2000
DOI: 10.2144/00286bm06
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Extraction and Quantitative Determination of Callose from Arabidopsis Leaves

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Cited by 25 publications
(17 citation statements)
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“…Each reaction mixture contained 0.72 units of peroxidase and 77.6 mM luminol. Extraction and measurement of callose from Arabidopsis leaves were done as described (Kohler and Conrath, 2000). Arabidopsis leaves were harvested and pulverized after complete removal of chlorophyll with ethanol.…”
Section: Plant Materials Chemical Treatment and Pathogen Challengementioning
confidence: 99%
“…Each reaction mixture contained 0.72 units of peroxidase and 77.6 mM luminol. Extraction and measurement of callose from Arabidopsis leaves were done as described (Kohler and Conrath, 2000). Arabidopsis leaves were harvested and pulverized after complete removal of chlorophyll with ethanol.…”
Section: Plant Materials Chemical Treatment and Pathogen Challengementioning
confidence: 99%
“…This is well supported by the recent findings that the mpk4 mutant in Arabidopsis, which exhibits constitutive SAR, contains higher levels of detectable callose than the wild type plants (Østergaard et al 2002). In addition, Arabidopsis plants that established a resistant state as a result of the treatment with the SAR inducer, benzothiadiazole, enhanced callose deposition (Kohler et al 2002).…”
Section: Systemic Cellular Response and Oxidative Burst In Sarmentioning
confidence: 99%
“…Thus, AOK-30 treatment seemed to accelerate accumulation of callose, a heterogeneous b-1,3-glucan involved in a variety of plant developmental processes such as cell division, ripening of pollen mother cells 22) and deposition of wall appositions 25) . As reported by Kohler et al 22) , callose biosynthesis is induced in plants treated with various compounds of microbial origin, and callose deposition often serves as a reliable biochemical marker for activation of the plant defense response. Aist 25) distinguished the papillae that form upon microbial attack from wound plugs which serve as structural barriers against external physical or chemical stimuli.…”
Section: Resultsmentioning
confidence: 99%
“…Callose was extracted from AOK-30-treated and -untreated seedlings according to the method of Kohler et al 22) with slight modifications. Ten days after AOK-30 treatment about 0.5 g of seedling sample was immersed in 100% ethanol in 20-ml plastic tubes.…”
Section: Extraction and Analysis Of Callose In Aok-30-treated And -Unmentioning
confidence: 99%