“…Firstly, the extraction yields of DMY were studied through a series of single factor tests with the important extraction parameters, including water concentration (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, and 90% v/v), shaken time (0.5, 1, 1.5, 2, 2.5, 3 and 3.5 h), liquid-solid ratios (5,10,20,30,40 and 50 mL g −1 ) and temperature (30, 35, 40, 45, 50, 55 and 60 C). According to the above single factor experimental results, a Box-Behnken design (BBD) based on RSM was applied for the optimization of the extraction conditions with the Design-Expert 8.0.6 Trail soware (Stat-Ease, Minneapolis, MN, USA).…”