Extracting and Physicochemical Properties of Carotenoprotein from Shrimp Processing Waste by Proteases-Mediated Hydrolysis

Abstract

In this study, carotenoprotein from Paci c white shrimp (Litopenaeus vannamei) processing waste was extracted with the aid of alcalase (CP-A) and pepsin (CP-P) at various levels (0-4 units/100g waste) for different times (0-240 min). Recovery of carotenoids and protein increased with increasing enzymes levels (3 unit/g) and hydrolysis times until 180 min (p<0.05). The extracted carotenoprotein by pepsin and alcalase consisted of 72.11-75.32% protein contents and carotenoids were in the range of 330-530 µg/g samples. The phenylalanine, lysine, methionine and valine as essential amino acids were high at CP-A and CP-P. The dominant non-essential amino acids in carotenoproteins were aspartic acid, glutamic acid, glycine and alanine. It was rich in mono and polyunsaturated fatty acids. The CP-A showed higher DHA and EPA (8.52 and 6.49%) than CP-P (5.55 and 5.49%). The saturated fatty acids were reduced after enzymatic hydrolysis and contents were higher in carotenoproteins. The extracted samples showed a signi cant amount of mineral contents. Sodium, phosphorus, magnesium and potassium contents were found to be higher in CP-A. The lead and copper reduced as a result of hydrolysis. Therefore, carotenoprotein from processing residue of paci c white shrimp could be used as the value-added nutritious enriching food or feed powder. Statement Of NoveltyExtraction of carotenoprotein could be valorize shrimp waste and reduce environmental problems. These residues are one of the problems of shrimp processors in developing countries. Lots of researchers evaluated the extraction of carotenoprotein under various conditions in their work. In the present work, carotenoproteins were prepared by two types of common enzymes.The obtained data can be useful in the option of the nd a suitable method to producing carotenoprotein for animal feed or food. It suggests that other ways is better to investigate and a comparison could be helpful.