Extracellular vesicle production by oral bacteria related to dental caries and periodontal disease: role in microbe-host and interspecies interactions

Leiva-Sabadini, Camila; Saavedra, Paula; Inostroza, Carla; Aguayo, Sebastian

doi:10.1080/1040841x.2024.2427656

Critical Reviews in Microbiology

2024

DOI: 10.1080/1040841x.2024.2427656

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Extracellular vesicle production by oral bacteria related to dental caries and periodontal disease: role in microbe-host and interspecies interactions

Camila Leiva-Sabadini,

Paula Saavedra,

Carla Inostroza

et al.

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Biofilm formation on glycated collagen modulatesStreptococcus mutansbacterial extracellular vesicle production and cargo

Leiva-Sabadini,

Berríos,

Saavedra

et al. 2024

Preprint

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Streptococcus mutansis the major microbial etiological agent of dental caries and can adhere to surfaces such as type-I collagen, present in dentin and periodontal tissues. Recent studies have characterized planktonicS. mutansbacterial extracellular vesicles (bEVs) and demonstrated environmental-induced changes due to sugar presence or pH alterations. However, to date there are no studies exploring if surface-derived changes - such as tissue glycation - can modulate bEV production in the context of oral biofilm formation in the elderly. Therefore, the aim of this work was to determine the role of biofilm formation and collagen glycation on the morphology and composition ofS. mutansbEVs. For this, bEVs fromS. mutansbiofilms on native and glycated collagen surfaces were isolated, characterized, and compared to bEVs from planktonic cells. Nanoparticle tracking analysis and microscopy confirmed bEV production and showed that bEVs from biofilms are smaller in size and less abundant than those from planktonic cells. Furthermore, proteome analysis revealed thatS. mutansbiofilm formation on native and glycated collagen led to the enrichment of several key virulence proteins such as Eno, LuxS, Tpx, and ScrB. Also, a shift towards proteins involved in metabolic processes was found in bEVs following biofilm formation on collagen surfaces, whereas glucan metabolism proteins were overexpressed in vesicles from the planktonic state. These results demonstrate that biofilm formation, as well as the glycation of collagen associated with aging and hyperglycemia, can modulate bEV characteristics and cargo and could play a central role inS. mutansvirulence and the development of diseases such as dental caries and periodontal disease.

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Biofilm formation on glycated collagen modulatesStreptococcus mutansbacterial extracellular vesicle production and cargo

Leiva-Sabadini,

Berríos,

Saavedra

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Extracellular vesicle production by oral bacteria related to dental caries and periodontal disease: role in microbe-host and interspecies interactions

Cited by 1 publication

References 172 publications

Biofilm formation on glycated collagen modulatesStreptococcus mutansbacterial extracellular vesicle production and cargo

Biofilm formation on glycated collagen modulatesStreptococcus mutansbacterial extracellular vesicle production and cargo

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