Extraction and Stability of Carotenoid-Containing Lipids from Hepatopancreas of Pacific White Shrimp (Litopenaeus vannamei )

Abstract

Impacts of extraction conditions on lipid and carotenoid yields from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) were investigated. Among single solvents (acetone, isopropanol and hexane) and their mixtures, a mixture of hexane and isopropanol (50:50, v/v) rendered lipids with the highest carotenoid yield (336.40 mg/kg hepatopancreas) with the extraction yield of 18.22% (w/w hepatopancreas) (P < 0.05). The use of hepatopancreas to solvent ratio of 1.0:4.5 (w/v) and three repetitions showed the highest carotenoid yield (363.94 mg/kg hepatopancreas) with the extraction yield of 18.08% (w/w hepatopancreas). Astaxanthin, astaxanthin diester and canthaxanthin were the major carotenoids found in lipids. When astaxanthin (2 mg/g lipid) was added into lipid, the oxidation was lowered, in comparison with the control during the storage at 30C for 10 days as indicated by lower ρ-anisidine value. Fourier transform infrared spectra study also revealed that triglyceride in lipids underwent oxidation to a lower degree when astaxanthin was incorporated. PRACTICAL APPLICATIONSShrimp hepatopancreas, a by-product from shrimp-processing industries, is one of the important sources of lipids and natural carotenoids, which are susceptible to oxidation. Their changes are associated with development of off-odor and loss in nutritive value. The recovery of lipid and carotenoid from hepatopancreas by an appropriate means would increase the revenue for shrimp-processing industries. Additionally, those value-added products could serve as the excellent source of nutrients as well as bioactive compounds.

Optimization of carotenoids extraction from blue crab (Portunus pelagicus ) and shrimp (Penaeus semisulcatus ) wastes using organic solvents and vegetable oils

“…For wet tissues, nonpolar solvents were inappropriate since their penetration through the hydrophobic mass surrounding the pigment is limited (Sachindra et al, ). Takeungwongtrakul Benjakul, Santoso, Trilaksani, and Nurilmala () extracted carotenoid from the hepatopancreas of Pacific white shrimp ( Litopenaeus vannamei ) by using acetone, isopropanol, and hexane and also by their mixtures. A 50:50 mixture of hexane and isopropanol had the highest carotenoid yield (336.40 µg g −1 hepatopancreas).…”

Chemical composition and physical properties of salted shrimp paste (Kapi) produced in Thailand

“…Carotenoid content of hepatopancreas of Pacific white LM S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 97 66 45 30 21 14.4 MW (kDa) Fig. 1 Protein patterns of various salted shrimp pastes, LM low-molecular weight marker, key: see Table 1 caption shrimp was 336.40 mg/kg sample (Takeungwongtrakul et al 2012a). Sachindra et al (2006) reported the highest carotenoid content (43.9 mg/kg sample) from head and carapace of Penaeus indicus when 50:50 (v/v) mixture of hexane and isopropanol was used as extracting medium.…”

Astaxanthin degradation and lipid oxidation of Pacific white shrimp oil: kinetics study and stability as affected by storage conditions

“…Oil was extracted from hepatopancreas following the method of Takeungwongtrakul et al (2014). The prepared hepatopancreas (20 g) was homogenized with 90 ml of cold solvent mixtures (isopropanol: hexane, 50: 50, v/v) (4°C) at the speed of 9500 rpm using an IKA Labortechnik homogenizer (Selangor, Malaysia) for 2 min at 4°C.…”