Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells

Gebremedhn, Samuel; Gad, Ahmed; Aglan, Hoda Samir; Laurinčík, Jozef; Procházka, Radek; Salilew‐Wondim, Dessie; Hoelker, M.; Schellander, K.; Tesfaye, Dawit

doi:10.1038/s41598-020-72706-z

Cited by 34 publications

(29 citation statements)

References 74 publications

(96 reference statements)

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“…Currently, it is still unclear whether bystander effects are beneficial, natural defence mechanisms triggered by stressors and mediated by compounds originating from stressed cells or, rather, if they induce deleterious modifications in recipient cells. It is possible that exposure to EVs from stressed cells leads to a higher level of stress in recipient bystander cells, but it also induces more resistance and robustness against subsequent stress, making the bystander effect an adaptive response [ 61 , 124 , 125 , 126 ]. Understanding the molecular mechanisms and signalling pathways of bystander effects and the exact role of EVs in this process is important for a better understanding and estimation of cancer risks and other health consequences associated with ionising radiation exposure.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Gene Expression Modifications Mediated by Extracellular Vesicles: An In Vivo Study of the Radiation-Induced Bystander Effect

Hargitai¹,

Kis²,

Persa³

et al. 2021

Antioxidants

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Radiation-induced bystander effect is a biological response in nonirradiated cells receiving signals from cells exposed to ionising radiation. The aim of this in vivo study was to analyse whether extracellular vesicles (EVs) originating from irradiated mice could induce modifications in the redox status and expression of radiation-response genes in bystander mice. C57BL/6 mice were whole-body irradiated with 0.1-Gy and 2-Gy X-rays, and EVs originating from mice irradiated with the same doses were injected into naïve, bystander mice. Lipid peroxidation in the spleen and plasma reactive oxygen metabolite (ROM) levels increased 24 h after irradiation with 2 Gy. The expression of antioxidant enzyme genes and inducible nitric oxide synthase 2 (iNOS2) decreased, while cell cycle arrest-, senescence- and apoptosis-related genes were upregulated after irradiation with 2 Gy. In bystander mice, no significant alterations were observed in lipid peroxidation or in the expression of genes connected to cell cycle arrest, senescence and apoptosis. However, there was a systemic increase in the circulating ROM level after an intravenous EV injection, and EVs originating from 2-Gy-irradiated mice caused a reduced expression of antioxidant enzyme genes and iNOS2 in bystander mice. In conclusion, we showed that ionising radiation-induced alterations in the cellular antioxidant system can be transmitted in vivo in a bystander manner through EVs originating from directly irradiated animals.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Gene Expression Modifications Mediated by Extracellular Vesicles: An In Vivo Study of the Radiation-Induced Bystander Effect

Hargitai¹,

Kis²,

Persa³

et al. 2021

Antioxidants

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“…Similarly, heat stress-related EVs induced adaptive responses in cultured bovine granulosa cells exposed to heat shock. This observation was evidenced by improved granulosa cell viability and decreased ROS accumulation and was mediated through transfer of HSP90, HSP70, NRF2, antioxidants, and several other microRNAs [121,122].…”

Section: Heat-shockmentioning

confidence: 92%

Role of Extracellular Vesicles in Compromising Cellular Resilience to Environmental Stressors

Alharbi

Lee

Abdelazim

et al. 2021

BioMed Research International

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Extracellular vesicles (EVs), like exosomes, are nanosized membrane-enveloped vesicles containing different bioactive cargo, such as proteins, lipids, mRNA, miRNA, and other small regulatory RNAs. Cell-derived EVs, including EVs originating from stem cells, may capture components from damaged cells or cells impacted by therapeutic treatments. Interestingly, EVs derived from stem cells can be preconditioned to produce and secrete EVs with different therapeutic properties, particularly with respect to heat-shock proteins and other molecular cargo contents. This behavior is consistent with stem cells that also respond differently to various microenvironments. Heat-shock proteins play roles in cellular protection and mediate cellular resistance to radiotherapy, chemotherapy, and heat shock. This review highlights the possible roles EVs play in mediating cellular plasticity and survival when exposed to different physical and chemical stressors, with a special focus on the respiratory distress due to the air pollution.

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“…Real-time PCR reaction was conducted using Maxima SYBR Green/ROX qPCR Master Mix (Thermofisher Scientific, Vilnius, Lithuania) in a StepOnePlus instrument (Applied Biosystems, Foster City, CA, USA) in a reaction mixture (20 µL) consisting of 10 µL of SYBR green with ROX, 7.8 µL of nuclease-free water, 0.2 µL of specific-reverse primer, 0.2 µL of specific-forward primer, and 2 µL of the cDNA sample. The reaction conditions were as follows: 50 • C for 2 min, initial denaturation at 95 • C for 10 min, then 40 PCR cycles consisting of 15 s for denaturation at 95 • C, 1 min for annealing at 60 • C, and 30 s for extension at 72 • C; while 5 min for final extension at 72 • C. Data of gene expression were analyzed using the delta-delta Ct method and reported as relative transcript abundance after normalization to the housekeeping gene Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) according to Khan et al 2020 [14] and Gebremedhn et al [15].…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition

Faheem

Ghanem

Gad

et al. 2021

Animals

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The steroidogenesis capacity and adaptive response of follicular granulosa cells (GCs) to heat stress were assessed together with the underlying regulating molecular mechanisms in Egyptian buffalo. In vitro cultured GCs were exposed to heat stress treatments at 39.5, 40.5, or 41.5 °C for the final 24 h of the culture period (7 days), while the control group was kept under normal conditions (37 °C). Comparable viability was observed between the control and heat-treated GCs at 39.5 and 40.5 °C. A higher release of E2, P4 and IGF-1 was observed in the 40.5 °C group compared with the 39.5 or 41.5 °C groups. The total antioxidant capacity was higher in response to heat stress at 39.5 °C. At 40.5 °C, a significant upregulation pattern was found in the expression of the stress resistance transcripts (SOD2 and NFE2L2) and of CPT2. The relative abundance of ATP5F1A was significantly downregulated for all heat-treated groups compared to the control, while TNFα was downregulated in GCs at 39.5 °C. Expression analyses of stress-related miRNAs (miR-1246, miR-181a and miR-27b) exhibited a significant downregulation in the 40.5 °C group compared to the control, whereas miR-708 was upregulated in the 39.5 and 40.5 °C groups. In conclusion, buffalo GCs exhibited different adaptive responses, to the different heat stress conditions. The integration mechanism between the molecular and secretory actions of the GCs cultured at 40.5 °C might provide possible insights into the biological mechanism through which buffalo GCs react to heat stress.

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Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells

Cited by 34 publications

References 74 publications

Oxidative Stress and Gene Expression Modifications Mediated by Extracellular Vesicles: An In Vivo Study of the Radiation-Induced Bystander Effect

Oxidative Stress and Gene Expression Modifications Mediated by Extracellular Vesicles: An In Vivo Study of the Radiation-Induced Bystander Effect

Role of Extracellular Vesicles in Compromising Cellular Resilience to Environmental Stressors

Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress under In Vitro Condition

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