Due to the diminishing supply of fossil fuels, biodiesel is gaining popularity as a feasible fuel. It is produced through the fermentation of Napier liquid with the help of bakery yeast. The pH test, viscosity analysis, multi-cylinder, and other tests yielded favourable results when compared to traditional fuels such as diesel. In the Viscosity analysis, we compare the pure and blended fuel with regular diesel, it was discovered that pure ethanol has reduced dynamic viscosity, which improves when blended with diesel. For the multi-cylinder engine performance test, adding 10 % ethanol to gasoline results in a bit of improvement in efficiency over regular diesel. The efficiency is greater than the previous one when it reaches 20 %. As a significance, we blended ethanol up to 20 % with normal diesel to obtain optimum results. Because complete combustion is achieved due to oxygen contained in ethanoldiesel blends, the exhaust gas temperature of the blends is found to be lower, and Nitrogen emission is higher compared to diesel.