Extracellular vesicles‐transmitted long non‐coding RNA MTUS2‐5 promotes proliferation and vascularization of human vascular endothelial cells in patients with Budd–Chiari syndrome

Abstract

The high rates of misdiagnosis and untreated mortality with regard to Budd–Chiari syndrome (BCS) indicated the need to screen effective biomarkers. The aim of this study was to explore the function of extracellular vesicles (EVs) in patients with BCS as well as associated mechanisms. First, differentially expressed long non‐coding RNAs (lncRNAs) from EVs separated from serum between BCS and healthy controls were screened using microarray analysis. Second, the proliferation, migration and tube formation of human vascular endothelial cells (HUVECs) were detected after EVs treatment, along with vascular endothelial growth factor (VEGF) levels and inflammatory factors from the cell supernatant. Last, the overexpressed lncRNA was transfected into the cells to further explore the mechanisms involved. Extracellular vesicles of BCS patients have significantly higher levels of lncRNA MTUS2‐5 than healthy controls. Apparently, treatment with EVs from BCS or the ones transfected with plasmids that overexpress lncRNA MTUS2‐5 enhances proliferation, migration and angiogenesis capacity. The results were considerably better than those obtained from treatment with EVs from healthy controls or transfection with the normal control plasmid, which also elevated the level of VEGF and inflammatory factors. Furthermore, FOS and PTGS2 were potentially regulated by the lncRNA MTUS2‐5 transmitted by EVs. The lncRNA MTUS2‐5 in EVs plays an important role in angiogenesis in the Budd–Chiari syndrome.

Vascular dysfunction is at the onset of oxaliplatin-induced peripheral neuropathy symptoms in mice

“…Notably, among the upregulated DEGs, the corticosterone biosynthetic gene Hsd11b1 (Fig 5A) was also found upregulated in the bone marrow of rats treated with a combination of topotecan and oxaliplatin (Davis et al, 2023). Furthermore, the upregulation of the microtubule-associated tumour suppressor gene Mtus2 , shown to limit the metastatic activity of cancer cells (Kuo et al, 2017) and to express a long non-coding RNA Mtus2-5 playing a key role in the angiogenesis of patients with a multifactorial thrombosis condition (Zhang et al, 2023), might be of interest as a downstream target of oxaliplatin IV treatment. Likewise, the upregulated Rasl10b gene encoding a small GTPase with tumour suppressor potential (Zou et al, 2006), which was shown to regulate blood flow in the atrium (Rybkin et al, 2007), could also participate in the antitumoral response downstream of oxaliplatin.…”

lncRNA Profiling of Exosomes and Its Communication Role in Regulating Silica-Stimulated Macrophage Apoptosis and Fibroblast Activation

“…A certain degree of inconsistency between RT-PCR and RNA-seq (around 30-40%) is normal and reasonable, and the two cannot correspond to each other. Studies have shown that the correlation between RNA-seq and RT-PCR is around 0.8, 15.1-19.4% of RNA-seq results do not correspond to RT-PCR, and 1.6-2.8% of nonconcordant sequences are the exact opposite of RT-PCR results [27,[37][38][39].…”