Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

Abstract

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD).Electronic supplementary materialThe online version of this article (doi:10.1007/s40502-014-0094-7) contains supplementary material, which is available to authorized users.

Phycocyanobilin reduces brain injury after endothelin‐1‐ induced focal cerebral ischaemia

“…Afterwards, cell debris was separated by centrifugation at 10 000 g for 20 minutes (Eppendorf Centrifuge 5810 R, Eppendorf, Hamburg, Germany). Soluble proteins obtained after centrifugation were precipitated with ammonium sulphate salt at 50% saturation . Proteins were sedimented by centrifugation at 10 000 g for 20 minutes and subsequently treated with pure methanol for 16 hours at 42°C.…”

Spray-dried Spirulina platensis as an effective ingredient to improve yogurt formulations: Testing different encapsulating solutions

“…Phycocyanin is an accessory pigment of chlorophyll and belongs to the phycobiliprotein family. It is soluble in water and can be easily extracted from S. platensis (Chaiklahan, Chirasuwan, & Bunnag, 2012), usually by the freezing/thawing method (forms ice crystals that cause cell disruption, facilitating the pigments' release), employing different solvents (Abalde, 1998;Kumar, Dhar, Pabbi, Kumar, & Walia, 2014;Soni, Kalavadia, Trivedi, & Madamwar, 2006). In this work, water was the solvent that allowed to extract the highest amount of phycocyanin (1.44 mg/mL, Table 1), which was significantly higher than the concentrations obtained with acetate buffer, phosphate buffer, and ethanol (Table A1, supplementary material), and also higher than the concentrations obtained in similar studies (Paliwal, Bhayani, Maurya, & Mishra, 2015).…”

Phycobilins and Phycobiliproteins Used in Food Industry and Medicine

“…However, extraction from cyanobacteria is not simple due to their special multilayered cell wall and the presence of contaminants, therefore, extraction usually results either in high purity or high efficiency. Isolation consists typically of two phases: (1) liberation of intracellular content by pretreatment to yield a crude extract (i.e., by precipitation, centrifugation, or other treatments including microwave-or ultrasound-assisted extraction [75,76]), (2) isolation/purification step(s) that separate the pigment-protein complexes by conventional methods [5,13,61,73,[77][78][79][80][81]. Phycocyanin is mostly extracted from Arthrospira [79,[81][82][83] (Fig.…”

“…After extraction a combination of gel adsorption and ion exchange chromatography can be used for purification. However, simpler (one-step), faster and more cost-efficient purification protocols are under development for this species (e.g., [13,81]), and extraction and purification from other species were also reported (e.g., [77,84], reviewed in [64]). Pure phycoerythrin can be obtained from the unicellular red microalga, Porphyridium cruentum [77], but protocols for other red micro-or macroalgal species have been also reported [85][86][87][88].…”

“…Phycobiliprotein colorants (especially phycocyanin) are considered non-toxic and non-carcinogenic and are used as blue colorants in some countries (e.g., in Japan, Australia [96], Thailand and China [81]) to color cosmetics (like lipstick and eye liners) [107] and food such as fermented milk products, ice creams and sorbets, popsicles, milk shakes and desserts, other dairy products, sweet cake decorations, chewing gum, candies, wasabi, different soft and alcoholic beverages [61]. Phycocyanin (isolated from Arthrospira) was first marketed under the brand name Linablue® by Dainippon Ink & Chemicals Inc. (Japan, current name: DIC Corporation) in 1977-1980 [108] (Fig.…”