Extraction and Characterization of Flavanol-Rich Nutraceuticals Based on High-Performance Liquid Chromatography

Abstract

Flavanols are a class of polyphenols that have become the focus of much research because of their medicinal properties. For this paper, a new method to determine polyphenols in nutraceutical samples was developed, based on solid-liquid extraction and further analysis by liquid chromatography. Analytes were recovered by ultrasound-assisted extraction, using a mixture of methanol/water/hydrochloric acid (70:29:1, v:v:v) as the solvent. Reversed-phase high-performance liquid chromatography with ultraviolet/visible (HPLC-UV/Vis) and fluorescence detection (HPLC-FLD) were developed to determine the content of the principal flavanols. The HPLC-FLD method showed better selectivity and sensitivity, so it was selected for further studies. The analytical parameters established with FLD were satisfactory in terms of linearity (R2 ≥ 0.993), repeatability (RSD% ≥ 2.2), and limits of detection of 0.1 mg L−1 or below. The nutraceutical samples were analyzed by HPLC-FLD to determine the compositional profiles of flavanols, then they were subsequently treated using chemometric methods, such as principal component analysis (PCA). Results showed that the study of compositional profiles from the selected flavanols was sufficient for description and discrimination purposes. The cranberry samples were the richest in flavanols, procyanidin A2 being the principal flavanol, and artichoke samples the least rich.

Systematic Review of the Literature to Inform the Development of a South African Dietary Polyphenol Composition Database

“…Some limitations include the exclusion of date restrictions when conducting the search and thus some data that met the inclusion criteria were analysed using methods that are currently not considered good quality. The Folin-Ciocalteu method has been critiqued as it lacks specificity for phenolic compounds and is known to overestimate values when other reducing agents are also present in the foods, compared to the more specific high-performance liquid chromatography (HPLC) methods [55]. Nonetheless, as this was the first stage in the process leading to the development of a national polyphenol database and in the absence of any prior knowledge of the extent of data available, it was deemed important to obtain as much data as possible.…”

Chitosan Surface-Modified PLGA Nanoparticles Loaded with Cranberry Powder Extract as a Potential Oral Delivery Platform for Targeting Colon Cancer Cells

“…Samples were taken at different intervals over 24 h, diffused through a syringe Millipore filter (0.4 µm), and evaluated using HPLC at a λ max of 280 nm, based on Casanella et al’s method [ 34 ]. The amount of CBPE permeated was calculated by measuring the surface area of the intestinal sac, which was modeled as a cylinder [ 35 ].…”

Research Progress on Extraction and Detection Technologies of Flavonoid Compounds in Foods

“…The results showed that the method had high precision (%RSD 0.2 to 1.9%) and accuracy (100.7 to 102.9%) and was successfully used to evaluate flavanols and proanthocyanidins in different cocoa products. Vidal-Casanella et al [131] developed a reversed-phase high-performance liquid chromatography method with HPLC-UV/Vis and FLD to determine the content of major flavanols. The results showed that the HPLC-FLD method has better selectivity and sensitivity, and the detection limit is 0.1 mg L −1 .…”