Extract of Achillea fragrantissima Downregulates ROS Production and Protects Astrocytes from Oxidative-Stress-Induced Cell Death

Elmann, Anat; Telerman, Alona; Mordechay, Sharon; Erlank, Hilla; Rindner, Miriam; Ofir, Rivka; Beit‐Yannai, Elie

doi:10.5772/32398

Cited by 7 publications

(4 citation statements)

References 71 publications

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“…Af is a desert plant that belongs to the Asteraceae family and has been used in traditional medicine for the treatment of respiratory diseases and gastrointestinal disturbances [12][13][14][15]. In previous studies, we have demonstrated that Af extract has anti-inflammatory and antioxidant activities [16,17]. The aim of the present study was to isolate the bioactive compound(s) from Af that responsible for the anti-inflammatory activity of the extract and to explain its anti-inflammatory activities in microglial cells.…”

Section: Downregulation Of Microglial Activation By Achillolide Amentioning

confidence: 99%

Downregulation of Microglial Activation by Achillolide A

et al. 2015

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Chronic inflammation has been implicated in the pathogenesis of various neurodegenerative diseases. During the neuroinflammatory process, microglial cells release neurotoxic and proinflammatory mediators. In the present study, using activity-guided fractionation, we have purified an anti-inflammatory compound determined by spectroscopic methods to be a sesquiterpene lactone named achillolide A from Achillea fragrantissima (Forsk.) Sch. Bip. In primary cultures of lipopolysaccharide-activated microglial cells, achillolide A inhibited the lipopolysaccharide-induced levels of proinflammatory and toxic mediators including glutamate, nitric oxide, matrix metalloproteinase-9, cyclooxygenase-2, induced nitric oxide synthase, interleukin-1β, and tumor necrosis factor-α. Achillolide A also exhibited an antioxidant capacity, as was shown in a cell free system as well as by its ability to reduce intracellular reactive oxygen species levels in microglial cells. Thus, achillolide A might have therapeutic potential for treatment of neurodegenerative diseases and deserves further studies.

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Section: Downregulation Of Microglial Activation By Achillolide Amentioning

confidence: 99%

Downregulation of Microglial Activation by Achillolide A

et al. 2015

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“…In order to characterize the ability of pulichalconoid B to protect against H 2 O 2 -induced oxidative stress, we assessed changes in cell viability using a model in which oxidative stress was induced by the addition of H 2 O 2 to cultures of primary astrocytes. Exposure of normal primary astrocytes to H 2 O 2 resulted in a time- and concentration-dependent death of astrocytes at 20 h after exposure [53]. To determine the optimal concentration of pulichalconoid B needed for a protective effect, astrocytes were preincubated with different concentrations of this molecule.…”

Section: Resultsmentioning

confidence: 99%

Protective and Antioxidant Effects of a Chalconoid fromPulicaria incisaon Brain Astrocytes

Elmann

Telerman

Erlank

et al. 2013

Oxidative Medicine and Cellular Longevity

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Oxidative stress is involved in the pathogenesis of neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. Astrocytes, the most abundant glial cells in the brain, protect neurons from reactive oxygen species (ROS) and provide them with trophic support, such as glial-derived neurotrophic factor (GDNF). Thus, any damage to astrocytes will affect neuronal survival. In the present study, by activity-guided fractionation, we have purified from the desert plant Pulicaria incisa two protective compounds and determined their structures by spectroscopic methods. The compounds were found to be new chalcones—pulichalconoid B and pulichalconoid C. This is the first study to characterize the antioxidant and protective effects of these compounds in any biological system. Using primary cultures of astrocytes, we have found that pulichalconoid B attenuated the accumulation of ROS following treatment of these cells with hydrogen peroxide by 89% and prevented 89% of the H2O2-induced death of astrocytes. Pulichalconoid B exhibited an antioxidant effect both in vitro and in the cellular antioxidant assay in astrocytes and microglial cells. Pulichalconoid B also caused a fourfold increase in GDNF transcription in these cells. Thus, this chalcone deserves further studies in order to evaluate if beneficial therapeutic effect exists.

