Extraction and characterization of pepsin-soluble collagen from different mantis shrimp species

Abstract

The objective of this study was to investigate the yield and characteristics of collagen protein extracted from the muscle of four different species of mantis shrimp: Miyakella nepa, Harpiosquilla harpax, Erugosquilla woodmasoni, and Odontodactylus cultrifer. Mantis shrimp muscle was extracted by using a pepsin-solubilization technique, with 0.5 M acetic acid and 5% pepsin enzyme. The highest collagen yield was from M. nepa muscle (0.478 ± 0.06%), which was significantly greater (p < 0.05) than that from H. harpax, O. cultrifer, and E. woodmasoni (0.313 ± 0.03%, 0.123 ± 0.02%, and 0.015 ± 0.00%, respectively). The freeze-dried collagen appeared as thin fibers, and formed an opaque film. The pepsin-soluble collagen (PSC) from four mantis shrimp species was analyzed by gel electrophoresis. The results showed that all species of mantis shrimp contained type I collagen, consisting of β, α1, and α2 subunits with average molecular weights of 250, 145, and 118 kDa, respectively. The study of the solubility of collagen showed that, for NaCl, collagen had the highest relative solubility in 2% NaCl (80.20 ± 4.95%). In contrast, the solubility decreased at higher NaCl concentrations. However, in terms of pH, collagen had the highest relative solubility at pH 3 (91.32 ± 5.14%), and its solubility decreased at higher pH. FT-IR spectroscopy was used to compare the collagen with a model compound. Five wavenumbers in the spectrum for model collagen were identified: Amide A (3,406-3,421 cm -1 ), amide B (2,916-2,940 cm -1 ), amide I (1,639-1,640 cm -1 ), amide II (1,539-1,570 cm -1 ), and amide III (1,234-1,250 cm -1 ).

Effects of proteases on textural softening and changes in physical property of harpiosquillid mantis shrimp (Harpiosquilla raphidea) during the iced storage

“…TCA‐SPC has been used to indicate protein degradation (Chen et al ., 2022). In the muscle, MHC, collagen and actin were hydrolysed as witnessed by the increases in TCA‐SPC throughout the storage of 3 days (Sriket et al ., 2012a; Singh & Benjakul, 2018; Hiransuchalert et al ., 2021). Therefore, proteins in HMS meat were prone to hydrolysis by several proteases, particularly when storage time increased.…”

Extraction and Physico–Chemical Characterization of Chitosan from Mantis Shrimp (Oratosquilla nepa) Shell and the Development of Bio-Composite Film with Agarose

“…They are abundant along the coast in the southern part of Thailand and have become economically important due to their palatability and useability in a variety of cooking. By the year 2021, the total of all species of mantis shrimp caught was 700–800 tons per year, with an estimated value of USD 1.18 million [ 1 ]. Processing of mantis shrimp generates large amount of leftover material, mainly exoskeleton.…”

Impact of Prior Pulsed Electric Field and Chitooligosaccharide Treatment on Trypsin Activity and Quality Changes in Whole and Beheaded Harpiosquillid Mantis Shrimp during Storage in Iced Water

“…The amide A region of the NH stretching exclusively localized on the protein could be detected at a peak between 3290–3400 cm −1 [ 23 ]. Similarly, amide B, representing the C-H stretch for the mantis shrimp, could be found at 2916–2940 cm −1 [ 13 ]. In general, no differences in the amide I, II and III regions were found among any of the samples.…”

“…The sample (5th abdomen segment) was cut in transversal section and was stored in Davidson’s fixative solution and subsequently subjected to histological analysis using the method detailed by Hiransuchalert et al [ 13 ] and fixed in Paraplast TM (Histosec ® , Merck, Darmstadt, Hesse, Germany). The staining and histological analysis were accomplished.…”