Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin

Abstract

Lilium candidum L. extract (LE) is well known in folk medicine for the treatment of burns, ulcers, inflammations and for healing wounds. This work aims to clarify whether the genotoxic potential of the radiomimetic antibiotic zeocin (Zeo) could be modulated by LE. Our results indicate that LE exerts no cytotoxic, DNA-damaging and clastogenic activity in in Chlamydomonas reinhardtii, Pisum sativum L. and Hordeum vulgare L. test systems over a broad concentration range. Weak but statistically significant clastogenic effects due to the induction of micronuclei and chromosome aberrations have been observed in H. vulgare L. after treatment with 200 and 300 μg/mL LE. To discriminate protective from adverse action of LE different experimental designs have been used. Our results demonstrate that the treatment with mixtures of LE and Zeo causes an increase in the level of DNA damage, micronuclei and “metaphases with chromatid aberrations” (MwA). Clear evidence has been also obtained indicating that pretreatment with LE given 4 h before the treatment with Zeo accelerates the rejoining kinetics of Zeo-induced DNA damage in P. sativum L. and C. reinhardtii, and can decrease clastogenic effect of Zeo measured as frequencies of micronuclei and MwA in H. vulgare L. Here, we show for the first time that LE can modulate the genotoxic effects of zeocin. The molecular mode of action strongly depends on the experimental design and varies from synergistic to protective effect (adaptive response–AR). Our results also revealed that LE-induced AR to zeocin involves up-regulation of DSB rejoining in C. reinhardtii and P. sativum L. cells.

Liliumcompounds kaempferol and jatropham can modulate cytotoxic and genotoxic effects of radiomimetic zeocin in plants and human lymphocytesIn vitro

“…In our study, the protective effect was shown to depend on the experimental design and the test systems. The data correlate with the results for whole L. candidum extract (Kopaskova et al, ), suggesting that jatropham and kaempferol most probably play a key role in the protective effect. The antimutagenic effect of the whole Lilium extract in barley appears to depend to a greater extent on the experimental design, which could be due to other compounds.…”

“…On the basis of our preliminary experiments with the whole Lilium extract (Kopaskova et al, ), the most appropriate concentration ranges, treatment times and experimental designs were applied for both test systems. The concentration of jatropham and kaempferol used in H. vulgare was 100 μg mL −1 and in human lymphocytes, 10 μg mL −1 .…”

“…The radiomimetic zeocin (Zeo) (Cas No. : 11006-33-0, Invi-troGen), which belongs to the bleomycin family of antibiotics, was used as a standard mutagenic factor, well investigated and described in our previous studies (Chankova et al, 2007;Dimova et al, 2009;Kopaskova et al, 2012).…”

“…Our previous study showed that whole extract of L. candidum containing flavonoid and alkaloid components has a potential to modulate the genotoxic effect of the radiomimetic zeocin (Kopaskova et al, ).…”

Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo

“…Among the highly efficient ones are natural herbal extracts that comprise polyphenolic compounds. Natural plant extracts are now gaining more pharmacological attention, as many previously unexplored plant species appear to demonstrate a wide range of activities, such as anti-cancer activities (Vitalone et al 2003a(Vitalone et al , 2003b(Vitalone et al , 2006Scheck et al 2006), DNA protective and antimutagenic activities (Miadokova et al 2010;Kopaskova et al 2011), anti-inflammatory activities (Talhouk et al 2007), and anti-aging activities (Kim et al 2004). Among them are extracts from the aerial parts of Epilobium angustifolium (EA) (Oenotheraceae).…”