Extracellular Vesicles in pregnancy: Their potential role as a liquid biopsy

Buca, Danilo; D’Antonio, F.; Buca, D.; Sebastiano, F. Di; Simeone, Pasquale; Girolamo, Raffaella Di; Bologna, Giuseppina; Vespa, Simone; Catitti, Giulia; Liberati, Marco; Miscia, Sebastianó; Lanuti, Paola

doi:10.1016/j.jri.2022.103734

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Cellular sources included endothelial cells ( n = 22 (FC)) (Abolbaghaei et al., 2021; Alijotas‐Reig et al., 2011, 2012; Bretelle et al., 2003; Buca et al., 2022; Campello et al., 2018; Chen et al., 2012; Ferrari et al., 2022; González‐Quintero et al., 2003, 2004; Hu et al., 2018; Jadli et al., 2019; Lalic‐Cosic et al., 2021; Ling et al., 2014; Lok et al., 2008; Marques et al., 2012; Radu et al., 2015; Salem et al., 2015; VanWijk, Nieuwland et al., 2002a; Zhang et al., 2018), platelets ( n = 23 (FC), (Abolbaghaei et al., 2021; Alasztics et al., 2021; Alijotas‐Reig et al., 2011, 2012; Bretelle et al., 2003; Buca et al., 2022; Campello et al., 2015, 2018; Dragovic et al., 2013; Ferrari et al., 2022; González‐Quintero et al., 2003; Hu et al., 2018; Kovács et al., 2018a, 2018b; Lalic‐Cosic et al., 2021; Lok et al., 2008; Marques et al., 2012; Radu et al., 2015; Salem et al., 2015; Textoris et al., 2013; VanWijk et al., 2002b; Zhang et al., 2018; Chen et al., 2021b), the syncytiotrophoblast ( n = 11 (FC) (Dragovic et al., 2013; Ferrari et al., 2022; Gill et al., 2019; Hu et al., 2018; Kovács et al., 2018a, 2018b; Lok et al., 2008; Marques et al., 2012; Moro et al., 2016; Orozco et al., 2009; VanWijk et al., 2002b; Chen et al., …”

Section: Resultsmentioning

confidence: 99%

“…Cellular sources included endothelial cells (n = 22 (FC)) (Abolbaghaei et al, 2021;Alijotas-Reig et al, 2011Bretelle et al, 2003;Buca et al, 2022;Campello et al, 2018;Chen et al, 2012;Ferrari et al, 2022;González-Quintero et al, 2003, 2004Hu et al, 2018;Jadli et al, 2019;Lalic-Cosic et al, 2021;Ling et al, 2014;Lok et al, 2008;Marques et al, 2012;Radu et al, 2015;Salem et al, 2015;VanWijk, Nieuwland et al, 2002a;Zhang et al, 2018), platelets (n = 23 (FC), (Abolbaghaei et al, 2021;Alasztics et al, 2021;Alijotas-Reig et al, 2011Bretelle et al, 2003;Buca et al, 2022;Campello et al, 2015Campello et al, , 2018Dragovic et al, 2013;Ferrari et al, 2022;González-Quintero et al, 2003;Hu et al, 2018;Kovács et al, 2018aKovács et al, , 2018bLalic-Cosic et al, 2021;Lok et al, 2008;Marques et al, 2012;Radu et al, 2015;Salem et al, 2015;Textoris et al, 2013;VanWijk et al, 2002b;Zhang et al, 2018;Chen et al, 2021b), the syncytiotrophoblast (n = 11 (FC)…”

Section:  Cellular Source Of Circulating Evs In Pregnancymentioning

confidence: 99%

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Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results

Barnes,

Pantazi,

Holder

2023

J of Extracellular Vesicle

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Extracellular vesicles (EVs) play a crucial role in pregnancy, revealed by the presence of placental‐derived EVs in maternal blood, their in vitro functionality, and their altered cargo in pregnancy pathologies. These EVs are thought to be involved in the development of pregnancy pathologies, such as pre‐eclampsia, pre‐term birth, and fetal growth restriction, and have been suggested as a source of biomarkers for gestational diseases. However, to accurately interpret their function and biomarker potential, it is necessary to critically evaluate the EV isolation and characterization methodologies used in pregnant cohorts. In this systematic scoping review, we collated the results from 152 studies that have investigated EVs in the blood of pregnant women, and provide a detailed analysis of the EV isolation and characterization methodologies used. Our findings indicate an overall increase in EV concentrations in pregnant compared to non‐pregnant individuals, an increased EV count as gestation progresses, and an increased EV count in some pregnancy pathologies. We highlight the need for improved standardization of methodology, greater focus on gestational changes in EV concentrations, and further investigations into the functionality of EVs. Our review suggests that EVs hold great promise as diagnostic and translational tools for gestational diseases. However, to fully realize their potential, it is crucial to improve the standardization and reliability of EV isolation and characterization methodologies, and to gain a better understanding of their functional roles in pregnancy pathologies.

