Extraction And Characterization Of Cycloartenol Isolated From Stems And Leaves of Coix Lacryma-Jobi L. And Its Potential Cytotoxic Activity

Abstract

[Background] Coix lacryma-jobi L. is a nourishing food and a traditional Chinese medicine and has been used for the treatment of neuralgia, inflammatory diseases, and rheumatism. Little is known about the anti-tumor of Coix lacryma-jobi L.. In this study, the cytotoxic effects of Coix lacryma-jobi L. on HeLa, HepG2, and SGC-7901 were evaluated.[Methods] The cytotoxic active compounds were isolated and extraction from the stems and leaves of Coix lacryma-jobi L. The structural identification of the compound was determined using ultraviolet spectroscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The cytotoxic activity in vitro effect of the compound was determined using CCK-8, Flow cytometry, and DNA Topo I inhibition experiments.[Results] A compound F2 was isolated and purified from the petroleum ether extract of Coix lacryma-jobi L. stems and leaves. It was identified as the cycloartenol. The minimum IC50 values of HeLa, HepG2 and SGC-7901 cells for this compound were 500, 537.7, and 336.8 μg/mL, respectively. The compound had pro-apoptotic effects on three types of tumor cells, and had a significant inhibitory effect on DNA topoisomerase I.[Conclusion] This study demonstrates that cycloartenol has good cytotoxic activity in vitro, suggesting that cycloartenol could be a potential candidate as a natural antitumor drug.

The critical role of the phytosterols in modulating tumor microenvironment via multiple signaling: A comprehensive molecular approach

“…Furthermore, the cycloartenol also inhibited oxidative stress and total ROS production via NF-κB signaling (Yu et al, 2021). Through inhibition of IκB kinase activation, this steroid blocked IκBα phosphorylation and degradation, thus suppressing p65 phosphorylation and nuclear translocation (Yu et al, 2021).…”

“…It inhibits the translocation of the p65 into the nucleus to induce the transcription of the pro-inflammatory mediators via increasing the IκB protein (Gupta & Ved, 2017). Cycloartenol is a triterpenoid sterol in other PSs that has been implicated in the inhibition of tumorigenesis by targeting the NF-κB and increasing the IkB inhibitor (Yu, Ye, Li, Li, & Huang, 2021). Furthermore, the cycloartenol also inhibited oxidative stress and total ROS production via NF-κB signaling (Yu et al, 2021).…”

“…Cycloartenol is a triterpenoid sterol in other PSs that has been implicated in the inhibition of tumorigenesis by targeting the NF-κB and increasing the IkB inhibitor (Yu, Ye, Li, Li, & Huang, 2021). Furthermore, the cycloartenol also inhibited oxidative stress and total ROS production via NF-κB signaling (Yu et al, 2021). Through inhibition of IκB kinase activation, this steroid blocked IκBα phosphorylation and degradation, thus suppressing p65 phosphorylation and nuclear translocation (Yu et al, 2021).…”

“…Guggulsterone inhibits skin tumorigenesis in the SENCAR mice by targeting the MAPKs and NF‐κB signaling, which subsequently, influenced the genes concerned with cell growth and proliferation (Abdellatef et al, 2021; Kim et al, 2015). The PSs cycloartenol inhibit the glioma cell growth and proliferation by inducing cell arrest and MAPKs‐mediated apoptosis, especially p38 unit (Adewole & Ishola, 2019; Yu et al, 2021). Withanolides also exhibited anti‐cancer activities by interacting with the MAPKs signaling protein, and its upstream and downstream proteins to alter the expression of genes concerned with the cell cycle regulation and apoptosis (Akhtar, Baig, Haq, Rajeeve, & Cutillas, 2020; Subramanian et al, 2018).…”

Green Synthesis of Copper Oxide Nanoparticles Using Coix lacryma jobi Leaves Extract and Screening of its Potential Anticancer Activities

“…The non-toxic form of nanoparticles have been used in cancer therapy [39,40]. Considering the biological activities of Coix lacryma jobi, which acts as antioxidant [41], cytotoxic activities [42] and its leaves extracts at a different medium that inhibits various cancer cells such as Hela, HepG2, and SGC-7901 [43,44], hepatoprotective and anti-inflammatory activity [45] antitumor activities in human cervical cells [46] antibacterial and anthelmintic activities [47] were observed. However, the preparation of green CuO NPs using Coix lacryma jobi leaves has not yet been reported to the best of our knowledge.…”