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“…Aerial parts of this achillea were used in folk medicine in Saudi Arabia, followed by the historical hero Achilles during the A.fragrantissima Trojan war period [11]. Various studies have been performed, and it illustrates that medicinal properties such as anti-neuroinflammatory [17], anti-angiogenesis [18], anti-metastasis [18], neuroprotection [19], myorelaxant [20], anti-inflammatory [21], anti-diabetic [22,23], anti-trypanosomal [24], anti-microbial [25,26], anti-oxidant [27], anti-proliferation [28], pro-apoptotic [28], cytotoxicity [9,27], and anti-cancer [29][30][31][32][33] effects through the difference solvent extract, prepared oil or phytochemical constituents isolation methods. Despite that, a deep mechanism needs to prove the effect of A.fragrantissima on apoptosis function.…”

Section: Discussionmentioning

confidence: 99%

Achillea fragrantissima (Forssk.) Sch.Bip instigates the ROS/FADD/c-PARP expression that triggers apoptosis in breast cancer cell (MCF-7)

Alasmari

2024

PLoS ONE

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Achillea fragrantissima is a shrub plant that belongs to the Asteraceae family in Arabia and Egypt. It is used as folk medicine and is a good source of phenolic acids, flavonoids, and some active compounds. To investigate the anti-cancer effect of A.fragrantissima on breast cancer MCF-7 cells and find the critical mechanism involved in apoptosis. The toxicity and pharmacokinetic studies of ethanolic extract of A.fragrantissima was examined for anti-breast cancer properties. In turn, cytotoxicity and cell viability were achieved by the MTT method. Furthermore, the trypan blue exclusion and microscopy examination proved the presence of apoptotic cells. Again, fluorescent staining such as AO/EtBr, DCFH-DA, Rho-123, and Hoechst-33342 reveals the cellular cytoplasmic disciplines upon A. fragrantissima effect. Moreover, cellular functioning tests like wound healing, colony formation, and Transwell invasion assay were demonstrated. In addition, the qRT-PCR technique authenticates the A. fragrantissima -induced apoptotic network genes (Caspase-3, Caspase-8, Caspase-9, Cytochrome c, BCL-2, BID, BAX, PARP, PTEN, PI3K, and Akt) expression were evaluated. Mainly, the Immunoblot technique proved the expressed level of apoptotic proteins such as cleaved PARP, CYCS, and FADD. This study confirmed that the A. fragrantissima exerts cytotoxicity at 20 μg/mL for 24 hrs in MCF-7 cells. Also, decreases cellular viability, producing apoptotic cells and damaged cellular surfaces with dead matter. Consequently, it creates ROS species accumulation, loss of mitochondrial membrane potential, and fragmentation of DNA in MCF-7 cells. Furthermore, it arrests cell migration, induces colony-forming ability loss, and suppresses cell invasion. In addition, A. fragrantissima significantly upregulates genes such as caspase-3, 9, cytochrome c, BID, BAX, and PTEN while downregulating the Pi3K/ Akt signaling. Nonetheless, A.fragrantissima induced cleaved PARP, CYCS, and FADD proteins in MCF-7 cells to avail apoptosis.

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Extract of Achillea fragrantissima Downregulates ROS Production and Protects Astrocytes from Oxidative-Stress-Induced Cell Death

Cited by 7 publications

References 71 publications

Downregulation of Microglial Activation by Achillolide A

Downregulation of Microglial Activation by Achillolide A

Protective and Antioxidant Effects of a Chalconoid fromPulicaria incisaon Brain Astrocytes

Achillea fragrantissima (Forssk.) Sch.Bip instigates the ROS/FADD/c-PARP expression that triggers apoptosis in breast cancer cell (MCF-7)

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