Extraction and purification of a highly thermostable alkaline caseinolytic protease from wastes Penaeus vannamei suitable for food and detergent industries

Abstract

A novel thermostable protease was purified from Penaeus vannamei from Persian Gulf to homogeneity level using ammonium sulfate precipitation and anion-exchange chromatography. The purified protease showed a single band on native and SDS-PAGE with a molecular weight of 24kDa on SDS-PAGE. The enzyme showed the broad highest catalytic activity for hydrolysis of the substrate with maximal activity at pH 7 and 80°C. Activity of the enzyme was inhibited by Hg(2+), Zn(2+) Co(2+) and Cu(2+), while protease activity was increased in the presence of Fe(2+) and Mn(2+) by factors of 173% and 102%, respectively. Enzyme shows a broad substrate specificity and hydrolyzes both natural and synthetic substrates. Based on the Michaelis-Menten plots, the Km with casein as substrate was 16.8μM and Vmax was 82.6μM/min. The enzyme, derived from L. vannamei, possesses unique characteristics and could be used in various industrial and biotechnological applications.

Characterization of biosynthesized chitosan nanoparticles fromPenaeus vannameifor the immobilization ofP. vannameiprotease: An eco-friendly nanobiocatalyst

“…Protease enzyme isolated from P. vannamei has been purified according to a previous procedure. [1] All other chemicals were of reagent grade and purchased from Merck (Darmstadt, Germany).…”

“…Activation energy for the enzymatic activity was 6.50 kcal mol −1 K −1. [1] To the best of our knowledge, studies of P. vannamei protease immobilized on chitosan nanoparticles have not been reported. In the present work, chitosan nanoparticles were prepared by the ionization gelation methodology.…”

“…Enzyme is a large biological molecule that regulates chemical reaction in numerous biological processes, including signal transduction, gene expression, immune response, metastasis, metabolism, pharmaceutical and medical fields, food and environmental industry, biofuel area, life science studies as well as biosensors. [1][2][3][4][5][6][7] The interest in biocatalysts for chemical production continues to grow because they generally have high stereo, chemo, and region-selectivity. They also offer an efficient and environmentally friendly catalyst without the need of high pressures, temperatures, and harsh chemical environments.…”

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

“…However, for practical application, crude enzyme extracts have to be purified to improve their apparent activity and catalytic efficiency [15]. The reported enzyme purification processes such as multi-step solvent partitioning [16], the aqueous two-phase system [17], ammonium sulfate precipitation [18] and sequential chromatographic process [19,20] are tedious and time-consuming. In addition, these steps also incur extra downstream processing costs.…”

Produção de proteases por fungos endofíticos isolados de plantas do Cerrado

“…São muitas as possíveis aplicações das enzimas proteolíticas. É comum a utilização destas em diversas indústrias tais como de alimentos (8-10), detergente (11,12), couro (13), farmacêutica e de cosméticos (14) (15).…”