Extraction and Analysis of Gigantol from Dendrobium officinale with Response Surface Methodology

Zheng, Siyan; Zhu, Yingpeng; Chen, Jiao; Shi, M.; Wei, Lianping; Zhou, Yang; Jin, Qing; Cai, Yongping

doi:10.3390/molecules23040818

Cited by 19 publications

(7 citation statements)

References 28 publications

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“…The p-values of the lack-of-fit were 0.9071 and 0.0562, respectively, and each was not significant (p > 0.05) relative to the pure error. This shows that the quadratic model is statistically suitable for predicting responses [50]. The coefficients of variation (CV) were determined as 4.13% and 9.75% for each model.…”

Section: Optimization Of Thermal-alkaline Pretreatment By Rsmmentioning

confidence: 83%

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

2021

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Orange peel, which is a by-product of oranges, contains carbohydrates that can be converted into sugars and used in the fermentation process. In this study, the thermal alkaline pretreatment process was chosen because of its simplicity and lesser reaction time. In addition, the reaction factors were optimized using response surface methodology. The determined optimal conditions were as follows: 60.1 g/L orange peels loading, 3% KOH and 30 min. Under the optimal conditions, glucan content (GC) and enzymatic digestibility (ED) were found to be 32.8% and 87.8%, respectively. Enzymatic hydrolysis was performed with pretreated and non-pretreated orange peels using three types of enzyme complex (cellulase, cellobiase and xylanase). The minimum concentrations of enzyme complex required to obtain maximum ED were 30 FPU (filter paper unit), 15 CBU (cellobiase unit), and 30 XNU (xylanase unit) based on 1 g-biomass. Additionally, ED of the treated group was approximately 3.7-fold higher than that of the control group. In conclusion, the use of orange peel as a feedstock for biorefinery can be a strategic solution to reduce wastage of resources and produce sustainable bioproducts.

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Section: Optimization Of Thermal-alkaline Pretreatment By Rsmmentioning

confidence: 83%

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

2021

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“…ANOVA was used to evaluate the optimization results. A small p-value indicates a significant influence on the response variables [ 7 ]. The model had a correlation p value of 0.0003 < 0.01, which indicated that the optimization model was significant.…”

Section: Resultsmentioning

confidence: 99%

“…A confirmatory experiment was performed based on the optimal extraction conditions obtained from the aforementioned experiments. A method was considered credible if the percentage error was less than 10% [ 7 ]. The average experimental value for the flavonoid extraction efficiency model was 35.45 ± 0.34 mg/50 mL.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

Lü

Yang

Zhan

et al. 2019

International Journal of Analytical Chemistry

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In order to establish the extraction technology of flavonoids from Dendrobium officinale leaves, a method combining Plackett–Burman design (PBD), steepest ascent design, and central composite design was developed to optimize the extraction of flavonoids. In addition, the tyrosinase activity inhibition of flavonoids was further tested in vitro. PBD results showed that ethanol concentration and number of extractions were key factors. Response surface methodology (RSM) indicated that the optimal extraction conditions were 78% ethanol concentration, six extraction times, 2 h, and 1:50 solid-liquid ratio. Under these conditions, the total flavonoid content could reach 35 mg/50 mL. In vitro tyrosinase experiment, the extracted total flavonoids had better inhibitory effect on tyrosinase activity than β-arbutin, and its inhibition rate for monophenolase and diphenolase exceeded 100% and 70%, respectively. These results indicate that RSM can effectively improve the extraction of flavonoids from Dendrobium officinale leaves and the flavonoids have the prospect of being applied to foods and cosmetics.

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“…Gigantol is a bibenzyl compound extracted from the Thai orchid, Dendrobium draconis ( 5 ). In China, gigantol is an active ingredient commonly extracted from Dendrobium officinale , which is a valuable Chinese herbal medicine ( 6 , 7 ). Plants of the Dendrobium genus have been reported to display immunomodulatory, neuroprotective, antioxidant and antitumor effects ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non‑small cell lung cancer

Cai

Hao

et al. 2021

Exp Ther Med

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Non-small cell lung cancer (NSCLC) is a common type of cancer, with a mortality of >80% worldwide. Gigantol is a bibenzyl compound that displays anticancer activity. The aim of the present study was to determine the biological activity of gigantol in NSCLC and to elucidate the underlying molecular mechanism of its action. The expression of DEK proto-oncogene (DEK) was measured in NSCLC tissues and cell lines by reverse transcription-quantitative PCR (RT-qPCR). The results suggested that DEK levels were significantly increased in NSCLC tissues and cell lines compared with adjacent non-tumor tissues and BEAS-2B normal bronchial epithelial cells, respectively. A549 cells were exposed to a series of gigantol concentrations (0, 25, 50 and 100 µM) and transfected with DEK small interfering RNA. The results of cell viability measured by MTT assay indicated that gigantol significantly decreased cell viability. Additionally, cell proliferation was assessed by CCK-8 and apoptosis was measured by flow cytometry. In comparison with the control group, gigantol treatment inhibited cell proliferation and promoted apoptosis, whereas DEK knockdown increased gigantol-induced suppression of proliferation and acceleration of apoptosis. Additionally, DEK overexpression reversed gigantol-induced effects on proliferation and apoptosis. Moreover, compared with the control group, gigantol treatment decreased Ki-67 and Bcl-2 expression levels, increased Bax expression levels and inactivated the Wnt/β-catenin signaling pathway, as assessed by RT-qPCR and/or western blot. DEK knockdown further increased gigantol-induced effects, but DEK overexpression reversed gigantol-induced effects. To conclude, the results of the present study suggested that gigantol inhibited cell proliferation and induced apoptosis by decreasing Ki-67 and Bcl-2 expression, increasing Bax expression and activating the Wnt/β-catenin signaling pathway by regulating DEK. The present study indicated the therapeutic potential of gigantol in patients with NSCLC. In addition, DEK may serve as a novel therapeutic target to enhance the effects of gigantol treatment.

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Extraction and Analysis of Gigantol from Dendrobium officinale with Response Surface Methodology

Cited by 19 publications

References 28 publications

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

Improved Sugar Recovery from Orange Peel by Statistical Optimization of Thermo-Alkaline Pretreatment

Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity

Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non‑small cell lung cancer

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