Extraction and In Vitro Skincare Effect Assessment of Polysaccharides Extract from the Roots of Abelmoschus manihot (L.)

Abelmoschus manihot is an important medicinal and edible plant known for its functional secondary metabolites. However, little is known about the key genes involved in production of secondary metabolites in A. manihot. This is largely due to a lack of effective gene expression detection systems for A. manihot, and thus screening of real-time PCR reference genes is a prerequisite. In this study, 11 candidate reference genes were screened and cloned from A. manihot, and their expression stability was evaluated in different tissues using four algorithms: geNorm, NormFinder, BestKeeper, and RefFinder. The expression stability of eIF and PP2A1 was the highest, while that of tubulin alpha (TUA) was the lowest. The combined use of the two most stable reference genes, eIF and PP2A1, met the experimental requirements for normalizing gene expression in A. manihot. Furthermore, the gene expression of transcription factors bHLH147 and bHLH148 was further validated by data normalization. This study identified potential reference genes in different A. manihot tissues, paving the way for functional gene analysis and dissecting metabolite regulation mechanisms in A. manihot.

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Harmonizing In Vitro Techniques for Anti-Aging Cosmetic Ingredient Assessment: A Comprehensive Review

Vinardell,

Maddaleno,

Mitjans

2024

Cosmetics

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Skin grows old due to intrinsic factors, such as age and associated hormonal changes, and external factors, like solar radiation and chemical substances to which we are exposed. With age, skin shows thinning, laxity, pallor, increased dryness, and the appearance of wrinkles, attributed to a decrease in collagen and elastin produced by fibroblasts. Several theories explain skin aging, including the free radical formation, neuroendocrine, and mitochondrial decline theories. Based on a scientific understanding of skin behavior, different in vitro methods are used to evaluate the effects of new ingredients in cosmetics. Commonly used methods include anti-collagenase, anti-elastase, and anti-hyaluronidase activity alongside techniques utilizing skin cells or 3D models. Although these methods are recognized and widely used, they lack standardization. In this review, a literature search has been conducted to examine the characteristics and variations of these methods across the laboratories. A key issue identified in this review is that many papers provide insufficient detail regarding their protocols. Moreover, the number of studies using cells is less significant than the ones determining enzyme inhibition. Our findings revealed that, in many cases, there is limited information available, underscoring the urgent need to initiate a comprehensive standardization process for the methodologies used to demonstrate anti-aging activity.

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Extraction and In Vitro Skincare Effect Assessment of Polysaccharides Extract from the Roots of Abelmoschus manihot (L.)

Cited by 3 publications

References 37 publications

Extraction of collagen from bovine tannery solid waste preserving original conformation via radical initiation and hydrogen bond reformation

Extraction of collagen from bovine tannery solid waste preserving original conformation via radical initiation and hydrogen bond reformation

Screening and validation of optimal real-time PCR reference genes for Abelmoschus manihot

Harmonizing In Vitro Techniques for Anti-Aging Cosmetic Ingredient Assessment: A Comprehensive Review

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