Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra)

Syahputra, Gita; Hariyatun, Hariyatun; Firdaus, Muhammad; Santoso, Pugoh

doi:10.18343/jipi.26.3.319

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…The amount of type-1 collagen content in sea cucumbers and Tilapia-skin can vary depending on the type. 15 In contrast to previous studies, compared with other collagen sources such as catfish-skin, snakehead fish-skin, Tilapia-skin and from other mammalian skins, the percentage of collagen yield from sea cucumbers is slightly high. 16 The source of collagen was found to be higher in some types of sea cucumbers.…”

Section: Resultscontrasting

confidence: 69%

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Safira¹,

Rani²,

Puspitasari³

et al. 2022

Pharmacogn. J.

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The dried sea cucumbers were washed with distilled water, dissected, and soaked in a solution of 0.5 N NaCl, 0.2 N -mercaptoethanol, 50 mM EDTA, and 0.1 N Tris-HCl. Next, the mixture was centrifuged at 8000 rpm and added with 0, 1 N NaOH. The sediment was centrifuged again and washed with distilled water. The supernatant obtained, added pepsin (1 g/100 g collagen) with 0.5 N acetic acid, centrifuged, and added NaCl to 0.6 M. By centrifugation, the sediment was redissolved in 0.5

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Section: Resultscontrasting

confidence: 69%

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Safira¹,

Rani²,

Puspitasari³

et al. 2022

Pharmacogn. J.

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“…Furthermore, the ratio of the amide III wavenumber to 1,454 cm -1 were 1.13 (with EDTA) and 1.17 (without EDTA), suggesting that the collagen triple helix structure is intact [39]. EDTA can chelate mineral ions without cleaving and breaking the triple helix structure of Yellowstripe skin collagen [40]. Therefore the FTIR spectra of products have no significant difference with each other [41].…”

Section: Resultsmentioning

confidence: 93%

The Effect of Ethylenediaminetetraacetic Acid (EDTA) and Pepsin Enzyme Addition on The Characteristics of Yellowstripe Fish (Selaroides Leptolepis) Collagen and Gelatin

Pradana,

Warsito,

Dahliyanti

2023

Proceedings of the International Conference on Sustainable Engineering, Infrastructure and Development, ICO-SEID 2022, 23-24 No

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The importance of collagen and gelatin has been increasing recently due to their wide applicability in various fields. Marine organisms provide promising alternatives as both collagen and gelatin sources compared to their traditional bovine and porcine counterparts due to better properties, high abundance, and health and religious reasons. In this research, collagen and gelatin were acid-extracted from the skin of Yellowstripe fish (Selaroides leptolepis) with the addition of Ethylenediaminetetraacetic Acid (EDTA) and pepsin enzyme. Fourier-transform infrared spectroscopy (FTIR) showed that all as-extracted products contain all characteristic functional groups of collagen or gelatin. The addition of 5 wt.% of Pepsin increased the yield of collagen and gelatin to the optimum values of 7.19 wt.% and 3.57 wt.%, respectively. Ethylene Diamine Tetra-acetic Acid (EDTA) pretreatment successfully lowers the mineral content down to 0.27 wt.% for collagen and 1.39 wt.% for gelatin. The Yellowstripe fish collagen and gelatin were stable until the denaturation temperature of 59.90℃ and 74.66℃.

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“…Prior to extraction, three sea cucumbers H. fuscocinerea and S. variegatus were sampled from the waters of the Thousand Islands National Park, cleaned using clean water, then the guts of the sea cucumbers were removed and cut into cubes. After being cut according to shape and size, samples of sea cucumbers were dried in an oven at 60 °C for 30 minutes (Syahputra et al, 2021). 20 g of dry sea cucumber samples were extracted by maceration method using 96% ethanol with a volume of 1:5 (w/v) for 3 times 24 hours.…”

Section: Work Procedures Extractionmentioning

confidence: 99%

Antioxidant Activity of Gamat (Stichopus variegatus) and Milk Sea Cucumbers (Holothuria fuscocinerea) from the Thousand Islands National Park Waters

Siregar,

Bachtiar,

Nurhayati

et al. 2023

JAFH

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The frequent use of synthetic antioxidant compounds can cause degenerative diseases, especially consumers, so that natural antioxidant compounds are needed to reduce the risk of disease. One of the biotas that may contain potential as natural antioxidants is sea cucumber. Sea cucumbers are marine invertebrates that have therapeutic properties whose bioactive content has the potential as antioxidants. Thus, this study aims to determine the potential of two species of sea cucumber (Stichopus variegatus and Holothuria fuscocinerea) as natural antioxidant candidates as an alternative to synthetic antioxidants by knowing the bioactive content and analyzing the antioxidant activity of the sample. The research was started from April 1 to September 29, 2021 with methods including sampling in the waters of the Thousand Islands National Park, extraction, antioxidant testing, testing for total compound content, and data analysis on sample extracts of S. variegatus and H. fuscocinerea. The results of the DDPH antioxidant test and β-carotene bleaching assay after the one-way ANOVA test showed that the antioxidant activity value was P < 0.05, which means that there was a significant difference in sample concentration on antioxidant activity. In the method and -carotene bleaching assay, the sample extracts of S. variegatus and H. fuscocinerea were 46.37% and 45.75%, respectively. Based on the value of the antioxidant test results and bioactive content, it can be concluded that the sample extracts of S. variegatus and H. fuscocinerea have very weak antioxidant activity so that they cannot be used as alternative natural antioxidants to replace synthetic antioxidants.

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Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra)

Cited by 5 publications

References 34 publications

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

The Effect of Ethylenediaminetetraacetic Acid (EDTA) and Pepsin Enzyme Addition on The Characteristics of Yellowstripe Fish (Selaroides Leptolepis) Collagen and Gelatin

Antioxidant Activity of Gamat (Stichopus variegatus) and Milk Sea Cucumbers (Holothuria fuscocinerea) from the Thousand Islands National Park Waters

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