Extraction and Quantification of Phytoestrogens in Foods Using Automated Solid-Phase Extraction and LC/MS/MS

Kuhnle, Gunter; Dell'Aquila, Caterina; Low, Yen-Ling; Kussmaul, Michaela; Bingham, Sheila

doi:10.1021/ac701732r

Cited by 67 publications

(75 citation statements)

References 58 publications

(91 reference statements)

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“…Isoflavones and other phytoestrogens of coffee samples are usually determined with the use of HPLC [47] or gas chromatography-mass spectrometry (GC-MS) [48,49] or using automatic SPE and LC-MS/MS [50]. To free the aglicones of isoflavones from glycosides or glycoside esters and quantify them, acid hydrolysis [47] or enzymatic procedure [51] was applied.…”

Section: Isoflavones: Daidzein Genistein and Formononetinmentioning

confidence: 99%

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Jeszka-Skowron

Zgoła‐Grześkowiak

Grześkowiak

2014

Eur Food Res Technol

125

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Section: Isoflavones: Daidzein Genistein and Formononetinmentioning

confidence: 99%

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Jeszka-Skowron

Zgoła‐Grześkowiak

Grześkowiak

2014

Eur Food Res Technol

125

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“…Foods were analysed as described previously (42) . In brief, foods were prepared, freezedried and extracted with 10 % aqueous methanol by volume.…”

Section: Analysis Of Foodsmentioning

confidence: 99%

Intakes and sources of isoflavones, lignans, enterolignans, coumestrol and soya-containing foods in the Norfolk arm of the European Prospective Investigation into Cancer and Nutrition (EPIC-Norfolk), from 7 d food diaries, using a newly updated database

Mulligan

Kuhnle

Lentjes

et al. 2012

Public Health Nutr.

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Objective: A diet rich in phyto-oestrogens has been suggested to protect against a variety of common diseases but UK intake data on phyto-oestrogens or their food sources are sparse. The present study estimates the average intakes of isoflavones, lignans, enterolignans and coumestrol from 7 d food diaries and provides data on total isoflavone, lignan and phyto-oestrogen consumption by food group. Design: Development of a food composition database for twelve phyto-oestrogens and analysis of soya food and phyto-oestrogen consumption in a populationbased study. Setting: Men and women, aged 40-79 years, from the general population participating in the Norfolk arm of the European Prospective Investigation into Cancer and Nutrition (EPIC-Norfolk) between 1993 and 1997, with nutrient and food data from 7 d food diaries. Subjects: A subset of 20 437 participants. Results: The median daily phyto-oestrogen intake for all men was 1199 mg (interquartile range 934-1537 mg; mean 1504 mg, SD 1502 mg) and 888 mg for all women (interquartile range 710-1135 mg; mean 1205 mg, SD 1701 mg). In soya consumers, median daily intakes were higher: 2861 mg in men (interquartile range 1304-7269 mg; mean 5051 mg, SD 5031 mg) and 3142 mg in women (interquartile range 1089-7327 mg; mean 5396 mg, SD 6092 mg). In both men and women, bread made the greatest contribution to phyto-oestrogen intake -40?8 % and 35?6 %, respectively. In soya consumers, vegetable dishes and soya/goat's/ sheep's milks were the main contributors -45?7 % and 21?3 % in men and 38?4 % and 33?7 % in women, respectively. Conclusions: The ability to estimate phyto-oestrogen intake in Western populations more accurately will aid investigations into their suggested effects on health.

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“…Other less-investigated compounds include prenylflavonoids, flavones, flavans and phytosterol esters [2]. These compounds are similar to 17β-estradiol in structure and function [3]. They have a dual-directional regulation function in the human body, meaning both anti-estrogen activity and estrogenic activity [4].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Daidzein, Genistein and Formononetin in Coffee by Capillary Zone Electrophoresis

et al. 2017

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Coffee is a favorite and beverage in Western countries that is consumed daily. In the present study, capillary zone electrophoresis (CE) was applied for the separation and quantification of three isoflavones including daidzein, genistein and formononetin in coffee. Extraction of isoflavones from the coffee sample was carried out by extraction and purification process using ether after the acid hydrolysis with the antioxidant butylated hydroxy-toluene (BHT). The experimental conditions of the CE separation method were: 20 mmol/L Na 2 HPO 4 buffer solution, 25 kV applied voltage, 3 s hydrodynamic injection at 30 mbar, and UV detection at 254 nm. The results show that the three compounds can be tested within 10 min with a linearity of 0.5-50 µg/mL for all three compounds. The limits of detection were 0.0642, 0.134, and 0.0825 µg/mL for daidzein, formononetin and genistein, respectively. The corresponding average recovery was 99.39% (Relative Standard Detection (RSD) = 1.76%), 98.71% (RSD = 2.11%) and 97.37% (RSD = 3.74%).

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Extraction and Quantification of Phytoestrogens in Foods Using Automated Solid-Phase Extraction and LC/MS/MS

Cited by 67 publications

References 58 publications

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Analytical methods applied for the characterization and the determination of bioactive compounds in coffee

Intakes and sources of isoflavones, lignans, enterolignans, coumestrol and soya-containing foods in the Norfolk arm of the European Prospective Investigation into Cancer and Nutrition (EPIC-Norfolk), from 7 d food diaries, using a newly updated database

Simultaneous Determination of Daidzein, Genistein and Formononetin in Coffee by Capillary Zone Electrophoresis

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