Extraction and physico-chemical characterization of a versatile biodegradable polysaccharide obtained from green algae

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

“…Carbohydrate polymers of marine green algae have recently been exploited for various applications [6,7,8,9,10,11,12], and green algal polysaccharides have emerged as rich and important sources of bioactive natural compounds with a wide range of physiological and biological activities [13,14], including immunomodulation, anti-inflammation, antioxidant, anticoagulant, and antitumor. Sulfated polysaccharides (SPs) of green seaweeds, are chemically and physicochemically different from those of land plants [6], and may have special physiological effects on the human body [15].…”

Ulvan: A Versatile Platform of Biomaterials from Renewable Resources

“…Ulvan represents the major biopolymeric fraction of the cell wall having the function of maintaining the osmolar stability and protection of the cell (Paradossi et al, 2002). As usually found in polysaccharides present into the cell walls, Ulvan is present in close association with proteins and the conventional methods of extraction and purification resulted not completely effective in the removal of the protein fraction even after a specific deproteinization protocol (Alves et al, 2010). Extraction is conventionally achieved by using warm water solution (80-90°C) containing ammonium oxalate as divalent cation chelator and the recovery of Ulvan is generally obtained by precipitation in ethanol.…”

Detection of a Minor Amorphous Phase in Crystalline Etoricoxib by Dynamic Mechanical Analysis: Comparison with Raman Spectroscopy and Modulated Differential Scanning Calorimetry

“…Since that publication, several authors have evaluated powders using a similar holder or modified holders for DMA. [30][31][32][33][34]36 Using Royall's method, the quantitation of amorphous material in physical blends of amorphous/crystalline etoricoxib was determined by DMA. There is a linear relationship between the baselinecorrected DMA tan(δ) peak height (DMA strength) and the amorphous content.…”

“…Recent publications on the development of stainless steel pockets has allowed for the detection of phase, glass transition temperatures (T g s), and mechanical properties such as storage and loss moduli, and tan(δ) properties of pharmaceutical powders. [29][30][31][32][33][34][35] The DMA technique using an optimized powder DMA holder has been shown to be more sensitive than DSC for the evaluation of T g s of common pharmaceutical excipient powders such as hydroxypropylmethylcellulose, as well as felodipine, a model drug. 30 Gearing et al 35 used DMA and the stainless steel pocket to determine T g s in frozen biomaterials such as heparin, albumin, influenza antigen, and human plasma, as well as in the generally used lyophilization excipients such as trehalose, lactose, and dextran.…”

“…36 Similarly, T g s of ulvan, a polysaccharide biomaterial, which could not be observed by DSC, were determined by DMA. 34 In another example, T g s of poly (D,L-lactic acid) and inclusion complexes with alphacyclodextrin, in powder form, were determined using the stainless steel pockets. 32 The novel DMA method was more sensitive than DSC to detect the amorphous regions and T g s in lyophilized powders of lysozyme.…”