2022
DOI: 10.1007/978-1-0716-2728-0_2
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Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

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Cited by 3 publications
(2 citation statements)
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“…They were centrifuged twice at 2000 g for 10’ at 4 °C and pellets were frozen in liquid nitrogen and stored at −80 °C until processed. Lipid extraction and parallel quantification of endocannabinoids was performed at the lipidomics unit of the Universitätsmedizin associated to the University of Mainz (Germany) as previously described [ 32 ].…”
Section: Methodsmentioning
confidence: 99%
“…They were centrifuged twice at 2000 g for 10’ at 4 °C and pellets were frozen in liquid nitrogen and stored at −80 °C until processed. Lipid extraction and parallel quantification of endocannabinoids was performed at the lipidomics unit of the Universitätsmedizin associated to the University of Mainz (Germany) as previously described [ 32 ].…”
Section: Methodsmentioning
confidence: 99%
“…However, they also serve as substrates for cyclooxygenases (Urquhart et al 2015 ), cytochrome P450 isoforms, and lipoxygenases (Ivanov et al 2021 ). These enzymes oxygenate the arachidonic acid backbone, which leads to the formation of an entirely novel array of bioactive lipids (Schwitter et al 2023 ). The bioactivities of these oxygenated endocannabinoids are different from those of the non-oxygenated parent compounds, and thus, enzymatic oxygenation modifies the functionality of the endocannabinoid system, which contributes to the pathogenesis of AD (Abate et al 2021 ).…”
Section: Apolipoprotein E and Alzheimer’s Diseasementioning
confidence: 99%