2017
DOI: 10.4028/www.scientific.net/ssp.267.172
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Extraction and Characterization of Collagen Obtained from Collagen-Containing Wastes of the Leather Industry

Abstract: The study presents the extraction of collagen, a product of high value, from fleshings form hides. After testing several collagen extraction procedures we have proposed the simple and effective method to extract collagen from collagen-containing wastes of the leather industry. The unified method is based on the extraction of collagen using acetic acid in the presence of EDTA and included two repeated extraction stages. Qualitative analysis of the collagen using the disk-electrophoresis method showed a differen… Show more

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Cited by 12 publications
(4 citation statements)
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“…This process yielded acetic acid-soluble collagen at a rate of 6.15%. A SDS-PAGE analysis confirmed the collagen purity and identified it as type I, making this optimal for animal consumption [117].…”
Section: Recycling Of Wet Blue Waste and Its Potential Alternatives F...mentioning
confidence: 84%
“…This process yielded acetic acid-soluble collagen at a rate of 6.15%. A SDS-PAGE analysis confirmed the collagen purity and identified it as type I, making this optimal for animal consumption [117].…”
Section: Recycling Of Wet Blue Waste and Its Potential Alternatives F...mentioning
confidence: 84%
“…SDS-PAGE pattern of collagen extracted from waste of leather industry on 6% separating gel. Lane Kcontrol collagen (Savchuk et al, 2017), M -high molecular weight (MW, kDa) protein markers; 1-limed pelt, 2 -delimed pelt, 3 -fleshings K M 1 2 3…”
Section: Discussionmentioning
confidence: 99%
“…SDS-PAGE pattern of collagen extracted from waste of leather industry on 6% separating gel. Lane Kcontrol collagen (Savchuk et al, 2017), M -high molecular weight (MW, kDa) protein markers; 1-limed pelt, 2 -delimed pelt, 3 -fleshings…”
Section: Discussionmentioning
confidence: 99%