Extracellular Vesicles: Catching the Light in Zebrafish

Verweij, Frederik J.; Hyenne, Vincent; Niel, Guillaume van; Goetz, Jacky G.

doi:10.1016/j.tcb.2019.07.007

Cited by 41 publications

(36 citation statements)

References 63 publications

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“…Our findings complement recent studies that describe the labelling, biodistribution and potential functional roles of exogenous tumour derived and endogenous CD63+ EVs in larval zebrafish (Hyenne et al, 2019;Verweij et al, 2019b). The described techniques and advancements in the use of zebrafish for in vivo EV research pave the way for investigations into multiple homeostatic and pathophysiological states (Verweij et al, 2019a) and the involvement of endogenous vesicles in cellular processes such as tissue regeneration.…”

Section: Discussionsupporting

confidence: 83%

In vivo characterisation of endogenous cardiovascular extracellular vesicles in larval and adult zebrafish

Scott

Ballesteros

Bradshaw

et al. 2019

Preprint

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Ischaemic cardiac injury results in a complex coordinated cellular response that enables effective damage control and recovery. Intercellular communication is integral to the coordination of these repair responses in complex tissue contexts. Extracellular vesicles (EVs) facilitate molecular transport across extracellular space, allowing local and systemic signaling during homeostasis and in disease. Extensive in vitro studies have described the biogenesis, cargos and functional roles of EV populations but the characterisation of endogenously produced EVs in vivo is still in its infancy. Here we use larval and adult transgenic zebrafish to characterize endogenous cardiovascular EVs. Using a membranetethered fluorophore reporter system, cell-type specific EVs can be tracked in vivo by high spatiotemporal resolution light sheet imaging and modified flow cytometry methods allows these EVs to be extracted and assessed ex vivo. Importantly, we show that ischaemic cardiac injury models dynamically alter EV production during repair.

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Section: Discussionsupporting

confidence: 83%

In vivo characterisation of endogenous cardiovascular extracellular vesicles in larval and adult zebrafish

Scott

Ballesteros

Bradshaw

et al. 2019

Preprint

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“…Recently, it was reported that a zebrafish transgenic line that expresses human CD63 fused with pHluorin under the control of tissue-specific promoter/enhancer can be used to visualize the release and uptake of EVs in vivo. A role for EVs in the formation of metastatic niches in vivo could also be determined by tracking EVs shed from melanoma cells in zebrafish embryos, demonstrating that zebrafish is an excellent model for the study of EVs 50 . In the present study, our transplantation assays clearly demonstrated that mCherry + particles observed in trap:GFP + cells are derived from EVs shed from OBs.…”

Section: Discussionmentioning

confidence: 99%

Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale

et al. 2020

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Differentiation of osteoclasts (OCs) from hematopoietic cells requires cellular interaction with osteoblasts (OBs). Due to the difficulty of live-imaging in the bone, however, the cellular and molecular mechanisms underlying intercellular communication involved in OC differentiation are still elusive. Here, we develop a fracture healing model using the scale of trap: GFP; osterix:mCherry transgenic zebrafish to visualize the interaction between OCs and OBs. Transplantation assays followed by flow cytometric analysis reveal that most trap:GFP high OCs in the fractured scale are detected in the osterix:mCherry + fraction because of uptake of OB-derived extracellular vesicles (EVs). In vivo live-imaging shows that immature OCs actively interact with osterix:mCherry + OBs and engulf EVs prior to convergence at the fracture site. In vitro cell culture assays show that OB-derived EVs promote OC differentiation via Rankl signaling. Collectively, these data suggest that EV-mediated intercellular communication with OBs plays an important role in the differentiation of OCs in bone tissue.

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“…Interestingly, all publications exclusively reported the use of mice for exosome biodistribution analysis. Recently, increasing evidence suggests that the use of zebrafish is a promising new approach to study in vivo physiology and pathology of exosomes [101]. Indeed, the transparency and small size of the zebrafish embryo enables live whole-body imaging analysis for better understanding of biodistribution including exosome uptake and fate.…”

Section: Determination Of Exosome Dosementioning

confidence: 99%

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Yi¹,

Lee²,

Kim

et al. 2020

IJMS

146

131

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Exosomes are nano-sized membranous vesicles produced by nearly all types of cells. Since exosome-like vesicles are produced in an evolutionarily conserved manner for information and function transfer from the originating cells to recipient cells, an increasing number of studies have focused on their application as therapeutic agents, drug delivery vehicles, and diagnostic targets. Analysis of the in vivo distribution of exosomes is a prerequisite for the development of exosome-based therapeutics and drug delivery vehicles with accurate prediction of therapeutic dose and potential side effects. Various attempts to evaluate the biodistribution of exosomes obtained from different sources have been reported. In this review, we examined the current trends and the advantages and disadvantages of the methods used to determine the biodistribution of exosomes by molecular imaging. We also reviewed 29 publications to compare the methods employed to isolate, analyze, and label exosomes as well as to determine the biodistribution of labeled exosomes.

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Extracellular Vesicles: Catching the Light in Zebrafish

Cited by 41 publications

References 63 publications

In vivo characterisation of endogenous cardiovascular extracellular vesicles in larval and adult zebrafish

In vivo characterisation of endogenous cardiovascular extracellular vesicles in larval and adult zebrafish

Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

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