Extracorporeal Shock Wave Therapy Induces Alveolar Bone Regeneration

Sathishkumar, S.; Meka, A.; Dawson, Dolphus R.; House, Nancy S.; Schaden, Wolfgang; Novak, M. John; Ebersole, J. L.; Kesavalu, Lakshmyya

doi:10.1177/154405910808700703

Cited by 50 publications

(57 citation statements)

References 29 publications

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“…In addition, adequate measures were taken to minimize pain or discomfort to mice during oral infection and sampling. Mice were administered sulfamethoxazole (0.87 mg/ml) and trimethoprim (0.17 mg/ml) daily for 10 days in the drinking water (22), and the oral cavity was swabbed with 0.12% chlorhexidine gluconate (Peridex 3 M; ESPE Dental Products, St. Paul, MN) mouth rinse (19,41) to inhibit the endogenous organisms and to promote subsequent colonization with P. gingivalis, T. denticola, and T. forsythia (19). After a 3-day antibiotic washout period, mice were randomized into two groups, followed by oral infection.…”

Section: Methodsmentioning

confidence: 99%

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease

et al. 2011

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Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia are periodontal pathogens associated with the etiology of adult periodontitis as polymicrobial infections. Recent studies demonstrated that oral infection with P. gingivalis induces both periodontal disease and atherosclerosis in hyperlipidemic and proatherogenic ApoE ؊/؊ mice. In this study, we explored the expression of microRNAs (miRNAs) in maxillas (periodontium) and spleens isolated from ApoE ؊/؊ mice infected with P. gingivalis, T. denticola, and T. forsythia as a polymicrobial infection. miRNA expression levels, including miRNA miR-146a, and associated mRNA expression levels of the inflammatory cytokines tumor necrosis factor alpha (TNF-␣) and interleukin-1␤ (IL-1␤) were measured in the maxillas and spleens from mice infected with periodontal pathogens and compared to those in the maxillas and spleens from sham-infected controls. Furthermore, in response to these periodontal pathogens (as mono-and polymicrobial heat-killed and live bacteria), human THP-1 monocytes demonstrated similar miRNA expression patterns, including that of miR-146a, in vitro. Strikingly, miR-146a had a negative correlation with TNF-␣ secretion in vitro, reducing levels of the adaptor kinases IL-1 receptorassociated kinase 1 (IRAK-1) and TNF receptor-associated factor 6 (TRAF6). Thus, our studies revealed a persistent association of miR-146a expression with these periodontal pathogens, suggesting that miR-146a may directly or indirectly modulate or alter the chronic periodontal pathology induced by these microorganisms.

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Section: Methodsmentioning

confidence: 99%

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease

et al. 2011

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“…Several in vitro studies have shown that shock waves have a bactericidal effect on Streptococcus aureus, Streptococcus epidermidis, Pseudomonas aeruginosa, and a MRSA 27065 strain, with decreases in viable numbers of 3 orders of magnitude for certain species [11]. Previous reports have shown that shock wave therapy can kill some oral bacteria and exhibit a beneficial effect during the regeneration process of periodontal disease in a rat model [12,13].…”

Section: Introductionmentioning

confidence: 99%

Potential of shock waves to remove calculus and biofilm

et al. 2010

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Effective calculus and biofilm removal is essential to treat periodontitis. Sonic and ultrasonic technologies are used in several scaler applications. This was the first feasibility study to assess the potential of a shock wave device to remove calculus and biofilms and to kill bacteria. Ten extracted teeth with visible subgingival calculus were treated with either shock waves for 1 min at an energy output of 0.4 mJ/mm(2) at 3 Hz or a magnetostrictive ultrasonic scaler at medium power setting for 1 min, which served as a control. Calculus was determined before and after treatment planimetrically using a custom-made software using a grey scale threshold. In a second experiment, multispecies biofilms were formed on saliva-preconditioned bovine enamel discs during 64.5 h. They were subsequently treated with shock waves or the ultrasonic scaler (N = 6/group) using identical settings. Biofilm detachment and bactericidal effects were then assessed. Limited efficiency of the shock wave therapy in terms of calculus removal was observed: only 5% of the calculus was removed as compared to 100% when ultrasound was used (P

