Extracellular Vesicles’ Role in Angiogenesis and Altering Angiogenic Signaling

Ateeq, Maryam; Broadwin, Mark; Sellke, Frank W.; Abid, M. Ruhul

doi:10.3390/medsci12010004

Cited by 5 publications

(2 citation statements)

References 121 publications

(192 reference statements)

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“…The exact mechanism is still unclear. It seems that B cells generate IL-10 and TGF-β as proinflammatory mediators and TNF-α, IL-6, and matrix metalloproteinases as anti-inflammatory factors [55].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Immunohistochemical Biomarkers in Diabetic Wistar Rats with Periodontal Disease

Scrobota,

Tig,

Marcu

et al. 2024

JPM

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Background: The association of periodontal disease and diabetes is a subject of intense research in terms of etiopathology and treatment options. This research aimed to evaluate the modulation of the local inflammatory status by two natural extracts, curcumin (Cu) and rutin (R), in an experimentally induced diabetes and periodontal disease in Wistar rats. Methods: Fifty Wistar albino rats were randomly assigned to five groups: Control (C), Diabetes-associated Periodontal Disease (DP), Diabetes-associated Periodontal Disease treated with Curcumin (DPCu), Diabetes-associated Periodontal Disease treated with Rutin (DPR), and Diabetes-associated Periodontal Disease treated with both Curcumin and Rutin (DPCuR). Gingival samples were collected from all rats, and immunohistochemical markers CD3, CD20, and CD34 were evaluated to assess the local inflammatory infiltrate. Descriptive statistics were applied (SPSS24 Software, Armonk, NY, USA). Results: Rutin, alone or combined with Curcumin, reduced CD3-positive cell levels. Curcumin demonstrated superior efficacy in reducing CD20-positive cells. The combination of Curcumin and Rutin had the most important impact on both markers. Curcumin notably increased immature CD34-positive cell levels. Conclusions: Curcumin and Rutin, either alone or together, hold potential for reducing local inflammation in diabetes-induced periodontal disease in Wistar rats.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Immunohistochemical Biomarkers in Diabetic Wistar Rats with Periodontal Disease

Scrobota,

Tig,

Marcu

et al. 2024

JPM

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“…In microvasculature, the angiogenic response involves changes in cellular adhesive interactions between adjacent ECs, pericytes, and the surrounding ECM. Active neovascularization includes the reorganization of ECs' cytoskeleton, expression of cell surface adhesion molecules like integrins and selectins, secretion of proteolytic enzymes, and remodeling of the adjacent ECM, culminating in the formation of capillary buds [34].…”

Section: Possible Molecular Mechanisms Of Tumor Angiogenesis: Angioge...mentioning

confidence: 99%

Decoding Tumor Angiogenesis for Therapeutic Advancements: Mechanistic Insights

Kaur,

Roy

2024

Biomedicines

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Tumor angiogenesis, the formation of new blood vessels within the tumor microenvironment, is considered a hallmark of cancer progression and represents a crucial target for therapeutic intervention. The tumor microenvironment is characterized by a complex interplay between proangiogenic and antiangiogenic factors, regulating the vascularization necessary for tumor growth and metastasis. The study of angiogenesis involves a spectrum of techniques, spanning from biomarker assessment to advanced imaging modalities. This comprehensive review aims to provide insights into the molecular intricacies, regulatory dynamics, and clinical implications of tumor angiogenesis. By delving into these aspects, we gain a deeper understanding of the processes driving vascularization in tumors, paving the way for the development of novel and effective antiangiogenic therapies in the fight against cancer.

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Endothelial extracellular vesicles enhance vascular self-assembly in engineered human cardiac tissues

Wagner,

Lu,

Landau

et al. 2024

Biofabrication

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The fabrication of complex and stable vasculature in engineered cardiac tissues represents a significant hurdle towards building physiologically relevant models of the heart. Here, we implemented a 3D model of cardiac vasculogenesis, incorporating endothelial cells (EC), stromal cells, and human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) in a fibrin hydrogel. The presence of CMs disrupted vessel formation in 3D tissues, resulting in the upregulation of endothelial activation markers and altered extracellular vesicle (EV) signaling in engineered tissues as determined by the proteomic analysis of culture supernatant. miRNA sequencing of CM- and EC-secreted EVs highlighted key EV-miRNAs that were postulated to play differing roles in cardiac vasculogenesis, including the let-7 family and miR-126-3p in EC-EVs. In the absence of CMs, the supplementation of CM-EVs to EC monolayers attenuated EC migration and proliferation and resulted in shorter and more discontinuous self-assembling vessels when applied to 3D vascular tissues. In contrast, supplementation of EC-EVs to the tissue culture media of 3D vascularized cardiac tissues mitigated some of the deleterious effects of CMs on vascular self-assembly, enhancing the average length and continuity of vessel tubes that formed in the presence of CMs. Direct transfection validated the effects of the key EC-EV miRNAs let-7b-5p and miR-126-3p in improving the maintenance of continuous vascular networks. EC-EV supplementation to biofabricated cardiac tissues and microfluidic devices resulted in tissue vascularization, illustrating the use of this approach in the engineering of enhanced, perfusable, microfluidic models of the myocardium.

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Extracellular Vesicles’ Role in Angiogenesis and Altering Angiogenic Signaling

Cited by 5 publications

References 121 publications

Evaluation of Immunohistochemical Biomarkers in Diabetic Wistar Rats with Periodontal Disease

Evaluation of Immunohistochemical Biomarkers in Diabetic Wistar Rats with Periodontal Disease

Decoding Tumor Angiogenesis for Therapeutic Advancements: Mechanistic Insights

Endothelial extracellular vesicles enhance vascular self-assembly in engineered human cardiac tissues

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