Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes

Abstract

AIM To extract and purify the transforming growth factor β β β β β (TGF β β β β β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.

A focus on the role of platelets in liver regeneration: Do platelet-endothelial cell interactions initiate the regenerative process?

“…Transforming growth factor-β (TGF-β), the main terminator of hepatocyte proliferation [12], induces apoptosis in hepatocytes in vitro [14,15] and in vivo [16]. By contrast, no cell death occurs after PH, suggesting that the apoptotic pathways are suppressed during liver regeneration.…”

Effects of cytokines on carbon tetrachloride-induced hepatic fibrogenesis in rats

“…When activated, HSCs could proliferate and transform to a myofibroblast-like phenotype, expressing α-smooth muscle actin (α-SMA) and collagen types I, III and IV, fibronectin and proteoglycan [19] . It has been found several cytokines play roles by acting on HSC, and among them, TGF-β 1 is one of the most important cytokines involved in the fibrotic and cirrhotic transformation of the liver [20][21][22][23] . After a fibrogenic injury, expression of three forms of TGF-β is greatly upregulated in HSCs.…”

Growth factors from bovine milk and colostrum: composition, extraction and biological activities

“…Chromatography methods used for the separation of growth factors and IgG from milk, whey or colostrum. Milk basic protein (MBP) Whey Cationic-exchange (mono S) [216] TGF-β2 B o v i n e platelets Ethanol-acid extraction from platelets and CM sepharose [201] TGF-β, IGF-I Milk products Cation exchange resins + hydroxyapatite column [106] TGF-β1, TGF-β2 Bone tissue Cation-exchange resins + RP-HPLC, heparin column, thiophillic resin [98] TGF-β ? Heparin-like resin + butyl sepharose-like resin [57] 112 S.F.…”

“…Their fractionation approach based on cation-exchange chromatography has been used for the extraction of growth factors from bovine platelets [201,230], mice neuroblastomes [231], bone tissue [98] and porcine uterus [20].…”