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Section: Resultsmentioning

confidence: 99%

Section:  Cellular Source Of Circulating Evs In Pregnancymentioning

confidence: 99%

Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results

Barnes,

Pantazi,

Holder

2023

J of Extracellular Vesicle

View full text Add to dashboard Cite

show abstract

“…For example, platelet-, endothelial-, and leukocyte-derived EVs can be identified and counted from whole peripheral blood samples [34,37]. Furthermore, circulating EVs, reflecting the physiological and pathophysiological condition of the body, have been identified as reliable biomarkers for liquid biopsy purposes [42][43][44]. Peripheral EV concentrations, as well as their cargo composition, are characterized by a profound inter-subject variability, indicating that their release is a highly dynamic process [45].…”

Section: Extracellular Vesicle-mediated Intercellular Crosstalkmentioning

confidence: 99%

Extracellular Vesicles as Players in the Anti-Inflammatory Inter-Cellular Crosstalk Induced by Exercise Training

Catitti

Bellis

Vespa

et al. 2022

IJMS

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Extracellular Vesicles (EVs) are circulating particles surrounded by a plasma membrane carrying a cargo consisting of proteins, lipids, RNAs, and DNA fragments, stemming from the cells from which they originated. EV factors (i.e., miRNAs) play relevant roles in intercellular crosstalk, both locally and systemically. As EVs increasingly gained attention as potential carriers for targeted genes, the study of EV effects on the host immune response became more relevant. It has been demonstrated that EVs regulate the host immune response, executing both pro- and anti-inflammatory functions. It is also known that physical exercise triggers anti-inflammatory effects. This review underlines the role of circulating EVs as players in the anti-inflammatory events associated with the regulation of the host’s immune response to physical exercise.

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“…In any case, EVs have been identified in many body fluids, such as peripheral blood, cerebrospinal fluid, and tears [ 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. EVs also express surface antigens that allow them to reach target cells [ 26 ]. Once attached the related recipient cell, EVs activate specific signaling responses interacting with their ligands; EVs can be also internalized by endocytosis and/or phagocytosis or can fuse with the membrane of the target cell, therefore delivering their cargo into the target cell cytosol, modifying the recipient cell biology [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Vesicles in Regenerative Processes Associated with Muscle Injury Recovery of Professional Athletes Undergoing Sub Maximal Strength Rehabilitation

Catitti

Cufaro

Bellis

et al. 2022

IJMS

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Platelet-rich plasma (PRP) has great potential in regenerative medicine. In addition to the well-known regenerative potential of secreted growth factors, extracellular vesicles (EVs) are emerging as potential key players in the regulation of tissue repair. However, little is known about their therapeutic potential as regenerative agents. In this study, we have identified and subtyped circulating EVs (platelet-, endothelial-, and leukocyte-derived EVs) in the peripheral blood of athletes recovering from recent muscular injuries and undergoing a submaximal strength rehabilitation program. We found a significant increase in circulating platelet-derived EVs at the end of the rehabilitation program. Moreover, EVs from PRP samples were isolated by fluorescence-activated cell sorting and analyzed by label-free proteomics. The proteomic analysis of PRP-EVs revealed that 32% of the identified proteins were associated to “defense and immunity”, and altogether these proteins were involved in vesicle-mediated transport (GO: 0016192; FDR = 3.132 × 10−19), as well as in wound healing (GO: 0042060; FDR = 4.252 × 10−13) and in the events regulating such a process (GO: 0061041; FDR = 2.812 × 10−12). Altogether, these data suggest that platelet-derived EVs may significantly contribute to the regeneration potential of PRP preparations.

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Extracellular Vesicles in pregnancy: Their potential role as a liquid biopsy

Cited by 6 publications

References 25 publications

Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results

Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results

Extracellular Vesicles as Players in the Anti-Inflammatory Inter-Cellular Crosstalk Induced by Exercise Training

Extracellular Vesicles in Regenerative Processes Associated with Muscle Injury Recovery of Professional Athletes Undergoing Sub Maximal Strength Rehabilitation

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