Extract of curry powder and its components protect against diesel exhaust particle-induced inflammatory responses in human airway epithelial cells

Abstract

Measures for protecting against PM2.5 (particulate matter with aerodynamic diameters ≤2.5 µm), which exacerbates respiratory diseases, have not been established. The present study investigated the effects of extracts of curry powder and its components on pro-inflammatory responses, extracellular and intracellular reactive oxygen species (ROS) production, induced by the PM2.5 component, DEP (diesel exhaust particles). Airway epithelial cells were exposed to DEP in the presence of curry powder, or its major and/or anti-inflammatory components, clove and turmeric. Curry powder, clove, and turmeric inhibited DEPinduced IL-6 release and extracellular ROS; in the absence of clove and turmeric, these effects of curry powder were mild but similar. Among the other curry spices, cinnamon decreased IL-6 and extracellular ROS, and coriander decreased IL-6 alone. This is the first report on the protective effects of extracts of curry powder and its components, against PM2.5-induced airway inflammation, which may be partly through inhibition of extracellular ROS.

Electrical stimulation waveform‐dependent osteogenesis on PVDF/BaTiO3 composite using a customized and programmable cell stimulator

“…To measure extracellular ROS, 1 mM CM‐H2DCFDA was hydrolyzed with 0.01 M NaOH in the dark for 30 min at 37°C. The treatment with NaOH converts H2DCFDA to H2DCF, which can react with extracellular ROS to form fluorescent DCF (Honda et al, 2019). At the stipulated time, the culture media were isolated without cells and incubated with the hydrolyzed fluorescent probe for 1 h in the dark before measuring the fluorescence intensity using Excitation/Emission at 485 nm/535 nm.…”