Extract of Prunella Vulgaris Spikes Inhibits HIV Replication at Reverse Transcription in vitro and can Be Absorbed from Intestine in vivo

Kageyama, Seiji; Kurokawa, Mineo; Shiraki, Kimíyasu

doi:10.1177/095632020001100207

Cited by 30 publications

(25 citation statements)

References 24 publications

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“…Prunella vulgaris has been used as a traditional medicine for treatment of herpetic keratitis (22). Recent studies have reported that Prunella vulgaris extracts have anti-oxidant and anti-microbial properties (7,8). We previously reported that administration of PVAE to rats significantly reduced postprandial hyperglycemia in ICR mice (11).…”

Section: Discussionmentioning

confidence: 97%

“…P. vulgaris contains different bioactive components, including complex carbohydrates, hydrophobic metabolites (triterpenes), polysaccharides and Rosmarinic acid (5,6). Numerous studies have reported that extracts of P. vulgaris have properties which are involved in anti-oxidative stress, anti-microbial invasion (7,8), anti-inflammatory responses (9) and anti-DNA damage and caspase-3 activity (10). We previously reported that PVAE significantly reduces postprandial hyperglycemia in ICR mice (11).…”

Section: Introductionmentioning

confidence: 99%

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Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Gao

et al. 2012

Experimental and Therapeutic Medicine

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Abstract. We previously reported that Prunella vulgaris aqueous extract (PVAE) promotes hepatic glycogen synthesis and decreases postprandial hyperglycemia in ICR mice. Inflammatory cytokines play a critical role in the pathogenesis of diabetes. This study was designed to examine whether PVAE has a protective effect on IL-1β-induced apoptosis in INS-1 cells. INS-1 pancreatic β cells were plated at 2x10 6 /ml and treated with PVAE (100 µg/ml) 30 min before the cells were challenged with IL-1β (10 ng/ml). Untreated INS-1 cells served as control. INS-1 cell cytotoxicity was examined by MTT and lactate dehydrogenase (LDH) activity assays. Caspase-3 activity and activation of the apoptotic signaling pathway were analyzed by western blotting. NF-κB binding activity was examined by EMSA. The levels of inflammatory cytokines in the supernatant were measured by ELISA. IL-1β treatment significantly induced INS-1 cell death by 49.2%, increased LDH activity by 1.5-fold and caspase-3 activity by 7.6-fold, respectively, compared with control cells. However, PVAE administration significantly prevented IL-1β-increased INS-1 cell death and LDH activity and attenuated IL-1β-increased caspase-3 activity. Western blot data showed that PVAE also significantly attenuated IL-1β-increased Fas, FasL and phospho-JNK levels in the INS-1 cells. In addition, PVAE treatment significantly attenuated IL-1β-increased NF-κB binding activity and prevented IL-1β-increased TNF-α and IL-6 expression in INS-1 cells. Our data suggest that PVAE has a protective effect on IL-1β-induced INS-1 cell apoptosis. PVAE also attenuates IL-1β-increased NF-κB binding activity and inflammatory cytokine expression in INS-1 cells. PVAE may have a benefit for type I diabetic patients.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Gao

et al. 2012

Experimental and Therapeutic Medicine

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“…Prunella vulgaris L, a commonly used Chinese medicinal herb, also known as self-heal, has a wide range of reported medicinal activities [27,28] . This herb with antioxidant, antimicrobial and anti-inflammatory properties has a long history of use as a remedy for cardiovascular diseases [29,30] .…”

Section: Discussionmentioning

confidence: 99%

Steroid-like compounds in Chinese medicines promote blood circulation via inhibition of Na+/K+-ATPase

Chen¹,

Chung²,

et al. 2010

Acta Pharmacol Sin

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Aim: To examine if steroid-like compounds found in many Chinese medicinal products conventionally used for the promotion of blood circulation may act as active components via the same molecular mechanism triggered by cardiac glycosides, such as ouabain. Methods: The inhibitory potency of ouabain and the identified steroid-like compounds on Na + /K + -ATPase activity was examined and compared. Molecular modeling was exhibited for the docking of these compounds to Na + /K + -ATPase. Results: All the examined steroid-like compounds displayed more or less inhibition on Na + /K + -ATPase, with bufalin (structurally almost equivalent to ouabain) exhibiting significantly higher inhibitory potency than the others. In the pentacyclic triterpenoids examined, ursolic acid and oleanolic acid were moderate inhibitors of Na + /K + -ATPase, and their inhibitory potency was comparable to that of ginsenoside Rh2. The relatively high inhibitory potency of ursolic acid or oleanolic acid was due to the formation of a hydrogen bond between its carboxyl group and the Ile322 residue in the deep cavity close to two K + binding sites of Na + /K + -ATPase. Moreover, the drastic difference observed in the inhibitory potency of ouabain, bufalin, ginsenoside Rh2, and pentacyclic triterpenoids is ascribed mainly to the number of hydrogen bonds and partially to the strength of hydrophobic interaction between the compounds and residues around the deep cavity of Na + /K + -ATPase. Conclusion: Steroid-like compounds seem to contribute to therapeutic effects of many cardioactive Chinese medicinal products. Chinese herbs, such as Prunella vulgaris L, rich in ursolic acid, oleanolic acid and their glycoside derivatives may be adequate sources for cardiac therapy via effective inhibition on Na + /K + -ATPase.

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“…The aqueous and methanol extractions of P. vulgaris used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR) [57]. It has been reported that extracts of the spike of P. vulgaris exhibit anti-HIV activity at the adsorption and reverse transcription stages [58]. The anti-HIV-1 activity of aromatic herbs in Labiatae was evaluated in vitro, P. vulgaris aqueous extracts inhibited giant cell formation in co-culture of Molt-4 cells with and without HIV-1 infection and showed inhibitory activity against HIV-1 reverse transcriptase (RT) [59] [60].…”

Section: Antibacterial and Antivirus Activitiesmentioning

confidence: 99%

Research Progress on the Chemistry and Pharmacology of Prunella vulgaris Species

Ge¹,

Wang²,

Zhang³

et al. 2014

OALib

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An exhaustive literature survey on the secondary metabolites of widely distributed Prunella vulgaris L. species has been carried out. Triterpenoids, sterols, phenylpropanoids, flavonoids, coumarins, fatty acids, volatile oils and carbohydrates along with some other compounds, have been reported from this species. Many of these compounds have been found to possess significant biological properties, including antihypertensive activity, anticancer activity, anti-lipidemic activity, antibacterial and antivirus activities, anti-inflammatory activities, hypoglycemic effects, heptaprotective effects, antineoplastic activities, etc. P. vulgaris might offer a rich natural source with relative cheap price for developing new anti-neoplastic drugs.

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Extract of Prunella Vulgaris Spikes Inhibits HIV Replication at Reverse Transcription in vitro and can Be Absorbed from Intestine in vivo

Cited by 30 publications

References 24 publications

Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Prunella vulgaris aqueous extract attenuates IL-1β-induced apoptosis and NF-κB activation in INS-1 cells

Steroid-like compounds in Chinese medicines promote blood circulation via inhibition of Na+/K+-ATPase

Research Progress on the Chemistry and Pharmacology of Prunella vulgaris Species

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