Extraction and Purification of Calcitonin

Baghdiantz, A; Foster, G. V.; Edwards, Anthony; Kumar, M. Sunil; Slack, Ella; Soliman, Hanan A.; MacIntyre, I.

doi:10.1038/2031027a0

Cited by 65 publications

(21 citation statements)

References 4 publications

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“…Etant donnt que l'injection thyrocalcitonine chez diffkrents animaux [2,4] et chez de thyrocalcitonine provoque une hypocalckmie plus l'homme [15] conduit a reprendre l'ktude des relaimportante chez l'animal en croissance que chez l'anitions, partiellement connues, entre l'activitk de la ma1 adulte, il convenait d'autre part de comparer glande thyro'ide et le mktabolisme calcique.…”

Section: Introductionunclassified

Effet hypocalcémiant de la thyrocalcitonine chez l'enfant normal et l'enfant hypothyroïdien

et al. 1967

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Section: Introductionunclassified

Effet hypocalcémiant de la thyrocalcitonine chez l'enfant normal et l'enfant hypothyroïdien

et al. 1967

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“…Baghdiantz et al (1964), Hirsch et al (1964), and Tashjian and Warnock (1967) also demonstrated the peptide nature and the purconcomitant loss of biological activity of their relatively impure -CT preparations. These hypocalcemic preparations were readily in-.activated by incubating them with a variety of proteolytic enzymes such as trypsin and pepsin.…”

Section: Biologicai Assay and Radioimmunoassay Biological Ass~ymentioning

confidence: 94%

“…An effective alternative method for partial purification of porcine CT, utilizing dried, defatted thyroid tissue which subse---f!ue.ntly underwent salt fractionation ( 2-J M NaCl) and gel fil tration on Sephadex G-100 columns was reported by Baghdiantz et al (1964). Baghdiantz et al (1964), Hirsch et al (1964), and Tashjian and Warnock (1967) also demonstrated the peptide nature and the purconcomitant loss of biological activity of their relatively impure -CT preparations.…”

Section: Biologicai Assay and Radioimmunoassay Biological Ass~ymentioning

confidence: 99%

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Oeltgen¹,

L'Heureux²

1975

Horm Metab Res

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Mitochondria, nu dei, and microsomes obtained from ovine thyroid slices wh ich had been incubated in Krebs-Ringer bicarbonate buffer containing 4.8, 11.5 or 26.0 mg% concentration of calcium were assayed for calcitonin activity in rats. The microsomal preparatioa was the only particulate fraction to exhibit marked hypocalcemic activity and the hormonal activity of this fraction was enhanced by a 26.0 mg% calcium concentration in the incubation medium. The identity of the hypocalcemic species in the microsomal fraction with a calcitonin preparation 01' known potency was established by polyacrylamide gel eletrophoresis and by isoelectric focusing. The ovine microsomal calcitonin was resolved into two pro tein bands with isoelectric points (pl's) of 3.49 and 3.84. Both of these protein peaks exhibited hypocalcemic activity. The porcine standard calcitonin was resolved into two protein bands with pl's of 3.70 and 3.94.

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“…Thyrocalcitonin employed was made from acetone-dried pig thyroid tissue by the method of Baghdiantz, Foster, Edwards, Kumar, Slack, Soliman & MacIntyre (1964). Both crude and partially purified (Gudmundsson et al 1966) preparations were used, their potency being determined by 4-point bio-assay in rats (Kumar, Slack, Edwards, Soliman, Baghdiantz, Foster & Maclntyre, 1965) Solutions of 47CaC12 with a specific activity of 50-100 mc/g Ca were obtained from the Radiochemical Centre, Amersham, Bucks, and after appropriate dilution with saline solution or with saline thyro-calcitonin solution they were infused through the 'drug' tube when clearance determinations were to be made.…”

Section: Methodsmentioning

confidence: 99%

The effect of thyrocalcitonin on blood—bone calcium equilibrium in the perfused tibia of the cat

MacIntyre¹,

Parsons²,

Robinson³

1967

The Journal of Physiology

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SUMMARY1. In the isolated tibia of the cat, perfused with blood from its own carotid connected to popliteal artery via a pump, infusion of porcine thyrocalcitonin causes an arteriovenous difference in calcium concentration of up to 5 %, corresponding to net retention of calcium in bone.2. When taken in conjunction with determinations of radiocalcium clearance in the tibia, the effect is of the right order of magnitude to account for the hypocalcaemic action of thyrocalcitonin in the whole animal.3. Calcium retention was accompanied by a small increase in acidity of outflowing venous blood whereas verification of the hypothesis of calcium regulation by pH change in the bone substance would require a decrease.

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Extraction and Purification of Calcitonin

Cited by 65 publications

References 4 publications

Effet hypocalcémiant de la thyrocalcitonine chez l'enfant normal et l'enfant hypothyroïdien

Effet hypocalcémiant de la thyrocalcitonine chez l'enfant normal et l'enfant hypothyroïdien

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

The effect of thyrocalcitonin on blood—bone calcium equilibrium in the perfused tibia of the cat

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