Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

Divyashree, M S; Shamala, T. R.

doi:10.1007/s12088-010-0013-1

Cited by 20 publications

(6 citation statements)

References 19 publications

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“…About 92% of P(3HB) could be quickly extracted from the fragile cells with 1 N aqueous NH 3 (pH 11.4) at 45°C for 10 min. The same phenomenon was observed with B. flexus [56]. The cells grown in the inorganic salts medium suffered from the absence of diaminopimelic acid (DAP) and decreased concentrations of other amino acids.…”

Section: Supercritical Fluid (Scf)supporting

confidence: 67%

“…The cell wall strength of these microorganisms could be compromised by modifying the composition of the growth medium. Cell fragility mechanism is not only restricted to Gram negative microorganisms but could be also exploited for Gram positive microorganisms [56]. Page and Cornish [55] demonstrated that the addition of fish peptone to the cultivation medium of A. vinelandii UWD led to the formation of large, pleomorphic, osmotically sensitive cells while high molecular weight P(3HB) synthesis was enhanced.…”

Section: Supercritical Fluid (Scf)mentioning

confidence: 99%

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Isolation and recovery of microbial polyhydroxyalkanoates

Kunasundari¹,

Sudesh²

2011

Express Polym. Lett.

249

162

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Abstract. The deleterious environmental impacts caused by plastic wastes have attracted worldwide concern. The biobased and biodegradable polyhydroxyalkanoate (PHA) appears to be one of the potential candidates to replace some conventional plastics. However, high production cost of PHAs has limited their market penetration. The major cost absorbing factors are the upstream fermentation processes and the downstream PHA recovery technologies. The latter significantly affects the overall process economics. Various recovery technologies have been proposed and studied in small scales in the laboratory as well as in industrial scales. These include solvent extraction, chemical digestion, enzymatic treatment and mechanical disruption, supercritical fluid disruption, flotation techniques, use of gamma irradiation and aqueous two-phase system. This paper reviews all the recovery methods known to date and compares their efficiency and the quality of the resulting PHA. Some of the large-scale production of PHA and the strategies employed to reduce the production cost are also discussed.

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Section: Supercritical Fluid (Scf)supporting

confidence: 67%

Section: Supercritical Fluid (Scf)mentioning

confidence: 99%

Isolation and recovery of microbial polyhydroxyalkanoates

Kunasundari¹,

Sudesh²

2011

Express Polym. Lett.

249

162

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“…Sodium hypochloride has a strong non-selective oxidizing property that can digest the NPCM (Yu and Chen, 2006). Sequential treatment with sodium hypochloride and a mixture of surfactants promoted the recovery of PHA with 50% reduced cost when compared to solvent extraction (Yu, 2009;Divyashree and Shamala, 2010). Even though chemical digestion has a low operating cost, it is considered unattractive due to complications in wastewater treatment and also the cost involved in the utilization of surfactants (Kunasundari and Sudesh, 2011).…”

Section: Chemical Treatmentmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

120

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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“…Priestia flexa (previously Bacillus flexus) could be isolated from soil (Divyashree and Shamala 2010) and marine sediment (Al Farraj et al 2020) samples. In this study, the P. flexa strain DNP2401 was found to be an amylase-producing bacterium.…”

Section: Discussionmentioning

confidence: 99%

Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand

KLINFOONG¹,

THUMMAKASORN²,

UNGWIWATKUL³

et al. 2022

Biodiversitas

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Abstract. Klinfoong R, Thummakasorn C, Ungwiwatkul S, Boontanom P, Chantarasiri A. 2022. Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand. Biodiversitas 23: 5519-5531. Mangrove forests are a potential ecosystem for the isolation of various economic enzymes derived from mangrove-associated bacteria. The knowledge of amylase-producing bacteria isolated from mangrove forests in the Southeast Asian region has been scarce. This study aimed to investigate the isolation, genetic identification, and activity characterization of amylase-producing bacteria from mangrove soils in Thailand. The amylase-producing bacteria isolated from mangrove soils in the present were genetically belong to the genera Bacillus, Desulfurella, Peribacillus, Priestia, and Pseudomonas. Several amylase-producing bacteria such as Bacillus proteolyticus, Desulfurella, Pseudomonas entomophila, and Pseudomonas putida found in this study have hardly ever been reported. The Bacillus paralicheniformis strain DNP0507 was the most active amylolytic bacterium with 2.395 ± 0.133 U/mg of amylase activity. The optimum temperature and pH for amylolytic activity were determined to be 50°C at a pH of 7.0 with a thermal stability range of 20-60°C at a neutral pH of 7.0-8.0. The enzyme activity was significantly enhanced by Cu2+, Co2+, and Pb2+ and was inhibited considerably by a chelating agent EDTA. Finally, the most active amylolytic B. paralicheniformis strain DNP0507 could be applied in baking industries, food industries, and starchy waste valorization.

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Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

Cited by 20 publications

References 19 publications

Isolation and recovery of microbial polyhydroxyalkanoates

Isolation and recovery of microbial polyhydroxyalkanoates

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Diversity and activity of amylase-producing bacteria isolated from mangrove soil in Thailand

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