2023
DOI: 10.3390/bios13100913
|View full text |Cite
|
Sign up to set email alerts
|

Extract2Chip—Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance

Ana C. F. Paiva,
Ana R. Lemos,
Philipp Busse
et al.

Abstract: Modern drug discovery relies on combinatorial screening campaigns to find drug molecules targeting specific disease-associated proteins. The success of such campaigns often relies on functional and structural information of the selected therapeutic target, only achievable once its purification is mastered. With the aim of bypassing the protein purification process to gain insights on the druggability, ligand binding, and/or characterization of protein–protein interactions, herein, we describe the Extract2Chip … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2023
2023
2023
2023

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 52 publications
(68 reference statements)
0
1
0
Order By: Relevance
“…Recently, Paiva et al described a similar capturing approach with recombinant proteins from cellular extracts carrying an AviTag, which can directly bind to a Streptavidin SPR-chip after cellular biotinylation. Subsequently, the binding kinetics of the captured protein binding its target can be directly measured [21]. In 2012, an assay setup already demonstrated the capturing of antibodies directly from cell-culture supernatants utilizing appropriate immobilized species-specific capture antibodies.…”
Section: In Silico-determined Assay Settings Successfully Proven In V...mentioning
confidence: 99%
“…Recently, Paiva et al described a similar capturing approach with recombinant proteins from cellular extracts carrying an AviTag, which can directly bind to a Streptavidin SPR-chip after cellular biotinylation. Subsequently, the binding kinetics of the captured protein binding its target can be directly measured [21]. In 2012, an assay setup already demonstrated the capturing of antibodies directly from cell-culture supernatants utilizing appropriate immobilized species-specific capture antibodies.…”
Section: In Silico-determined Assay Settings Successfully Proven In V...mentioning
confidence: 99%