Extracellular vesicles in the context of Mycobacterium tuberculosis infection

Palacios, Ainhoa; Gupta, Shamba; Rodríguez, G. Marcela; Prados-Rosales, Rafael

doi:10.1016/j.molimm.2021.02.010

Cited by 29 publications

(38 citation statements)

References 48 publications

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“…The recent discovery of several mechanisms by which the pathogen releases extracellular vesicles (EVs) could also provide an explanation for our observation. While none of the Mtb proteins detected here have been reported in Mtb vesicles, EV composition is known to vary; thereby, more work is needed to highlight the compositional heterogeneity of Mtb vesicles. Thus, these findings highlight the need for unbiased analysis of biofluids to gain insights into TB biology.…”

Section: Discussionmentioning

confidence: 88%

Toward Comprehensive Plasma Proteomics by Orthogonal Protease Digestion

et al. 2021

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Rapid and consistent protein identification across large clinical cohorts is an important goal for clinical proteomics. With the development of data-independent technologies (DIA/SWATH-MS), it is now possible to analyze hundreds of samples with great reproducibility and quantitative accuracy. However, this technology benefits from empirically derived spectral libraries that define the detectable set of peptides and proteins. Here, we apply a simple and accessible tip-based workflow for the generation of spectral libraries to provide a comprehensive overview on the plasma proteome in individuals with and without active tuberculosis (TB). To boost protein coverage, we utilized nonconventional proteases such as GluC and AspN together with the gold standard trypsin, identifying more than 30,000 peptides mapping to 3309 proteins. Application of this library to quantify plasma proteome differences in TB infection recovered more than 400 proteins in 50 min of MS acquisition, including diagnostic Mycobacterium tuberculosis (Mtb) proteins that have previously been detectable primarily by antibody-based assays and intracellular proteins not previously described to be in plasma.

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Section: Discussionmentioning

confidence: 88%

Toward Comprehensive Plasma Proteomics by Orthogonal Protease Digestion

et al. 2021

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“…Moreover, Toll-like receptors (TLRs) and NOD-like receptors (NLRs) are critical pattern recognition receptors (PRRs), involved in direct host-bacterial interactions through bacterial EVs [ 45 ]. The involvement of TLR2 in the internalization process of EVs derived from Moraxella catarrhalis and Mycobacterium in epithelial cells has been established [ 46 , 47 ]. The interaction of Bifidobacterium and Lactobacillus -derived EVs with dendritic cells enhances TLR2/1 and TLR4 responses [ 48 ].…”

Section: The Importance Of Bacterial Evs In Host-bacterial Dialoguesmentioning

confidence: 99%

Commensal and Pathogenic Bacterial-Derived Extracellular Vesicles in Host-Bacterial and Interbacterial Dialogues: Two Sides of the Same Coin

Tarashi

Zamani

Omrani

et al. 2022

Journal of Immunology Research

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Extracellular vesicles (EVs) cause effective changes in various domains of life. These bioactive structures are essential to the bidirectional organ communication. Recently, increasing research attention has been paid to EVs derived from commensal and pathogenic bacteria in their potential role to affect human disease risk for cancers and a variety of metabolic, gastrointestinal, psychiatric, and mental disorders. The present review presents an overview of both the protective and harmful roles of commensal and pathogenic bacteria-derived EVs in host-bacterial and interbacterial interactions. Bacterial EVs could impact upon human health by regulating microbiota–host crosstalk intestinal homeostasis, even in distal organs. The importance of vesicles derived from bacteria has been also evaluated regarding epigenetic modifications and applications. Generally, the evaluation of bacterial EVs is important towards finding efficient strategies for the prevention and treatment of various human diseases and maintaining metabolic homeostasis.

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“…Interestingly, many of them, particularly the most abundant, were previously described to be released in bacterial-and/or host-derived extracellular vesicles [32][33][34][35] (Supplementary Table 8).…”

Section: Ebcs From Tb Patients Contain M Tuberculosis Proteins Found ...mentioning

confidence: 99%

A Mycobacterium tuberculosis fingerprint in human breath allows tuberculosis diagnosis

Mosquera-Restrepo¹,

Zuberogoïtia

Gouxette

et al. 2022

Preprint

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An estimated one third of tuberculosis (TB) cases go undiagnosed or unreported. Sputum samples, widely used for TB diagnosis, are inefficient at detecting infection in children and paucibacillary (smear negative) patients. Indeed, developing point-of-care biomarker-based diagnostics that are not sputum-based is a major priority for the WHO. Here, we tested exhaled breath condensate (EBC) for Mycobacterium tuberculosis (Mtb) molecules and assessed whether this approach allows pulmonary TB diagnosis. Mtb-specific lipids, lipoarabinomannan lipoglycan, and proteins present in EBCs unambiguously differentiate TB patients from controls. We used EBCs to track the longitudinal effects of antibiotic treatment in Mtb-infected children. In addition, Mtb lipoarabinomannan and lipid structure in EBC revealed specific metabolic and biochemical states of Mtb in the human lung. Our data collectively indicate that EBC analysis can unequivocally diagnose TB across all patient populations and monitor treatment efficacy. This affordable, rapid and non-invasive approach seems superior to sputum assays and can potentially easily be implemented at point-of-care.

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Extracellular vesicles in the context of Mycobacterium tuberculosis infection

Cited by 29 publications

References 48 publications

Toward Comprehensive Plasma Proteomics by Orthogonal Protease Digestion

Toward Comprehensive Plasma Proteomics by Orthogonal Protease Digestion

Commensal and Pathogenic Bacterial-Derived Extracellular Vesicles in Host-Bacterial and Interbacterial Dialogues: Two Sides of the Same Coin

A Mycobacterium tuberculosis fingerprint in human breath allows tuberculosis diagnosis

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