Section: Discussionmentioning

confidence: 99%

“…Extracellular vesicles (EVs), such as microvesicles and exosomes, were found to play key roles in embryo implantation, pregnancy, and parturition in recent studies [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, EV contents (such as proteins, nucleic acids and lipids) provide a powerful noninvasive method for predicting the risk of preterm birth. Proteins and nucleic acids in EVs related to preterm birth have been characterized [5,14]. However, the lipids within EVs remain undefined.…”

Section: Introductionmentioning

confidence: 99%

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Lipidomic profiling of plasma extracellular vesicles as an effective means to evaluate the risk of preterm birth

Zhao

Wang

et al. 2020

Preprint

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Preterm birth is the main cause of infant death worldwide and results in a high societal economic burden associated with newborn care. Recent studies have shown that extracellular vesicles play an important role in fetal development during pregnancy. Here, we fully investigated differences in lipids in plasma, microvesicles and exosomes between 27 preterm and 66 full-term pregnant women in the early second trimester (12-24 weeks) using an untargeted lipidomics approach. Independent of other characteristics of samples, we detected 97, 58 and 10 differential features (retention time (RT) and m/z) with identification by multivariate and univariate statistical analyses in plasma, microvesicles and exosomes, respectively. These altered lipids were involved in the formation of the bacterial cell wall and chronic low-level inflammation and oxidative stress. Furthermore, lipids in microvesicles could distinguish patients who experienced preterm labor from controls better than lipids in plasma and exosomes. The candidate lipid biomarkers in microvesicles were also validated by the pseudotargeted lipidomics method. The validation set included 41 preterm and 42 healthy pregnant women. PS (34:0) in microvesicles was able to distinguish preterm birth from healthy pregnancy with higher accuracy. Our study shows that differences in lipids in plasma, microvesicles and exosomes are useful for understanding the underlying mechanisms, early clinical diagnosis and intervention of preterm birth.

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Section: Discussionmentioning

confidence: 99%

“…Extracellular vesicles (EVs), such as microvesicles and exosomes, were found to play key roles in embryo implantation, pregnancy, and parturition in recent studies [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Lipidomic profiling of plasma extracellular vesicles as an effective means to evaluate the risk of preterm birth

Zhao

Wang

et al. 2020

Preprint

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“…For example, it has been shown that endometrial epithelial cells treated with the hormones estrogen and progesterone begin to generate EVs. When these vesicles were isolated and then used to treat cultures of trophoblasts, the cell adhesion protein focal adhesion kinase (FAK) became activated and the adhesive capacity of the trophoblasts was increased, suggesting that EVs generated by maternal tissues may play a role during the implantation process …”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicles and their roles in stem cell biology

2020

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Stem cells use a variety of mechanisms to help maintain their pluripotency and promote self‐renewal, as well as, at the appropriate time, to differentiate into specialized cells. One such mechanism that is attracting significant attention from the stem cell, development, and regenerative medicine research communities involves a form of intercellular communication, specifically, the ability of cells to form and release nontraditional membrane‐enclosed structures, referred to as extracellular vesicles (EVs). There are two major classes of EVs, microvesicles (MVs), which are generated through the outward budding and fission of the plasma membrane, and exosomes, which are formed as multivesicular bodies (MVBs) in the endo‐lysosomal pathway that fuse with the cell surface to release their contents. Although they differ in how they are formed, both MVs and exosomes have been shown to contain a diverse array of bioactive cargo, such as proteins, RNA transcripts, microRNAs, and even DNA, which can be transferred to other cells and promote phenotypic changes. Here, we will describe what is currently known regarding EVs and the roles they play in stem cell biology and different aspects of early development. We will also highlight how the EVs produced by stem cells are being aggressively pursued for clinical applications, including their potential use as therapeutic delivery systems and for their regenerative capabilities.

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“…exosomes of uterine luminal fluid (ULF) are capable of mediating embryo-endometrium cross-talk during implantation [8]. Therefore, in order to improve the rate of successful implantation in pigs, it is necessary to investigate the mechanism by which ULF exosomes regulate the cross-talk.…”

mentioning

confidence: 99%

Small RNA-seq analysis of exosomes from porcine uterine luminal fluid during peri-implantation

Hua

Wang

Lian

et al. 2019

Preprint

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Background The exosomes of uterine luminal fluid (ULF) mediate intrauterine communication between conceptus and uterus in pigs. The small RNAs of ULF exosomes are widely recognized as important factors that influence embryonic implantation in humans, mice, cattle and sheep. Therefore, it can be hypothesized that small RNAs of ULF exosomes are essential for implantation in pigs. Results Cupshaped exosomes (diameters: 40-160 nm) of porcine ULF were isolated and characterized, and then PKH67 stained exosomes were taken up by endometrial epithelial cells and porcine trophectoderm cells. The expression of small RNAs in these exosomes on days 10 (D10), 13 (D13) and 18 (D18) of pregnancy was comprehensively profiled through sequencing. From nine libraries, a total of 152 known microRNAs (miRNAs), 43 novel miRNAs and 145 novel Piwi-interacting RNAs (piRNAs) were identified. Among these small RNAs, ssc-miR-21-5p, ssc-let-7a, ssc-let-7i-5p and ssc-let-7g were differentially and highly expressed during the three stages. Hierarchical cluster analysis and principal component analysis suggested that the expression profiles of small RNAs in D13 group were different from those in D10 and D18 groups. Bioinformatics analysis showed that the miRNAs differentially expressed in the comparisons between D10 and D13, D13 and D18, and D10 and D18 groups were involved in important molecular functions and pathways such as "G-protein coupled receptor activity" and "Insulin signaling pathway". Conclusions Our results reveal that porcine ULF exosomes contain various small RNAs with potentially vital effects on implantation. This research also provides resources for studies of miRNAs and piRNAs in the cross-talk between embryo and endometrium. BackgroundPrevious studies have indicated that the majority of pre-natal losses occur during the periimplantation period in pigs [1,2]. During this period, the porcine blastocyst expands from 0.5-1 mm in diameter at hatching to 2-mm spheres on day 10 of pregnancy (D10) and begins to rapidly elongate into tubular and filamentous shapes on day 11 or 12 [3,4]. The conceptus starts to attach to the surface of maternal endometrium at day 13 of pregnancy (D13) and the attachment is usually completed between days 18 (D18) and 24 of pregnancy [5,6]. The synchronous communication between conceptus and uterus exerts strong influence on embryo implantation [7]. Furthermore, the

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Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy

Cited by 113 publications

References 62 publications

Lipidomic profiling of plasma extracellular vesicles as an effective means to evaluate the risk of preterm birth

Lipidomic profiling of plasma extracellular vesicles as an effective means to evaluate the risk of preterm birth

Extracellular vesicles and their roles in stem cell biology

Small RNA-seq analysis of exosomes from porcine uterine luminal fluid during peri-implantation

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