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“…Sham-infected control rats received the vehicle (sterile CMC) only. Oral microbial samples obtained from isoflurane-anesthetized rats were collected at pre-and postinfections, as described previously (32,53). In order to monitor the colonization/infection with minimal disruption of the biofilms, a total of 2 postinfection microbial samples (following the fourth and sixth infections) were collected from all infected rats.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, adequate measures were taken to minimize pain or discomfort of rats during oral infection and sampling. Rats were administered kanamycin (20 mg/rat) and ampicillin (20 mg/rat) daily for 4 days in the drinking water (32,33,53), and the oral cavity was swabbed with 0.12% chlorhexidine gluconate (Peridex; 3M Espe Dental Products, St. Paul, MN) mouth rinse (32,53) to inhibit endogenous organisms and to promote subsequent colonization of P. gingivalis. After a 3-day antibiotic washout period, rats were randomized into three groups (n ϭ 9).…”

Section: Methodsmentioning

confidence: 99%

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Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

Bainbridge¹,

Verma²,

Eastman³

et al. 2010

Infect Immun

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Porphyromonas gingivalis secretes a serine phosphatase enzyme, SerB, upon contact with gingival epithelial cells in vitro. The SerB protein plays a critical role in internalization and survival of the organism in epithelial cells. SerB is also responsible for the inhibition of interleukin-8 (IL-8) secretion from gingival epithelial cells infected with P. gingivalis. This study examined the ability of a P. gingivalis SerB mutant to colonize the oral cavity and induce gingival inflammation, immune responses, and alveolar bone resorption in a rat model of periodontal disease. Both P. gingivalis ATCC 33277 and an isogenic ⌬SerB mutant colonized the oral cavities of rats during the 12-week experimental period. Both of the strains induced significant (P < 0.05) systemic levels of immunoglobulin G (IgG) and isotypes IgG1, IgG2a, and IgG2b, indicating the involvement of both T helper type 1 (Th1) and Th2 responses to infection. Both strains induced significantly (P < 0.05) higher levels of alveolar bone resorption in infected rats than in sham-infected control rats. However, horizontal and interproximal alveolar bone resorption induced by the SerB mutant was significantly (P < 0.05) lower than that induced by the parental strain. Rats infected with the ⌬SerB mutant exhibited significantly higher levels of apical migration of the junctional epithelium (P < 0.01) and polymorphonuclear neutrophil (PMN) recruitment (P < 0.001) into the gingival tissues than rats infected with the wild type. In conclusion, in a rat model of periodontal disease, the SerB phosphatase of P. gingivalis is required for maximal alveolar bone resorption, and in the absence of SerB, more PMNs are recruited into the gingival tissues.

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Extracorporeal Shock Wave Therapy Induces Alveolar Bone Regeneration

Abstract: ESWT, extracorporeal shock wave therapy; PCR, polymerase chain-reaction.

Cited by 50 publications

References 29 publications

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease

Potential of shock waves to remove calculus and biofilm

Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

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Extracorporeal Shock Wave Therapy Induces Alveolar Bone Regeneration

Abstract: ESWT, extracorporeal shock wave therapy; PCR, polymerase chain-reaction.

Cited by 50 publications

References 29 publications

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE −/− Mice during Experimental Periodontal Disease

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE −/− Mice during Experimental Periodontal Disease

Potential of shock waves to remove calculus and biofilm

Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

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Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease

Polymicrobial Infection with Periodontal Pathogens Specifically Enhances MicroRNA miR-146a in ApoE ^−/− Mice during Experimental Periodontal Disease