Extraction and separation of a lysine-rich protein by formation of supramolecule between crown ether and protein in aqueous two-phase system

Oshima, Tatsuya; Suetsugu, Akinori; Baba, Yoshihiro

doi:10.1016/j.aca.2010.06.039

Cited by 40 publications

(24 citation statements)

References 58 publications

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“…With the use of ATPS, one can simultaneously carry out purification, extraction, and enrichment. ATPSs have become an important emerging technique for separation, concentration, and purification of proteins [19,20], cell organelles [21], and other biological products [22].…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid/Ammonium Sulfate Aqueous Two-phase System Coupled with HPLC Extraction of Sulfadimidine in Real Environmental Water Samples

Han

Wang

et al. 2011

Chromatographia

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Room temperature ionic liquids are novel solvents with a rather specific blend of physical and solution properties that make them of interest for applications in separation science. In this study, a green, simple, and sensitive sample pretreatment procedure coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of sulfadimidine (SM2) that exploits an aqueous two-phase system based on 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF 4 ) and ammonium sulfate ((NH 4 ) 2 SO 4 ) using a liquid-liquid extraction technique in real environmental water samples. The influences of the concentration of (NH 4 ) 2 SO 4 , pH value, temperature, and concentration of SM2 on the extraction efficiency of SM2 were determined. Under optimal conditions, the extraction efficiencies of SM2 were over 93.7%. Calibration curves yielded good linearity (R 2 = 0.9998) over the range 3-240 ng mL -1 , and the limit of detection and limit of quantification for analytes were 0.9 ng mL -1 and 3 ng mL -1 , respectively. The proposed method was successfully applied to the quantification of SM2 in water samples and recoveries were in the range of 101.2-107%.

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Section: Introductionmentioning

confidence: 99%

Ionic liquid/Ammonium Sulfate Aqueous Two-phase System Coupled with HPLC Extraction of Sulfadimidine in Real Environmental Water Samples

Han

Wang

et al. 2011

Chromatographia

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“…They are utilized to extract cations from aqueous solutions into hydrophobic, nonpolar organic solvents [5][6][7][8][9][10][11] and to carry these cations selectively through solvent-polymeric [12] liquid membranes [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Complexation study of dibenzo-18-crown-6 with UO2 2+ cation in binary mixed non-aqueous solutions

Razghandi

Rounaghi

Kakhki

2011

J Incl Phenom Macrocycl Chem

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The complexation reaction of macrocyclic ligand, dibenzo-18-crown-6 (DB18C6) with UO 2 2? cation was studied in ethylacetate-1,2-dichloroethane (EtOAc/ DCE), acetonitrile-1,2-dichloroethane (AN/DCE), methanol-1,2-dichloroethane (MeOH/DCE) and ethanol-1,2-dichloroethane (EtOH/DCE) binary solutions at different temperatures using the conductometric method. The conductance data show that the stoichiometry of the complex formed between DB18C6 and UO 2 2? cation is affected by the nature of the solvent systems. A non-linear behaviour was observed for changes of log K f of (DB18C6.UO 2 ) ?2 complex versus the composition of the binary mixed solvents. The values of thermodynamic quantities (DS°c, DH°c) for formation of (DB18C6.UO 2 ) ?2 complex were obtained from temperature dependence of the stability constant using the van't Hoff plots. The results show that in most cases, the complex is enthalpy stabilized and in all cases entropy stabilized and both parameters are affected by the nature and composition of the mixed solvents. In addition, the complex formation between dicyclohexyl-18-crown-6 (DCH18C6) and UO 2 2? cation was studied in pure AN and the results were compared with those of the (DB18C6.UO 2 ) ?2 complex.

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“…The phenol subunits are bridged via methyl groups and thus provide the characteristic vase-like shape of calixarenes (Ludwig 2000;Ludwig and Dzung 2002). Due to the presence of the hydrophobic cavity formed by the phenol units, calixarenes have been successfully employed for detection of a wide range of compounds including nucleosides (Guo et al 2004;Ludwig 2005;Cacciapaglia et al 2006), amino acids (Hassen et al 2007;Sugden et al 2008), or proteins (Oh et al 2005;Oshima et al 2010). Moreover, modification of the side groups allows one to prepare tailormade calixarenes with high affinity towards the specific target molecules (Baldini et al 2007;Sliwa and Deska 2011).…”

Section: Introductionmentioning

confidence: 99%

The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c

Vitovič

Subjaková²,

Hianik³

2013

gpb

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Abstract. We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix [6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at an air-water interface and complexes in mixed monolayers contained DPhPC. Calixarenes increase the elastic moduli of lipid monolayers as well as BLM. Cytochrome c (cyt c) specifically binds to CX by incorporation of the amino groups of lysine residues at the protein surface. This binding affected the physical properties of CX monolayers depending on their initial surface pressure. Addition of cyt c into the water subphase induced increase of surface pressure of CX monolayers at relatively low initial pressure (15 mN/m) when monolayer was in liquid expanded state (LE). This may be due to interaction of positively charged cyt c with negatively charged carboxylic groups of CX and also by its penetration into the air-water interface. However, much subtle changes were observed for higher initial surface pressure (20 and 35 mN/m) when monolayer is in liquid condensed (LC) and solid (S) state, respectively. Lysine induced substantially lower changes in surface pressure in comparison with that of cyt c.

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Extraction and separation of a lysine-rich protein by formation of supramolecule between crown ether and protein in aqueous two-phase system

Cited by 40 publications

References 58 publications

Ionic liquid/Ammonium Sulfate Aqueous Two-phase System Coupled with HPLC Extraction of Sulfadimidine in Real Environmental Water Samples

Ionic liquid/Ammonium Sulfate Aqueous Two-phase System Coupled with HPLC Extraction of Sulfadimidine in Real Environmental Water Samples

Complexation study of dibenzo-18-crown-6 with UO2 2+ cation in binary mixed non-aqueous solutions

The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c

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